Recombinant Anti-FOXB2 antibody [EPR11476] (ab170897)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FOXB2 antibody [EPR11476]
See all FOXB2 primary antibodies -
Description
Rabbit monoclonal [EPR11476] to FOXB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FOXB2 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q5VYV0 -
Positive control
- HeLa and LoVo cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11476 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 45 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Transcription factor. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 442425 Human
- Entrez Gene: 14240 Mouse
- Entrez Gene: 691398 Rat
- SwissProt: Q5VYV0 Human
- SwissProt: Q64733 Mouse
- Unigene: 553843 Human
- Unigene: 7971 Mouse
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Alternative names
- bA159H20.4 antibody
- Forkhead box B2 antibody
- Forkhead box protein B2 antibody
see all
Images
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![Immunoprecipitation - Anti-FOXB2 antibody [EPR11476] (ab170897)](https://www.abcam.com/ps/products/170/ab170897/Images/ab170897-170832-anti-foxb2-antibody-epr11476-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FOXB2 antibody [EPR11476] (ab170897)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170897 and HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
Lane 1 Primary: ab170897, 1/1000 dilution
Sample: Immunoprecipitation pellet from HeLa cell lysate
Secondary: HRP-conjugated anti-rabbit IgGLane 2 Primary: ab170897, 1/1000 dilution
Sample: 1X PBS (negative control)
Secondary: HRP-conjugated anti-rabbit IgG -
![Western blot - Anti-FOXB2 antibody [EPR11476] (ab170897)](https://www.abcam.com/ps/products/170/ab170897/Images/ab170897-170831-anti-foxb2-antibody-epr11476-western-blot.jpg)
Western blot - Anti-FOXB2 antibody [EPR11476] (ab170897)All lanes : Anti-FOXB2 antibody [EPR11476] (ab170897) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LoVo cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Datasheets and documents
References
ab170897 has not yet been referenced specifically in any publications.
