• Product name
    Anti-FOXA1 antibody - ChIP Grade
    See all FOXA1 primary antibodies
  • Description
    Rabbit polyclonal to FOXA1 - ChIP Grade
  • Host species
  • Tested applications
    Suitable for: IHC-FoFr, WB, IHC-P, ChIP/Chip, ChIP, EMSA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human FOXA1 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P55317
    (Peptide available as ab25752)

  • Positive control



Our Abpromise guarantee covers the use of ab23738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 0.7 - 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 49 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP/Chip Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
  • Tissue specificity
    Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • forkhead box A1 antibody
    • Forkhead box protein A1 antibody
    • FOX A1 antibody
    • FOXA1 antibody
    • FOXA1_HUMAN antibody
    • hepatocyte nuclear factor 3 alpha antibody
    • Hepatocyte nuclear factor 3-alpha antibody
    • HNF 3A antibody
    • HNF-3-alpha antibody
    • HNF-3A antibody
    • HNF3A antibody
    • MGC33105 antibody
    • TCF 3A antibody
    • TCF-3A antibody
    • TCF3A antibody
    • Transcription factor 3A antibody
    see all


  • All lanes : Anti-FOXA1 antibody - ChIP Grade (ab23738) at 1/1000 dilution

    Lane 1 : Lysate prepared from human LNCaP cells
    Lane 2 : Lysate prepared from MCF7 cells
    Lane 3 : Lysate prepared from human LNCaP cells with ethanol as vehicle control
    Lane 4 : Lysate prepared from human LNCaP cells with 100 nM DHT

    Lysates/proteins at 20 µg per lane.

    All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 51 kDa (why is the actual band size different from the predicted?)

    Exposure time: 2 seconds

    See Abreview

  • ab23738 stained in MCF7 cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23738 at 5µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • (Image courtesy of Human Protein Atlas) ab23738 staining FOXA1 in Human prostate. Paraffin embedded human prostate tissue was incubated with ab23738 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23738 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • Anti-FOXA1 antibody - ChIP Grade (ab23738) at 1 µg/ml + Human heart tissue lysate at 20 µg

    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Predicted band size: 49 kDa
    Observed band size: 51 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 31 kDa (possible cleavage fragment), 31 kDa (possible cross reactivity)


This product has been referenced in:
  • Wang K  et al. Clinical significance and prognostic value of Forkhead box A1 expression in human epithelial ovarian cancer. Oncol Lett 15:4457-4462 (2018). Read more (PubMed: 29541214) »
  • Hoffman JA  et al. BRG1 governs glucocorticoid receptor interactions with chromatin and pioneer factors across the genome. Elife 7:N/A (2018). Read more (PubMed: 29792595) »

See all 71 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. As requested I have added ab25752, FOXA1 peptide,to be provided free of charge for ***. This should arrive with your next shippment. Please let me know if you have any questions or if thereis anything else which we mi...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS/EDTA Buffer pH 9,0
Human Tissue sections (Prostate)
Yes - Washbuffer from Dako with Tween

Mr. Rudolf Jung

Verified customer

Submitted Aug 21 2014

I am sorry this product did not perform as stated on the data sheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab23738 - Anti-FOXA1 antibody.

To check the status of the order please...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (HeLa)
Loading amount
15 µg
non-targeting siRNA
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 19 2012

Thank you for these additional details.

The image and protocol provided make it clear to me that this product is not recognizing HNF3a and I thank you for providing them. I am sorry that this product has not performed as stated on the data...

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Thank you for contacting us.

Would you be able to send me an image of the blocking peptide experiments and the protocol details of that experiment?

I look forward to your reply. Please do not hesitate to contact us if you have a...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team...

Read More

Thank you for contacting us.

All the products comes with 6 month guarantee period if used in applications and species as listed on the datasheet. Please store the product at correct temperature asap and use it; if it does not work then do not ...

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Thank you for your reply. I have reviewed your protocol and would like to send to you the blocking peptide for ab23738 so that you may perform an immunogen blocking experiment. I recommend this as you have already optimized the blot. This experiment...

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Thank you for your response.

I would be very glad to help you. In order to do this, could you please provide me with the Abcam order confirmation number which you had recieved when ordering ab23738. If you do not have this information I ma...

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