Overview

  • Product name

  • Description

    Rabbit polyclonal to Fox2 / RBM9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Fox2/ RBM9 aa 8-110.
    Database link: O43251

  • Positive control

    • IHC-P: Human placenta and glioma tissues. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab236799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500.
ICC/IF 1/50 - 1/200.

Target

  • Function

    RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • HRNBP2 antibody
    • Fox 1 homologue antibody
    • Fox-1 homolog B antibody
    • FOX2 antibody
    • FXH antibody
    • Hexaribonucleotide-binding protein 2 antibody
    • HNRBP2 antibody
    • RBFOX2 antibody
    • Repressor of tamoxifen transcriptional activity antibody
    • RFOX2_HUMAN antibody
    • RNA binding motif protein 9 antibody
    • RNA binding protein 9 antibody
    • RNA binding protein fox-1 homolog 2 antibody
    • RNA-binding motif protein 9 antibody
    • RNA-binding protein 9 antibody
    • RTA antibody
    see all

Images

  • Paraffin-embedded human placenta tissue stained for Fox2 / RBM9 using ab236799 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human glioma tissue stained for Fox2 / RBM9 using ab236799 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • 4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Fox2 / RBM9 (green) using ab236799 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

References

ab236799 has not yet been referenced specifically in any publications.

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