Product nameAnti-FOXA2 antibody
See all FOXA2 primary antibodies
DescriptionRabbit polyclonal to FOXA2
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
3 synthetic peptides (human) conjugated to KLH
- Recombinant FOXA2 protein expressed in induced culture of E.coli (one shot Top10 competent cells).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituent: Whole serum
Concentration information loading...
Purification notesCrude serum, final bleed.
Our Abpromise guarantee covers the use of ab82827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 48 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
modificationsPhosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
- Information by UniProt
- Forkhead box A2 antibody
- Forkhead box protein A2 antibody
- FOX A2 antibody
All lanes : Anti-FOXA2 antibody (ab82827) at 1/1000 dilution
Lane 1 : Uninduced culture of E.coli (one shot Top10 competent cells)
Lane 2 : Induced culture of E.coli (one shot Top10 competent cells)
All lanes : Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Recombinant FOXA2 protein is expressed using pBAD-DEST49 plasmid. Molecular weight of tag is 14kDa.
ab82827 has not yet been referenced specifically in any publications.