Anti-Folate Binding Protein/FBP antibody [LK26] (ab3361)

Reviews (1)Q&A (5)References (11)
Immunohistochemistry (Frozen sections) - Anti-Folate Binding Protein/FBP antibody [LK26] (ab3361)

    Key features and details

    • Mouse monoclonal [LK26] to Folate Binding Protein/FBP
    • Suitable for: IHC-Fr
    • Reacts with: Human
    • Isotype: IgG2a

    Overview

    • Product name

      Anti-Folate Binding Protein/FBP antibody [LK26]
      See all Folate Binding Protein/FBP primary antibodies

    • Description

      Mouse monoclonal [LK26] to Folate Binding Protein/FBP

    • Host species

      Mouse

    • Tested Applications & Species

      Application Species
      IHC-Fr
      Human
      See all applications and species data

    • Immunogen

      Tissue, cells or virus corresponding to Folate Binding Protein/FBP. Gestational choriocarcinoma cell line, Lu-75.

    • Positive control

      • Frozen Placenta or Ovarian Carcinoma (Negative control: Mouse serum or IgG1 isotype-matched negative control antibody, diluted to the same concentration as the primary antibody, should also be included in each run.)

    • General notes

      Previously labelled as Folate Binding Protein

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituents: 1% BSA, PBS
    • Concentration information loading...

    • Purity

      Affinity purified

    • Clonality

      Monoclonal

    • Clone number

      LK26

    • Isotype

      IgG2a

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3361 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-Fr
    Human
    Application Abreviews Notes
    IHC-Fr
    1/40.
    Notes
    IHC-Fr
    1/40.

    Target

    • Function

      Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.

    • Tissue specificity

      Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.

    • Involvement in disease

      Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.

    • Sequence similarities

      Belongs to the folate receptor family.

    • Post-translational
      modifications

      Eight disulfide bonds are present.
      The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.

    • Cellular localization

      Cell membrane. Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • adult antibody
        • Adult folate binding protein antibody
        • Adult folate-binding protein antibody
        • FBP antibody
        • Folate Binding Protein antibody
        • Folate Receptor 1 Adult antibody
        • Folate receptor 1 antibody
        • Folate Receptor 1 Precursor antibody
        • Folate receptor adult antibody
        • Folate receptor alpha antibody
        • Folate receptor antibody
        • FOLR antibody
        • FOLR1 antibody
        • FOLR1_HUMAN antibody
        • FR alpha antibody
        • FR-alpha antibody
        • FRalpha antibody
        • KB cells FBP antibody
        • MOV18 antibody
        • Ovarian cancer associated antigen antibody
        • Ovarian tumor associated antigen antibody
        • Ovarian tumor associated antigen MOv18 antibody
        • Ovarian tumor-associated antigen MOv18 antibody
        see all

      Images

      • Immunohistochemistry (Frozen sections) - Anti-Folate Binding Protein/FBP antibody [LK26] (ab3361)
        Immunohistochemistry (Frozen sections) - Anti-Folate Binding Protein/FBP antibody [LK26] (ab3361)
        ab3361 staining frozen placenta in IHC-F.

      References (11)

      Publishing research using ab3361? Please let us know so that we can cite the reference in this datasheet.

      ab3361 has been referenced in 11 publications.

      • Chen J & Li L Aberrant Expression of Folate Metabolism Enzymes and Its Diagnosis and Survival Prediction in Ovarian Carcinoma. Anal Cell Pathol (Amst) 2019:1438628 (2019). PubMed: 31049278
      • Shivange G  et al. A Single-Agent Dual-Specificity Targeting of FOLR1 and DR5 as an Effective Strategy for Ovarian Cancer. Cancer Cell 34:331-345.e11 (2018). PubMed: 30107179
      • Tesarova B  et al. Folic acid-mediated re-shuttling of ferritin receptor specificity towards a selective delivery of highly cytotoxic nickel(II) coordination compounds. Int J Biol Macromol 126:1099-1111 (2018). PubMed: 30605747
      • Siwowska K  et al. Folate Receptor-Positive Gynecological Cancer Cells: In Vitro and In Vivo Characterization. Pharmaceuticals (Basel) 10:N/A (2017). PubMed: 28809784
      • Oseledchyk A  et al. Folate-Targeted Surface-Enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detection of Microscopic Ovarian Cancer. ACS Nano 11:1488-1497 (2017). PubMed: 27992724
      View all Publications for this product

      Customer reviews and Q&As

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      1-6 of 6 Abreviews or Q&A

      Question

      I have a question about your products the Anti-Folate Binding Protein antibody (ab2107) and the Anti-Folate Binding Protein antibody [LK26] (ab3361), I was wondering how many runs (approximately) would you get from each product? Please email me at your earliest convience.

      Read More
      Answer

      It will depend on how you want to use the antibody and how much is required for each test . I updated the ab2107 datasheet to show suggested dilution ranges for the applications that are listed on the datasheet.

      WB: 1/500 - 1/5000.
      IP: 1/100.
      ELISA: 1/5000 - 1/20000.

      Untested applications (that is, not listed on the datasheet) will require optimization tests but I can suggest a range of dilutions to try for those too.

