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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
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RecombinantRabMAb

Recombinant Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)

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  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
  • Immunoprecipitation - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
  • Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15905] to Fos B - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

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Recombinant Human Fos B protein (ab152396)

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Overview

  • Product name

    Anti-Fos B antibody [EPR15905] - BSA and Azide free
    See all Fos B primary antibodies
  • Description

    Rabbit monoclonal [EPR15905] to Fos B - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    IP
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240288 is the carrier-free version of ab184938. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240288 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15905
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Alternative Versions

    • Anti-Fos B antibody [EPR15905] (ab184938)
    • Alexa Fluor® 555 Anti-Fos B antibody [EPR15905] (ab211854)
  • Assay kits

    • Fos B Transcription Factor Assay Kit (Colorimetric) (ab207197)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Immunohistochemistry kits

    • Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Fos B protein (ab152396)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Mouse
IP
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 38, 48 kDa (predicted molecular weight: 36 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 38, 48 kDa (predicted molecular weight: 36 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    FosB interacts with Jun proteins enhancing their DNA binding activity.
  • Sequence similarities

    Belongs to the bZIP family. Fos subfamily.
    Contains 1 bZIP domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P53539 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2354 Human
    • Entrez Gene: 14282 Mouse
    • Entrez Gene: 100360880 Rat
    • Omim: 164772 Human
    • SwissProt: P53539 Human
    • SwissProt: P13346 Mouse
    • Unigene: 590958 Human
    • Unigene: 248335 Mouse
    • Alternative names

      • Activator protein 1 antibody
      • AP 1 antibody
      • DKFZp686C0818 antibody
      • FBJ murine osteosarcoma viral oncogene homolog B antibody
      • fosB antibody
      • FOSB_HUMAN antibody
      • G0/G1 switch regulatory protein 3 antibody
      • G0S3 antibody
      • GOS 3 antibody
      • GOS B antibody
      • GOS3 antibody
      • GOSB antibody
      • MGC42291 antibody
      • Oncogene FOS B antibody
      • Oncogene FOSB antibody
      • Protein fos B antibody
      • Protein fosB antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)

      Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse hippocampus is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184938).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
      Immunocytochemistry/ Immunofluorescence - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, untreated or treated with 12-o-tetradecanoyl phorbol 13-acetate (TPA) (200nM) for 4h, labeling Fos B with ab184938 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing nuclear staining on HeLa cells. The expression was increaed after treatment with 12-o-tetradecanoyl phorbol 13-acetate (TPA) (200nM) for 4h.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab184938 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184938).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)

      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse kidney is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184938).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)

      Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat hippocampus is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184938).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)

      Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on rat kidney is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184938).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
      Immunoprecipitation - Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)

      Fos B was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr using ab184938 at 1/50 dilution.

      Western blot was performed from the immunoprecipitate using ab184938 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr, 10 µg (Input).

      Lane 2: ab184938 IP in HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184938 in HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184938).

    • Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)
      Anti-Fos B antibody [EPR15905] - BSA and Azide free (ab240288)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab240288? Please let us know so that we can cite the reference in this datasheet.

    ab240288 has not yet been referenced specifically in any publications.

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