Overview

  • Product name
    Anti-FOXB2 antibody [EPR11476]
  • Description
    Rabbit monoclonal [EPR11476] to FOXB2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FOXB2 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q5VYV0

  • Positive control
    • HeLa and LoVo cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    Images

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170897 and HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

      Lane 1 Primary: ab170897, 1/1000 dilution
      Sample: Immunoprecipitation pellet from HeLa cell lysate
      Secondary: HRP-conjugated anti-rabbit IgG

       

       

      Lane 2 Primary: ab170897, 1/1000 dilution
      Sample: 1X PBS (negative control)
      Secondary: HRP-conjugated anti-rabbit IgG

       

       

    • All lanes : Anti-FOXB2 antibody [EPR11476] (ab170897) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : LoVo cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 45 kDa

    References

    ab170897 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170897.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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