      The ab3361 datasheet has suggestions for dilutions for IHC and western blotting but the other applications that are listed were added based on publications that did not specify the dilutions or concentrations that were used.

      Read More

      Question

      Buenos dias,
      Nos parecebuena idea probar conotro anticuerpo. Vamos aprobar con la segunda opcion que nos recomiendas (ab125030).
      La dirección de envio es la misma que la del otro anticuerpo.
      Muchisimas gracias por vuestra ayuda,
      un saludo,

      Read More
      Answer

      Thank you for confirming these details and for your cooperation.

      As requested, I have issued a free of charge replacement with ab125030, the order number is *******.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Question

      Buenas tardes,
      Hemos cambiado las condiciones del western blott tal y como nos aconsejaste y seguimos sin tener resultados. Sin embargo hemos realizado una inmunocitoquímica en células de neuroblastoma y el anticuerpo ha marcado bien. No sabemoscuál puede ser el fallo. ¿que solución nos proponeis?
      Muchas gracias,

      Read More
      Answer

      Gracias por tu respuesta.

      Si te parece puedo enviaros para que probéis un anticuerpo diferente contra la misma proteína.

      Echarle un vistazo a los otros productos que tenemos disponibles contra Folate Binding Protein validados en WB en células humanas:

      https://www.abcam.com/Folate-Binding-Protein-antibody-ab67422.html

      https://www.abcam.com/Folate-Binding-Protein-antibody-EPR4708-2-ab125030.html

      La última de las opciones corresponde a un rabbit monoclonal, que suelen dar muy buenos resultados, y muestras más especificidad y sensibilidad que los mouse monoclonales.

      Si os interesa, os envío este anticuerpo de forma gratuita puesto que el ab3361 se encuentra aun en garantía.

      Quedo a la espera de tu respuesta para ayudaros con este problema.

      Read More

      Question

      Buenas tarde ,
      Adjunto el impreso. Espero que sirva de ayuda para poder solucionar el problema. No adjunto fotos porque las imagenes que salian al revelar el WB eran completamente negras con algo de ruido del secundario.
      un saludo,

      Read More
      Answer

      Muchas gracias por mandarnos el cuestionario relleno.

      Para intentar optimizar los resultados del anticuerpo hay una serie de sugerencias que me gustaría hacer. De cualquier manera, al estar al anticuerpo bajo garantía en caso de que las sugerencias no consiguieran optimizar el protocolo, estaré encantada de ofreceros un reemplazo del mismo, o un vale sin caducidad si preferís.

      - Para extraer la proteína podéis probar NP-40 como buffer de lisis. Este buffer está indicado para estudiar proteínas membranales. Podéis echar un vistazo al protocolo online donde se detalla más la información de este y otros buffers:

      https://www.abcam.com/index.html?pageconfig=resource&rid=11379#A1

      - Usar un control positivo siempre ayuda a entender el problema ocurrido. Si tuvierais alguna de las siguientes líneas celulares disponibles: JAR, HeLa, MCF7, 293T y A549 podrían servir como control.

      - Usar un método de bloqueo menos agresivo, como por ejemplo 5% BSA durante 1 o 2 horas a TA (en ese caso los anticuerpos diluirlos en TBST únicamente, o 1% BSA en TBST).

      -Simplemente a modo de comprobación, chequear que tanto el secundario como el sustrato enzimático están en perfectas condiciones y dan el resultado esperado en otros sistemas.

      Os propongo que probéis estas variaciones en el protocolo y en caso de seguir sin obtener el resultado esperado me volváis a contactar. Si el anticuerpo resultara haber salido defectuoso, al estar en garantía, os mandaríamos un vial nuevo gratuito o un reembolso en compensación.

      Así pues, os deseo mucha suerte, y espero vuestra respuesta.

      Read More

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Folate Binding Protein / FBP antibody [LK26]

       Poor
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (THP-1)
      Permeabilization
      No
      Specification
      THP-1
      Blocking step
      Protein Block (serum free) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 13 2008

      Question

      I have purchased your ab3361 two months ago and it works well (on western blot which you do not indicate). However I wanted to know the subtype of folate receptor recognized by this antibody (alpha, beta or both).

      Read More
      Answer

      Thank you for your enquiry. Ab3361 was generated using gestational choriocarcinoma cell line, Lu-75, as the immunogen. However, we have not conducted testing to determine whether the antibody is specific for one or the other subtype of the receptor. I'm pleased to hear that the antibody worked well for you in WB as it has not previously been tested in that application. We would be very grateful for you if you could submit an Abreview via the product datasheet. We always encourage customers to feed their results back to us, whether positive or negative, and make all information about each of our products available to researchers. If you send us an Abreview, we can offer you 50 Abcam Points (a further 100 Abpoints will be offered for an image). To find out more about our Loyalty Scheme and Abpoints, please visit our website and look at Your Account panel (left bottom section on our home page) or the Abreviews section on the product datasheet. You can submit the Abreview if you click on the Abreview section (top menu bar) on the on-line datasheet of this antibody. It is a very straightforward process. Should you need any help how to submit an Abreview, then please do not hesitate to contact us again. Please contact us again if you have any additional questions.

      Read More

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