Anti-Fos B antibody [83B1138] (ab11959)
![Western blot - Anti-Fos B antibody [83B1138] (ab11959)](https://www.abcam.com/ps/products/11/ab11959/Images/ab11959-19209-anti-fos-b-antibody-83b1138-western-blot.jpg "Western blot - Anti-Fos B antibody [83B1138] (ab11959)")
Key features and details
- Mouse monoclonal [83B1138] to Fos B
- Suitable for: IHC-Fr, Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Fos B antibody [83B1138]
See all Fos B primary antibodies -
Description
Mouse monoclonal [83B1138] to Fos B -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Fos B.
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Positive control
- HeLa whole-cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Affinity purified. -
Clonality
Monoclonal -
Clone number
83B1138 -
Isotype
IgG1 -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 40.6 kDa).
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 40.6 kDa). |
Target
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Function
FosB interacts with Jun proteins enhancing their DNA binding activity. -
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2354 Human
- Entrez Gene: 14282 Mouse
- Omim: 164772 Human
- SwissProt: P53539 Human
- SwissProt: P13346 Mouse
- Unigene: 590958 Human
- Unigene: 248335 Mouse
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- DKFZp686C0818 antibody
see all
Images
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Western blot - Anti-Fos B antibody [83B1138] (ab11959)ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB. ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB.
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![Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959)](https://www.abcam.com/ps/products/11/ab11959/Images/ab11959-19207-anti-fos-b-antibody-83b1138-flow-cytometry.jpg)
Flow Cytometry - Anti-Fos B antibody [83B1138] (ab11959)Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959)](https://www.abcam.com/ps/products/11/ab11959/Images/ab11959-231193-anti-fos-b-antibody-83b1138-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Fos B antibody [83B1138] (ab11959)Image courtesy of Dr. Karine Thibault, Université Paris Descartes, Franceab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.
Protocols
Datasheets and documents
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Datasheet download
References (27)
ab11959 has been referenced in 27 publications.
- Eagle AL et al. Circuit-specific hippocampal ?FosB underlies resilience to stress-induced social avoidance. Nat Commun 11:4484 (2020). PubMed: 32901027
- Sun Y et al. Involvement of the Ventrolateral Periaqueductal Gray Matter-Central Medial Thalamic Nucleus-Basolateral Amygdala Pathway in Neuropathic Pain Regulation of Rats. Front Neuroanat 14:32 (2020). PubMed: 32792913
- Ricke KM et al. Neuronal Protein Tyrosine Phosphatase 1B Hastens Amyloid ß-Associated Alzheimer's Disease in Mice. J Neurosci 40:1581-1593 (2020). PubMed: 31915254
- Tyssowski KM et al. Firing Rate Homeostasis Can Occur in the Absence of Neuronal Activity-Regulated Transcription. J Neurosci 39:9885-9899 (2019). PubMed: 31672790
- Park JA et al. Increased Expression of FosB through Reactive Oxygen Species Accumulation Functions as Pro-Apoptotic Protein in Piperlongumine Treated MCF7 Breast Cancer Cells. Mol Cells 42:884-892 (2019). PubMed: 31735020
