Anti-Fos B antibody [83B1138] (ab11959)
Key features and details
- Mouse monoclonal [83B1138] to Fos B
- Suitable for: Flow Cyt, IHC-Fr, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Fos B antibody [83B1138]
See all Fos B primary antibodies -
Description
Mouse monoclonal [83B1138] to Fos B -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
This antibody was raised against a synthetic peptide corresponding to human Fos B.
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Positive control
- HeLa whole-cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Affinity purified. -
Clonality
Monoclonal -
Clone number
83B1138 -
Isotype
IgG1 -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab11959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | 1/500 - 1/5000. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 40.6 kDa). |
Target
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Function
FosB interacts with Jun proteins enhancing their DNA binding activity. -
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2354 Human
- Entrez Gene: 14282 Mouse
- Entrez Gene: 100360880 Rat
- Omim: 164772 Human
- SwissProt: P53539 Human
- SwissProt: P13346 Mouse
- Unigene: 590958 Human
- Unigene: 248335 Mouse
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- DKFZp686C0818 antibody
see all
Images
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![Western blot - Anti-Fos B antibody [83B1138] (ab11959)](https://www.abcam.com/ps/products/11/ab11959/Images/ab11959-19209-anti-fos-b-antibody-83b1138-western-blot.jpg)
Western blot - Anti-Fos B antibody [83B1138] (ab11959)ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB. ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB. -
![Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959)](https://www.abcam.com/ps/products/11/ab11959/Images/ab11959-19207-anti-fos-b-antibody-83b1138-flow-cytometry.jpg)
Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959)Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959)](https://www.abcam.com/ps/products/11/ab11959/Images/ab11959-231193-anti-fos-b-antibody-83b1138-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959)Image courtesy of Dr. Karine Thibault, Université Paris Descartes, Franceab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.
Protocols
Datasheets and documents
References
ab11959 has been referenced in 22 publications.
- Noye Tuplin EW & Holahan MR Exploring time-dependent changes in conditioned place preference for food reward and associated changes in the nucleus accumbens. Behav Brain Res 361:14-25 (2019). PubMed: 30576721
- Qiao Y et al. Collateral Projections from the Lateral Parabrachial Nucleus to the Central Amygdaloid Nucleus and the Ventral Tegmental Area in the Rat. Anat Rec (Hoboken) 302:1178-1186 (2019). PubMed: 30332715
- Osacka J et al. Clozapine impact on FosB/?FosB expression in stress preconditioned rats: response to a novel stressor. Endocr Regul 53:83-92 (2019). IHC ; Rat . PubMed: 31517626
- Kovács LÁ et al. Corticotropin-Releasing Factor-Producing Cells in the Paraventricular Nucleus of the Hypothalamus and Extended Amygdala Show Age-Dependent FOS and FOSB/DeltaFOSB Immunoreactivity in Acute and Chronic Stress Models in the Rat. Front Aging Neurosci 11:274 (2019). PubMed: 31649527
- Ingallinesi M et al. Knock-Down of GPR88 in the Dorsal Striatum Alters the Response of Medium Spiny Neurons to the Loss of Dopamine Input and L-3-4-Dyhydroxyphenylalanine. Front Pharmacol 10:1233 (2019). PubMed: 31708775
