Key features and details
- Rabbit polyclonal to FOXA2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FOXA2 antibody
See all FOXA2 primary antibodies
DescriptionRabbit polyclonal to FOXA2
SpecificityAt least two isoforms of FOXA2 are known to exist; this antibody will detect both isoforms. ab191577 is predicted to not cross-react with other members of the FOXA protein family.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human FOXA2 (internal sequence). (16 amino acid peptide; NP_068556).
Database link: Q9Y261
- Human bladder tissue lysate; Human bladder tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 48 kDa).|
|IHC-P||Use a concentration of 20 µg/ml.|
FunctionTranscription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
modificationsPhosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
- Information by UniProt
- Forkhead box A2 antibody
- Forkhead box protein A2 antibody
- FOX A2 antibody
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling FOXA2 with Anti-FOXA2 antibody (ab191577) at 5μg/ml.
Anti-FOXA2 antibody (ab191577) at 1 µg/ml + Human bladder tissue lysate at 15 µg
Predicted band size: 48 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human bladder tissue labeling FOXA2 with ab191577 at 20 µg/ml.
ab191577 has not yet been referenced specifically in any publications.