Anti-FOXA1 antibody (ab5089)

Goat polyclonal FOXA1 antibody. Validated in WB, IHC and tested in Mouse, Human. Cited in 59 publication(s). Independently reviewed in 2 review(s). Immunogen corresponding to synthetic peptide.

Reviews (2)Q&A (8)References (60)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab5089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 20018680
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49.1 kDa).Can be blocked with Human FOXA1 peptide (ab23074).

An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide.

Target

  • Function

    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

  • Tissue specificity

    Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.

  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • forkhead box A1 antibody
    • Forkhead box protein A1 antibody
    • FOX A1 antibody
    • FOXA1 antibody
    • FOXA1_HUMAN antibody
    • hepatocyte nuclear factor 3 alpha antibody
    • Hepatocyte nuclear factor 3-alpha antibody
    • HNF 3A antibody
    • HNF-3-alpha antibody
    • HNF-3A antibody
    • HNF3A antibody
    • MGC33105 antibody
    • TCF 3A antibody
    • TCF-3A antibody
    • TCF3A antibody
    • Transcription factor 3A antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody (ab5089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody (ab5089)
    ab5089 at 5 µg/ml staining FOXA1 in Human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Western blot - Anti-FOXA1 antibody (ab5089)
    Western blot - Anti-FOXA1 antibody (ab5089)
    Anti-FOXA1 antibody (ab5089) at 0.3 µg/ml + HepG2 at 35 µg

    Predicted band size: 49.1 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.



    An additional band of unknown identity was also consistently observed at 37kDa.

References

This product has been referenced in:

  • Grimm SA  et al. DNA methylation in mice is influenced by genetics as well as sex and life experience. Nat Commun 10:305 (2019). Read more (PubMed: 30659182) »
  • Glont SE  et al. Comprehensive Genomic Analysis Reveals that the Pioneering Function of FOXA1 Is Independent of Hormonal Signaling. Cell Rep 26:2558-2565.e3 (2019). Read more (PubMed: 30840881) »
See all 60 Publications for this product

Publishing research using ab5089? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 10 Abreviews or Q&A

Question

Product code: 5089
Inquiry: Please can you let me know if the following lots are available for FOXA1 antibody (cat. no. ab5089): 887289, 887288 or 586788. If these lots are not available, please can you let know which lot numbers are available.

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Answer

Thank you for contacting us.

The lot numbers you have kindly asked are sold out.

The following lot numbers are currently in stock, GR115830-1 and GR115838-1.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Inquiry: Hi, I am looking for a Foxa1 antibody to use on PFA fixed then frozen tissue. I see that ab23738 has been tested using these conditions, but I need a product that is not raised in rabbit. Has ab5089 ever been tested on Fixed-Frozen tissue? Would you be willing to send a test sample so that I can try it in this application?

Read More
Answer

Thank you for contacting us.
Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
To our knowledge, ab5089 has not been tested in immunohistochemistry on frozen tissue sections (IHC-Fr). However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.
Simply follow these easy steps below to apply for our AbTrial Program:
1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

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Question

Sometime ago my lab technician contacted you about Abcam antibody (ab25752)that was not working properly and therefore you offered us a replacement of this product (see below mail conversation).

Now I want to order another Hnf3a antibody Anti-FOXA1 antibody - ChIP Grade (ab5089) as a replacement. Can you please place this replacement order for me?

Please let me know. Thanks in advance!

Read More
Answer

I am sorry this product did not perform as stated on the data sheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab23738 - Anti-FOXA1 antibody.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Question

Bonjour,
Suite à votre envoi d'un tube d'anticorps (anti FOXA1), nous avons testé
celui ci. Nous avons obtenu de meilleurs résultats qu'avec les précédents.
Comme convenu, vous pouvez donc nous envoyer le second tube.
En vous remerciant,
Cordialement,

Read More
Answer

Merci de votre émail.

Je suis heureuse d'apprendre que le nouveau lot fonctionne de nouveau comme attendu. Veuillez encore une fois accepter toutes nos excuses pour les problèmes que vous aviez eu avec les deux tubes précédents. Je me permets de vous transférer le numéro de commande du deuxième tube deremplacement gratuit de ce produit:xx. Il devrait arriver demain chez vous. Vous recevrez aussi prochainement un mail de confirmation comprenant les détails d'expédition.

N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Question

phone call:old lots in ChIP worked fine. this lot does not work well - has two vials. will send one vial to testas replacement with order xx (as same batch) - then if the vial works well, a second one.

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Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que les deux derniers tube de cet anticorps que vous avez reçu ne fonctionnent pas comme attendus. Comme discuté au téléphone, comme nous avons que le même lot en stock, je vous envoie une unité gratuite de remplacement. Si cette unité vous donne des bons résultats, nous allons vous envoyer un deuxième tube gratuitement. Voicile numéro de commande de remplacement gratuit de ce produit : xx. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

J'espère que le nouveau tube va donner les résultats attendus. Veuillez ne pas hésiter à nous recontacter aussi si vous avec des questions. Je me réjouis d'avoir de vous nouvelles.

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Question

Inquiry: Dear whom it may concern. We wish to conduct an immunohistochemical analysis on paraffin embedded liver tissue from pig. We wish to verify the presence of FOXA1 (Forkhead box A1). Do you have an antibody binding to this protein that is specific for porcine liver tissue? And is it possible to send us a small sample for testing of the antibody? Thank you in advance for considering this inquiry. Best regards

Read More
Answer

Thank you for contacting us.

We have ab5089 and ab115279 antibodies that are predicted to react based on sequence homology between human and Porcine. These are the best suitable antibodies we can offer and are eligible for 100% Abreview discount programme.

Clickthe linkfor more info:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Western blot abreview for Anti-FOXA1 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (HepG2)
Gel Running Conditions
Reduced Denaturing (4-12% NuPAGE)
Loading amount
1e+006 cells
Specification
HepG2
Blocking step
Blocking agent - RPN2125V (GE) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Mr. Mehdi Motallebipour

Verified customer

Submitted May 16 2008

Question

Is this antibody specific to FoxA1 or does it recognize the related family member FoxA2 (that contains part of the immunogen sequence) ? Thanks

Read More
Answer

Thank you for your enquiry regarding the specificity of our antibody raised against FOXA1 (ab5089). There is 72% sequence homology between the FOXA1 synthetic peptide immunogen used to raise this antibody and FOXA2. This means there is a small chance that it will also detect FOXA2, perhaps giving some weak staining from cross-reactivity. I am sorry that to my knowledge there is no experimental data available on this. I hope this helps. Please get back to me if you have any more questions and I will be happy to help.

Read More

Question

I understand FoxA1 has 3 isoforms (alpha, beta and gamma). Which isoform does this antibody recognize? All 3?

Read More
Answer

Thank you for your enquiry and patience. I enquired with the originator of this antibody and we cannot find references to FOXA1 isoforms in Entrez Gene or SwissProt. The sequence of the immunogen used to generate this antibody is as follows: GVYSRPVLNTS, corresponding to amino acids 463-473 of Human FOX A1. So if you have the sequences of the isoforms, you can compare the immunogen sequence with them. Please contact us again if you have any additional questions.

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ChIP abreview for Anti-FOXA1 antibody

 Excellent
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - whole cell (MCF-7 breast cancer cells)
Specification
MCF-7 breast cancer cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
The negative control is an anti-FOXA1 antibody from another vendor.
Read More

Abcam user community

Verified customer

Submitted Oct 04 2005

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