Formate Assay Kit (ab111748)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Overview
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Product name
Formate Assay Kit -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
Assay type
Quantitative -
Assay time
1h 00m -
Product overview
In Abcam's Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (λ = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.
Visit our FAQs page for tips and troubleshooting.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K653 Formate Colorimetric Assay Kit. K653-100 is the same size as the 100 test size of ab111748.
Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer II 1 x 25ml Developer Solution III 1 vial Enzyme Mix IX 1 vial Formate Standard 1 x 100µl -
Research areas
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab111748 has been referenced in 3 publications.
- Wang Y et al. Systematic evaluation of urinary formic acid as a new potential biomarker for Alzheimer's disease. Front Aging Neurosci 14:1046066 (2022). PubMed: 36533170
- Li G et al. p53 deficiency induces MTHFD2 transcription to promote cell proliferation and restrain DNA damage. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34244426
- Oizel K et al. Formate induces a metabolic switch in nucleotide and energy metabolism. Cell Death Dis 11:310 (2020). PubMed: 32366892