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  1. Link

    fos-b-antibody-83b1138-ab11959.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
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Anti-Fos B antibody [83B1138] (ab11959)

  • Datasheet
Reviews (3)Q&A (1)References (29)

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Western blot - Anti-Fos B antibody [83B1138] (ab11959)
  • Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959)
  • Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959)

Key features and details

  • Mouse monoclonal [83B1138] to Fos B
  • Suitable for: IHC-Fr, Flow Cyt, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Fos B antibody [83B1138]
    See all Fos B primary antibodies
  • Description

    Mouse monoclonal [83B1138] to Fos B
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Fos B.

  • Positive control

    • HeLa whole-cell extract.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Affinity purified.
  • Clonality

    Monoclonal
  • Clone number

    83B1138
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Assay kits

    • Fos B Transcription Factor Assay Kit (Colorimetric) (ab207197)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Fos B protein (ab152396)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 40.6 kDa).
Notes
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 40.6 kDa).

Target

  • Function

    FosB interacts with Jun proteins enhancing their DNA binding activity.
  • Sequence similarities

    Belongs to the bZIP family. Fos subfamily.
    Contains 1 bZIP domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P53539 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2354 Human
    • Entrez Gene: 14282 Mouse
    • Omim: 164772 Human
    • SwissProt: P53539 Human
    • SwissProt: P13346 Mouse
    • Unigene: 590958 Human
    • Unigene: 248335 Mouse
    • Alternative names

      • Activator protein 1 antibody
      • AP 1 antibody
      • DKFZp686C0818 antibody
      • FBJ murine osteosarcoma viral oncogene homolog B antibody
      • fosB antibody
      • FOSB_HUMAN antibody
      • G0/G1 switch regulatory protein 3 antibody
      • G0S3 antibody
      • GOS 3 antibody
      • GOS B antibody
      • GOS3 antibody
      • GOSB antibody
      • MGC42291 antibody
      • Oncogene FOS B antibody
      • Oncogene FOSB antibody
      • Protein fos B antibody
      • Protein fosB antibody
      see all

    Images

    • Western blot - Anti-Fos B antibody [83B1138] (ab11959)
      Western blot - Anti-Fos B antibody [83B1138] (ab11959)
      ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB. ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB.
    • Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959)
      Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959)
      Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959)
      Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959)Image courtesy of Dr. Karine Thibault, Université Paris Descartes, France

      ab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (29)

    Publishing research using ab11959? Please let us know so that we can cite the reference in this datasheet.

    ab11959 has been referenced in 29 publications.

    • Eagle AL  et al. Circuit-specific hippocampal ?FosB underlies resilience to stress-induced social avoidance. Nat Commun 11:4484 (2020). PubMed: 32901027
    • Sun Y  et al. Involvement of the Ventrolateral Periaqueductal Gray Matter-Central Medial Thalamic Nucleus-Basolateral Amygdala Pathway in Neuropathic Pain Regulation of Rats. Front Neuroanat 14:32 (2020). PubMed: 32792913
    • Ricke KM  et al. Neuronal Protein Tyrosine Phosphatase 1B Hastens Amyloid ß-Associated Alzheimer's Disease in Mice. J Neurosci 40:1581-1593 (2020). PubMed: 31915254
    • Cantacorps L  et al. Prenatal and postnatal alcohol exposure increases vulnerability to cocaine addiction in adult mice. Br J Pharmacol 177:1090-1105 (2020). PubMed: 31705540
    • Rentsch P  et al. Targeting the cannabinoid receptor CB2 in a mouse model of l-dopa induced dyskinesia. Neurobiol Dis 134:104646 (2020). PubMed: 31669673
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Fos B antibody [83B1138]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain slices)
    Permeabilization
    Yes - Triton 0.2%
    Specification
    Brain slices
    Blocking step
    PBS-Tween 0.05% + Goat Serum 10% + 0.3M Glycine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 21 2017

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Fos B antibody [83B1138]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - TritonX-100
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 15 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Fos B antibody [83B1138]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Karine Thibault

    Verified customer

    Submitted Aug 19 2014

    Question

    I need to know which part of the protein is regognize by the Ab? Is it specific for FosB or it will also recognize delta-FosB? Thank you

    Read More

    Abcam community

    Verified customer

    Asked on Aug 29 2007

    Answer

    Thank you for your enquiry. With this antibody we usually detect a second band of approximately 34 kDa which is delta-FosB. I hope this information will be useful to you. Should you require any other information, please do not hesitate to contact me. Good luck in your research.

    Read More

    Abcam Scientific Support

    Answered on Aug 29 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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