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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box Other FOXes
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RecombinantRabMAb

Recombinant Anti-FOXB2 antibody [EPR11476] (ab170897)

  • Datasheet
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Immunoprecipitation - Anti-FOXB2 antibody [EPR11476] (ab170897)
  • Western blot - Anti-FOXB2 antibody [EPR11476] (ab170897)
  • Anti-FOXB2 antibody [EPR11476] (ab170897)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11476] to FOXB2
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-FOXB2 antibody [EPR11476]
    See all FOXB2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11476] to FOXB2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FOXB2 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q5VYV0

  • Positive control

    • HeLa and LoVo cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11476
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Other FOXes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Alternative Versions

    • Anti-FOXB2 antibody [EPR11476] - BSA and Azide free (ab249575)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

Our Abpromise guarantee covers the use of ab170897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Transcription factor.
    • Sequence similarities

      Contains 1 fork-head DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q5VYV0 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 442425 Human
      • Entrez Gene: 14240 Mouse
      • Entrez Gene: 691398 Rat
      • SwissProt: Q5VYV0 Human
      • SwissProt: Q64733 Mouse
      • Unigene: 553843 Human
      • Unigene: 7971 Mouse
      • Alternative names

        • bA159H20.4 antibody
        • Forkhead box B2 antibody
        • Forkhead box protein B2 antibody
        • FOXB 2 antibody
        • Foxb2 antibody
        • FOXB2_HUMAN antibody
        see all

      Images

      • Immunoprecipitation - Anti-FOXB2 antibody [EPR11476] (ab170897)
        Immunoprecipitation - Anti-FOXB2 antibody [EPR11476] (ab170897)

        Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170897 and HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

        Lane 1 Primary: ab170897, 1/1000 dilution
        Sample: Immunoprecipitation pellet from HeLa cell lysate
        Secondary: HRP-conjugated anti-rabbit IgG

         

         

        Lane 2 Primary: ab170897, 1/1000 dilution
        Sample: 1X PBS (negative control)
        Secondary: HRP-conjugated anti-rabbit IgG

         

         

      • Western blot - Anti-FOXB2 antibody [EPR11476] (ab170897)
        Western blot - Anti-FOXB2 antibody [EPR11476] (ab170897)
        All lanes : Anti-FOXB2 antibody [EPR11476] (ab170897) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : LoVo cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 45 kDa

      • Anti-FOXB2 antibody [EPR11476] (ab170897)
        Anti-FOXB2 antibody [EPR11476] (ab170897)

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab170897? Please let us know so that we can cite the reference in this datasheet.

      ab170897 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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