Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab58920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Lane 1 : Anti-Follistatin antibody (ab58920) at 1 µg/ml
    Lane 2 : Anti-Follistatin antibody (ab58920) at 2.5 µg/ml
    Lane 3 : Anti-Follistatin antibody (ab58920) at 5 µg/ml

    All lanes : Cell lysate prepared from human Jurkat cells

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 35 kDa

References

This product has been referenced in:
  • Jones AE  et al. Wnt/ß-catenin controls follistatin signalling to regulate satellite cell myogenic potential. Skelet Muscle 5:14 (2015). Read more (PubMed: 25949788) »
  • Gao MH  et al. beta(1)-Adrenergic receptor vs adenylyl cyclase 6 expression in cardiac myocytes: differences in transgene localization and intracellular signaling. Cell Signal 22:584-9 (2010). Read more (PubMed: 19932173) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Sheep Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine Gels)
Loading amount
70 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. John Odhiambo

Verified customer

Submitted Oct 22 2015

Answer

DISCOUNT CODE:xxxxxxx
Expiration date: 20/03/2013
Value: CAD356.00
I am very pleased to offer you to test ab58920 in zebrafish. Because the only tested application for this product is WB it would be imprtant to tests the antibody on zebrafish in WB. This code will give you CAD356.00 off your next order before the expiration date. To redeem this offer, please submit an Abreview for zebrafish in WB and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Question
Answer

Thank you for kindly confirming these details. I am sorry this vial of antibody has not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab58920 has been added to your order number [BO] ASP/12032206. This has Abcam order reference 1095220.

I would like to reassure you that this free of charge replacement vial is also covered by our Abpromise guarantee. Should the customer still be experiencing difficulties with the new vial, or if you have any further questions, please do not hesitate to let me know.

Thank you for your help and cooperation with this case. Please do not hesitate to contact me if you need anything further.

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Answer

Thank you for your message and for providing this further information.

I can confirm, as previously discussed, that the recommended concentration for Western blot using this antibody is 2.5 µg/ml (1:400) as stated on the datasheet. This is a recommendation only and may require some optimization.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or credit note in compensation.

I look forward to hearing from you with details of how you would like to proceed.

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Answer

Sorry for the misunderstanding. Both antibodies have been developed to recognise specifically the follistatin. I would say that both products are equally specific to the target. The polyclonal antibody was raised against a 49 amino acid long peptide. The bulk of polyclonal antibodies would therefore bind at different places along this peptide. On the other hand, the monoclonal would bind to one particular fragment of the protein. Unfortunately the epitope for ab89515 has not been mapped. It can be anywhere along the follistatin since the immunogen used is the full length Human protein. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic mouse head E16)
Specification
Embryonic mouse head E16
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 (0.1%)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Dr. Gaskon Ibarretxe

Verified customer

Submitted Feb 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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