Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXA2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human
    Predicted to work with: Rat, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human FOXA2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab25739.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: HepG2 Nuclear and Mouse Liver

Properties

Applications

Our Abpromise guarantee covers the use of ab23630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Forkhead box A2 antibody
    • Forkhead box protein A2 antibody
    • FOX A2 antibody
    • foxa2 antibody
    • FOXA2_HUMAN antibody
    • Hepatic nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3 antibody
    • Hepatocyte nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3-beta antibody
    • HNF 3B antibody
    • HNF-3-beta antibody
    • HNF-3B antibody
    • HNF3B antibody
    • MGC19807 antibody
    • TCF 3B antibody
    • TCF-3B antibody
    • TCF3B antibody
    • Transcription factor 3B antibody
    see all

Images

  • All lanes : Anti-FOXA2 antibody (ab23630) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 nuclear extract lysate (ab14660)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.

  • Anti-FOXA2 antibody (ab23630) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes
  • ICC/IF image of ab23630 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23630, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Nett IR  et al. Negative feedback via RSK modulates Erk-dependent progression from naïve pluripotency. EMBO Rep 19:N/A (2018). Read more (PubMed: 29895711) »
  • Liu C  et al. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells. Stem Cell Reports 11:43-57 (2018). Read more (PubMed: 30008328) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

DISCOUNT CODE: ABTRIAL-00000
Expiration date: 14. April 2013
Value: £ 234


I am very pleased to hear you would like to accept our offer and test ab23630 in IHC-Fr. This code will give you £ 234 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. It does not matter if the Abreview is positive or negative. Both is valuable data for us and other scientists.


For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the positive or negative Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More

Answer

Thank you very much for your interest our AbTrial program.


To our knowledge, ab23630 has not been tested in Cryosections (IHC-Fr). However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Read More

Answer

Thank you for contacting us.

What kind of application do you have in mind? We may be able to give you our AbTrial discount if it is a new application, but we would need to know what kind of application exactly (IHC-P, IHC-Fr e.g.) you want to perform.

We are looking forward to your reply.


Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Question
Answer

Vielen Dank für Ihren Anruf.

Wie versprochen habe ich alle unsere FOXA2-Antikörper darauf geprüft, welche Isoformen von FOXA2 sie erkennen. Leider sind alle Immunogene in Regionen lokalisiert, die in beiden Isoformen vorkommen (Details siehe jeweiliges Datenblatt), und daher detektieren die Antikörper immer beide Isoformen. Wir haben also leider keinen Antikörper erhältlich, der zwischen den Isoformen unterscheiden kann.

Es tut mir leid, dass ich keine besseren Nachrichten für Sie habe. Ich hoffe, diese Informationen helfen Ihnen dennoch weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (embryonic brain)
Specification
embryonic brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0,2% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C

Ms. Judit Kuechler

Verified customer

Submitted Jan 11 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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