RecombinantRabMAb

Recombinant Anti-FOXA1 antibody [EPR10881-14] (ab173287)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
  • Anti-FOXA1 antibody [EPR10881-14] (ab173287)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10881-14] to FOXA1
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FOXA1 antibody [EPR10881-14]
    See all FOXA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10881-14] to FOXA1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment within Human FOXA1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P55317

  • Positive control

    • Human breast carcinoma and prostate tissues; HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab173287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/1000.
  • Application notes
    Is unsuitable for IP or WB.

    • Target

    • Function

      Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

    • Tissue specificity

      Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.

    • Sequence similarities

      Contains 1 fork-head DNA-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • forkhead box A1 antibody
        • Forkhead box protein A1 antibody
        • FOX A1 antibody
        • FOXA1 antibody
        • FOXA1_HUMAN antibody
        • hepatocyte nuclear factor 3 alpha antibody
        • Hepatocyte nuclear factor 3-alpha antibody
        • HNF 3A antibody
        • HNF-3-alpha antibody
        • HNF-3A antibody
        • HNF3A antibody
        • MGC33105 antibody
        • TCF 3A antibody
        • TCF-3A antibody
        • TCF3A antibody
        • Transcription factor 3A antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

        IHC-Paraffin-embedded sections of Human prostate hyperplasia. 1:1000 dilution of ab173287 used. Counter stain = Hematoxylin.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
        Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

        Left image: Immunofluorescent staining of SW480 cells using ab173287(1:1000 dilution). 1:200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078). Right image: Immunofluorescent staining of SW480 cells with DAPI. Fixative = 4% paraformaldehyde.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

        IHC-Paraffin-embedded sections of Mouse colon. 1:1000 dilution of ab173287 used. Counter stain = Hematoxylin.  

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
        Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

        Left image: Immunofluorescent staining of HepG2 cells using ab173287. 1:200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077). Right image: Immunofluorescent staining of HepG2 cells with DAPI. Fixative = 4% paraformaldehyde.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

        IHC-Paraffin-embedded sections of Human breast carcinoma. 1:1000 dilution of ab173287 used. Counter stain = hematoxylin.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

        ab173287 showing -ve staining in Human brain tissue. 1:1000 dilution of ab. Paraffin-embedded sections.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-FOXA1 antibody [EPR10881-14] (ab173287)
        Anti-FOXA1 antibody [EPR10881-14] (ab173287)

      References (1)

      Publishing research using ab173287? Please let us know so that we can cite the reference in this datasheet.

      ab173287 has been referenced in 1 publication.

      • Santos CP  et al. Urothelial organoids originating from Cd49fhigh mouse stem cells display Notch-dependent differentiation capacity. Nat Commun 10:4407 (2019). PubMed: 31562298

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