Anti-Forkhead box protein O/dFOXO antibody - N-terminal (ab195977)
Key features and details
- Rabbit polyclonal to Forkhead box protein O/dFOXO - N-terminal
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-Forkhead box protein O/dFOXO antibody - N-terminal -
Description
Rabbit polyclonal to Forkhead box protein O/dFOXO - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
Synthetic peptide corresponding to Drosophila melanogaster Forkhead box protein O/dFOXO (N terminal).
Database link: Q95V55 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Lyophilized:Reconstitute with 100 uL distilled water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 1.21% Tris buffer, 0.75% Glycine, 2% Sucrose -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
Target
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Function
Transcription factor involved in the regulation of the insulin signaling pathway. Consistently activates both the downstream target Thor\d4EBP and the feedback control target InR. Involved in negative regulation of the cell cycle, modulating cell growth and proliferation. In response to cellular stresses, such as nutrient deprivation or increased levels of reactive oxygen species, foxo is activated and inhibits growth through the action of target genes such as Thor. Foxo activated in the adult fat body can regulate lifespan in adults; an insulin peptide itself may function as one secondary messenger of insulin-regulated aging. Also regulates Lip4, homolog of human acid lipases, thereby acting as a key modulator of lipid metabolism by insulin signaling and integrates insulin responses to glucose and lipid homeostasis. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. - Information by UniProt
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Database links
- Entrez Gene: 41709 Drosophila melanogaster
- SwissProt: Q95V55 Drosophila melanogaster
- Unigene: 3957 Drosophila melanogaster
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Alternative names
- dFOXO antibody
- Forkhead box protein O antibody
- foxo antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab195977 has been referenced in 6 publications.
- Nam S et al. Tctp regulates the level and localization of Foxo for cell growth in Drosophila. Cell Death Discov 8:146 (2022). PubMed: 35361773
- Bhattacharjee S et al. PP2A phosphatase regulates cell-type specific cytoskeletal organization to drive dendrite diversity. Front Mol Neurosci 15:926567 (2022). PubMed: 36452406
- Zhang F et al. Mitochondria regulate intestinal stem cell proliferation and epithelial homeostasis through FOXO. Mol Biol Cell 31:1538-1549 (2020). PubMed: 32374658
- Wang M et al. 20(S)-ginsenoside Rg3 promotes myoblast differentiation and protects against myotube atrophy via regulation of the Akt/mTOR/FoxO3 pathway. Biochem Pharmacol 180:114145 (2020). PubMed: 32653593
- Zhai Y et al. Quantitative Proteomic and Transcriptomic Analyses of Metabolic Regulation of Adult Reproductive Diapause in Drosophila suzukii (Diptera: Drosophilidae) Females. Front Physiol 10:344 (2019). PubMed: 31019467
- Guo P et al. Effect and mechanism of fuzhisan and donepezil on the sirtuin 1 pathway and amyloid precursor protein metabolism in PC12 cells. Mol Med Rep 13:3539-46 (2016). PubMed: 26936536