Overview

  • Product name

    Anti-FOXA1 antibody - ChIP Grade
    See all FOXA1 primary antibodies
  • Description

    Rabbit polyclonal to FOXA1 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ChIP/Chip, ChIP, EMSAmore details
    Unsuitable for: ICC/IF,IHC-FoFr or IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human FOXA1 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P55317
    (Peptide available as ab25752)

  • Positive control

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.7 - 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 49 kDa).
ChIP/Chip Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF,IHC-FoFr or IHC-P.
  • Target

    • Function

      Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
    • Tissue specificity

      Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
    • Sequence similarities

      Contains 1 fork-head DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • forkhead box A1 antibody
      • Forkhead box protein A1 antibody
      • FOX A1 antibody
      • FOXA1 antibody
      • FOXA1_HUMAN antibody
      • hepatocyte nuclear factor 3 alpha antibody
      • Hepatocyte nuclear factor 3-alpha antibody
      • HNF 3A antibody
      • HNF-3-alpha antibody
      • HNF-3A antibody
      • HNF3A antibody
      • MGC33105 antibody
      • TCF 3A antibody
      • TCF-3A antibody
      • TCF3A antibody
      • Transcription factor 3A antibody
      see all

    Images

    • All lanes : Anti-FOXA1 antibody - ChIP Grade (ab23738) at 0.7 µg/ml

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : FOXA1 knockout HAP1 whole cell lysate
      Lane 3 : HepG2 whole cell lysate
      Lane 4 : A549 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 49 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab23738 observed at 49 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab23738 was shown to recognize FOXA1 in wild-type HAP1 cells as signal was lost at the expected MW in FOXA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FOXA1 knockout samples were subjected to SDS-PAGE. Ab23738 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.7 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-FOXA1 antibody - ChIP Grade (ab23738) at 1/1000 dilution

      Lane 1 : Lysate prepared from human LNCaP cells
      Lane 2 : Lysate prepared from MCF7 cells
      Lane 3 : Lysate prepared from human LNCaP cells with ethanol as vehicle control
      Lane 4 : Lysate prepared from human LNCaP cells with 100 nM DHT

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 49 kDa
      Observed band size: 51 kDa
      why is the actual band size different from the predicted?


      Exposure time: 2 seconds

      See Abreview

    • Anti-FOXA1 antibody - ChIP Grade (ab23738) at 1 µg/ml + Human heart tissue lysate at 20 µg

      Secondary
      Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Predicted band size: 49 kDa
      Observed band size: 51 kDa why is the actual band size different from the predicted?
      Additional bands at: 31 kDa (possible cleavage fragment), 31 kDa (possible cross reactivity)

    • Anti-FOXA1 antibody - ChIP Grade (ab23738) at 1 µg/ml + Recombinant Human FOXA1 protein (ab98301) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 49 kDa


      Exposure time: 10 seconds

    References

    This product has been referenced in:

    • Yee CH  et al. Maintenance of the bladder cancer precursor urothelial hyperplasia requires FOXA1 and persistent expression of oncogenic HRAS. Sci Rep 9:270 (2019). Read more (PubMed: 30670749) »
    • Glont SE  et al. Comprehensive Genomic Analysis Reveals that the Pioneering Function of FOXA1 Is Independent of Hormonal Signaling. Cell Rep 26:2558-2565.e3 (2019). Read more (PubMed: 30840881) »
    See all 92 Publications for this product

    Customer reviews and Q&As

    1-10 of 24 Abreviews or Q&A

    Answer

    Thank you for contacting us. As requested I have added ab25752, FOXA1 peptide,to be provided free of charge for ***. This should arrive with your next shippment. Please let me know if you have any questions or if thereis anything else which we might be able to do for you or ****.

    Read More
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (airway cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    airway cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

    Dr. James Browne

    Verified customer

    Submitted Feb 08 2019

    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (airway cells)
    Specification
    airway cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde

    Dr. James Browne

    Verified customer

    Submitted Feb 07 2019

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7 (breast cancer), HepG2 (Liver cancer))
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    15 µg
    Specification
    MCF7 (breast cancer), HepG2 (Liver cancer)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 30 2018

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MDA-MB-231 cell)
    Permeabilization
    Yes - Tween-20
    Specification
    MDA-MB-231 cell
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Acetone

    Abcam user community

    Verified customer

    Submitted Oct 29 2018

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human breast cancer tissue (form MDA-MB-231 Xenogr)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
    Permeabilization
    Yes - Tween-20
    Specification
    Human breast cancer tissue (form MDA-MB-231 Xenogr
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 29 2018

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS/EDTA Buffer pH 9,0
    Sample
    Human Tissue sections (Prostate)
    Specification
    Prostate
    Permeabilization
    Yes - Washbuffer from Dako with Tween
    Fixative
    Paraformaldehyde

    Mr. Rudolf Jung

    Verified customer

    Submitted Aug 21 2014

    Answer

    I am sorry this product did not perform as stated on the data sheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab23738 - Anti-FOXA1 antibody.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    15 µg
    Specification
    HeLa
    Treatment
    non-targeting siRNA
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Oct 19 2012

    Answer

    Thank you for these additional details.

    The image and protocol provided make it clear to me that this product is not recognizing HNF3a and I thank you for providing them. I am sorry that this product has not performed as stated on the datasheet and would like to offer a replacement or refund provided as you had contacted us within the six month Abpromise guarantee period.

    I look forward to hearing how you would like to proceed. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    1-10 of 24 Abreviews or Q&A

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