Key features and details
- Rabbit polyclonal to FOXA2 - C-terminal
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FOXA2 antibody - C-terminal
See all FOXA2 primary antibodies
DescriptionRabbit polyclonal to FOXA2 - C-terminal
Tested applicationsSuitable for: WB, IHC-Frmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken
Synthetic peptide within Human FOXA2 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: Q9Y261
- WB: HepG2 whole cell lysate. IHC-Fr: Mouse E10.5 embryonic neuron tube tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|IHC-Fr||1/100 - 1/1000.|
FunctionTranscription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
modificationsPhosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
- Information by UniProt
- Forkhead box A2 antibody
- Forkhead box protein A2 antibody
- FOX A2 antibody
Anti-FOXA2 antibody - C-terminal (ab228350) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 48 kDa
Frozen section of E10.5 mouse embryonic neuron tube tissue stained for FOXA2 using ab228350 at 1/250 dilution in immunohistochemical analysis (green)
The nuclear counter stain is fluoroshield with DAPI (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228350 has not yet been referenced specifically in any publications.