Anti-FOXA1 antibody (ab5089)
Key features and details
- Goat polyclonal to FOXA1
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-FOXA1 antibody
See all FOXA1 primary antibodies -
Description
Goat polyclonal to FOXA1 -
Host species
Goat -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Xenopus laevis, Zebrafish -
Immunogen
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Positive control
- WB: HepG2 and MCF7 nuclear cell lysates; Mouse liver lysate. ICC: MCF7 and U2OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49.1 kDa).
A 1 hour primary incubation at room temperature is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. |
ICC |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49.1 kDa). A 1 hour primary incubation at room temperature is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. |
ICC
Use a concentration of 10 µg/ml. |
Target
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Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis. -
Tissue specificity
Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3169 Human
- Entrez Gene: 15375 Mouse
- Entrez Gene: 25098 Rat
- Omim: 602294 Human
- SwissProt: P55317 Human
- SwissProt: P35582 Mouse
- SwissProt: P23512 Rat
- Unigene: 163484 Human
see all -
Alternative names
- forkhead box A1 antibody
- Forkhead box protein A1 antibody
- FOX A1 antibody
see all
Images
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Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling FOXA1 with ab5089 at 10 ug/ml (1h). Image showing nuclear staining in MCF7 cells. An Alexa Fluor 488® secondary antibody was used at 2 ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Secondary antibody only control: Unimmunized goat IgG (10 ug/ml) followed by an Alexa Fluor 488® secondary antibody at 2ug/ml.
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Lane 1 : Anti-FOXA1 antibody (ab5089) at 0.1 µg/ml
Lane 2 : Anti-FOXA1 antibody (ab5089) at 0.3 µg/ml
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) nuclear cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) nuclear cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 49.1 kDaWestern blot analysis labeling FOXA1 with ab5089.
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Anti-FOXA1 antibody (ab5089) at 0.1 µg/ml + Mouse liver lysate at 35 µg
Predicted band size: 49.1 kDaWestern blot labeling FOXA1 with ab5089 at 0.1 ug/ml.
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Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% TritonX-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling FOXA1 with ab5089 at 10 ug/ml (1h). Image showing nuclear staining in U-2 OS cells. An Alexa Fluor 488® secondary antibody was used at 2 ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Secondary antibody only control: Unimmunized goat IgG (10 ug/ml) followed by an Alexa Fluor 488® secondary antibody at 2ug/ml.
Datasheets and documents
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Datasheet download
References (80)
ab5089 has been referenced in 80 publications.
- Wang Y et al. Expression of FOXA1 Is Associated with the Tumor-Infiltrating M2 Macrophage, Cytotoxic T Lymphocyte, and Effect of Chemotherapy in Bladder Cancer. Urol Int 107:58-63 (2023). PubMed: 34706362
- Warrick JI et al. FOXA1 repression drives lineage plasticity and immune heterogeneity in bladder cancers with squamous differentiation. Nat Commun 13:6575 (2022). PubMed: 36323682
- Geusz RJ et al. Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors. Nat Commun 12:6636 (2021). PubMed: 34789735
- Severson TM et al. Epigenetic and transcriptional analysis reveals a core transcriptional program conserved in clonal prostate cancer metastases. Mol Oncol 15:1942-1955 (2021). PubMed: 33576154
- Li Y et al. Differential gene expression identifies a transcriptional regulatory network involving ER-alpha and PITX1 in invasive epithelial ovarian cancer. BMC Cancer 21:768 (2021). PubMed: 34215221