Product nameAnti-Follistatin antibody [EPR10903] - BSA and Azide free
See all Follistatin primary antibodies
DescriptionRabbit monoclonal [EPR10903] to Follistatin - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment within Human Follistatin. The exact sequence is proprietary.
Database link: P19883
- WB: HepG2 and C6 cell lysates; human heart, rat heart and kidney tissue lysate.
ab249335 is the carrier-free version of ab157471 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab249335 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab249335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.|
FunctionBinds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
Tissue specificityIsoform 1 is the predominant isoform in serum but is undetectable in follicular fluid.
Sequence similaritiesContains 3 follistatin-like domains.
Contains 3 Kazal-like domains.
Contains 1 TB (TGF-beta binding) domain.
- Information by UniProt
- Activin binding protein antibody
- Activin-binding protein antibody
- Follistatin antibody
All lanes : Anti-Follistatin antibody [EPR10903] (ab157471) at 1/1000 dilution (Purified)
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : Human heart lysates
Lane 3 : Rat heart lysates
Lane 4 : Rat kidney lysates
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
We are unsure how to define the extra bands.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157471).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab249335 has not yet been referenced specifically in any publications.