Overview

  • Product name

    Formaldehyde Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Saliva, Urine, Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Sensitivity

    > 2 µM
  • Assay time

    1h 00m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Formaldehyde Assay Kit (Fluorometric) (ab196997) provides a simple, sensitive and high-throughput adaptable assay that detects biologically relevant concentrations of formaldehyde (HCHO) in various fluids and tissues. The assay relies on the oxidation of formaldehyde to produce a stable fluorescent signal, which is directly proportional to the amount of formaldehyde found in samples.

    The kit can detect less than 2 µM of formaldehyde in a variety of samples.

  • Notes

    Formaldehyde (HCHO) is the simplest aldehyde and one of the most often used organic compounds in industrial processes due to its high reactivity with a variety of chemicals. Endogenous formaldehyde can be produced in organisms via metabolic processes while exogenous formaldehyde can be absorbed after oral, dermal or inhalation exposure. formaldehyde is highly toxic due to its capacity to covalently bind to macromolecules, such as DNA, and is well known for its neurotoxicological effects. Exposure to relatively high levels of formaldehyde is believed to cause leukemia, nose and nasopharyngeal cancer, etc.; however the epidemiological evidence is unclear. It can also affect memory and learning capacity.

  • Platform

    Microplate reader

Properties

Images

  • Typical Formaldehyde (HCHO) standard calibration curve using fluorometric reading.

  • Measurement of Formaldehyde (HCHO) concentration in human urine from non-diabetic and diabetic donors. Samples were deproteinized using 10 kD Spin Column (ab93349) and diluted 10X. Diluted samples (20 µL) were spiked with known amount of Formaldehyde (300 pmol). Sample preparation and assay was performed following kit protocol.

Protocols

References

ab196997 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for getting in touch.

The ab196997 Formaldehyde Assay Kit (Fluorometric) detects biologically relevant concentrations of HCHO in various fluids and tissues including serum, plasma, saliva, urine and Animal tissues & cells as stated on the datasheet.  It has not been tested using ethanol based samples. It’s a possibility that there might be some interference from the ethanol based samples.

However, you could try testing these ethanol based samples (undiluted and couple of dilutions of the samples). The dilutions might dilute the ethanol amount in the samples and thus might be compatible with the assay.

I appreciate that using a kit that is untested for your sample type may pose some risk, so I would like to invite you to our AbTrial program. By participating in our AbTrial program you can use this product without financial risk by simply sharing your results in an Abreview.


How to take part in our AbTrial program:


1. Purchase the kit at regular price and reply to this email with your PO or order number.


2. I will generate a personal discount code equal to the value of the product.


3. Submit your results in an Abreview (including an image) at www.abcam.com/abreview. Enter your discount code in the additional notes section of the AbReview.


4. Once the AbReview is submitted, the discount code will become active.


5. Use your discount code to receive that value off your next purchase.


The Terms and Conditions of this offer can be found at www.abcam.com/abtrial.


I am happy to answer any further questions you have.

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