• Product name
  • Description
    Rabbit polyclonal to FOXA2
  • Host species
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (human) conjugated to KLH

  • Positive control
    • Recombinant FOXA2 protein expressed in induced culture of E.coli (one shot Top10 competent cells).



Our Abpromise guarantee covers the use of ab82827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
    WB: 1/1000. Predicted molecular weight: 48 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
    • Sequence similarities
      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
    • Cellular localization
      Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Forkhead box A2 antibody
      • Forkhead box protein A2 antibody
      • FOX A2 antibody
      • foxa2 antibody
      • FOXA2_HUMAN antibody
      • Hepatic nuclear factor 3 beta antibody
      • Hepatocyte nuclear factor 3 antibody
      • Hepatocyte nuclear factor 3 beta antibody
      • Hepatocyte nuclear factor 3-beta antibody
      • HNF 3B antibody
      • HNF-3-beta antibody
      • HNF-3B antibody
      • HNF3B antibody
      • MGC19807 antibody
      • TCF 3B antibody
      • TCF-3B antibody
      • TCF3B antibody
      • Transcription factor 3B antibody
      see all


    • All lanes : Anti-FOXA2 antibody (ab82827) at 1/1000 dilution

      Lane 1 : Uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : Induced culture of E.coli (one shot Top10 competent cells)

      All lanes : Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 48 kDa
      Observed band size: 62 kDa (why is the actual band size different from the predicted?)

      Recombinant FOXA2 protein is expressed using pBAD-DEST49 plasmid. Molecular weight of tag is 14kDa.


    ab82827 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Vielen Dank für Ihren Anruf.

    Wie versprochen habe ich alle unsere FOXA2-Antikörper darauf geprüft, welche Isoformen von FOXA2 sie erkennen. Leider sind alle Immunogene in Regionen lokalisiert, die in beiden Isoformen vorkommen (Details siehe...

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