Overview

  • Product name
    Anti-FOXA1 antibody [EPR10881-14]
    See all FOXA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10881-14] to FOXA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: IP or WB
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human FOXA1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P55317

  • Positive control
    • Human breast carcinoma and prostate tissues; HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab173287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2000.
ICC/IF 1/1000.
  • Application notes
    Is unsuitable for IP or WB.
  • Target

    • Function
      Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
    • Tissue specificity
      Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
    • Sequence similarities
      Contains 1 fork-head DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • forkhead box A1 antibody
      • Forkhead box protein A1 antibody
      • FOX A1 antibody
      • FOXA1 antibody
      • FOXA1_HUMAN antibody
      • hepatocyte nuclear factor 3 alpha antibody
      • Hepatocyte nuclear factor 3-alpha antibody
      • HNF 3A antibody
      • HNF-3-alpha antibody
      • HNF-3A antibody
      • HNF3A antibody
      • MGC33105 antibody
      • TCF 3A antibody
      • TCF-3A antibody
      • TCF3A antibody
      • Transcription factor 3A antibody
      see all

    Images

    • IHC-Paraffin-embedded sections of Human prostate hyperplasia. 1:1000 dilution of ab173287 used. Counter stain = Hematoxylin.

    • Left image: Immunofluorescent staining of SW480 cells using ab173287(1:1000 dilution). 1:200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078). Right image: Immunofluorescent staining of SW480 cells with DAPI. Fixative = 4% paraformaldehyde.

    • IHC-Paraffin-embedded sections of Mouse colon. 1:1000 dilution of ab173287 used. Counter stain = Hematoxylin.  

    • Left image: Immunofluorescent staining of HepG2 cells using ab173287. 1:200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077). Right image: Immunofluorescent staining of HepG2 cells with DAPI. Fixative = 4% paraformaldehyde.

    • IHC-Paraffin-embedded sections of Human breast carcinoma. 1:1000 dilution of ab173287 used. Counter stain = hematoxylin.

    • ab173287 showing -ve staining in Human brain tissue. 1:1000 dilution of ab. Paraffin-embedded sections.

    References

    ab173287 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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