Recombinant Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23489-90] to Fos B - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Fos B antibody [EPR23489-90] - BSA and Azide free
See all Fos B primary antibodies -
Description
Rabbit monoclonal [EPR23489-90] to Fos B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB & IP: HeLa whole cell lysate, serum starved o/n, then treated with 200 nM 12-o-tetradecanoyl phorbol 13-acetate for 4 hrs. IHC-P: Human breast cancer and hippocampus tissue. ICC/IF: HeLa cells, starved o/n, then treated with 200 nM 12-o-tetradecanoyl phorbol 13-acetate for 4 hrs. Flow Cyt (intra): HeLa cells, serum starved o/n, then treated with 200 nM 12-o-tetradecanoyl phorbol 13-acetate for 4 hrs.
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General notes
ab269953 is the carrier-free version of ab252237.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23489-90 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
Target
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Function
FosB interacts with Jun proteins enhancing their DNA binding activity. -
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2354 Human
- Omim: 164772 Human
- SwissProt: P53539 Human
- Unigene: 590958 Human
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- DKFZp686C0818 antibody
see all
Images
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) was serum starved for 16 hours, then treated with 200nM 12-O-Tetradecanoylphorbol-13-acetate (TPA) for 4 h (Red) / Untreated control (Green) cells labeling Fos B with ab252237 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252237).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Fos B with ab252237 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells treated with starvation 16 hours, then 12-O-Tetradecanoylphorbol-13-acetate (200nM) for 4 hours. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252237).
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Fos B was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) serum starved overnight, then treated with 200 nM TPA for 4 hours, whole cell lysate 10 µg with ab252237 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab252237 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: HeLa serum starved overnight, then treated with 200 nM TPA for 4 hours, whole cell lysate 10 µg.
Lane 2: ab252237 IP in HeLa serum starved overnight, then treated with 200 nM TPA for 4 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252237 in HeLa serum starved overnight, then treated with 200 nM TPA for 4 hours, whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252237).
The band around 37kDa is caused by splicing isoform.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Fos B with ab252237 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive nuclear staining in cancer cells of human breast cancer is observed (PMID: 12602926). The section was incubated with ab252237 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252237).
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Immunohistochemical analysis of paraffin-embedded human hippocampus tissue labeling Fos B with ab252237 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive nuclear staining in neurons of human hippocampus is observed (PMID: 29805976). The section was incubated with ab252237 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252237).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab269953 has not yet been referenced specifically in any publications.