Recombinant Anti-FOXB2 antibody [EPR11476] (ab170897)
![Immunoprecipitation - Anti-FOXB2 antibody [EPR11476] (ab170897)](https://www.abcam.com/ps/products/170/ab170897/Images/ab170897-170832-anti-foxb2-antibody-epr11476-immunoprecipitation.jpg "Immunoprecipitation - Anti-FOXB2 antibody [EPR11476] (ab170897)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11476] to FOXB2
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-FOXB2 antibody [EPR11476]
See all FOXB2 primary antibodies -
Description
Rabbit monoclonal [EPR11476] to FOXB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FOXB2 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q5VYV0 -
Positive control
- HeLa and LoVo cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11476 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 45 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Transcription factor. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 442425 Human
- Entrez Gene: 14240 Mouse
- Entrez Gene: 691398 Rat
- SwissProt: Q5VYV0 Human
- SwissProt: Q64733 Mouse
- Unigene: 553843 Human
- Unigene: 7971 Mouse
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Alternative names
- bA159H20.4 antibody
- Forkhead box B2 antibody
- Forkhead box protein B2 antibody
see all
Images
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Immunoprecipitation - Anti-FOXB2 antibody [EPR11476] (ab170897)
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170897 and HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
Lane 1 Primary: ab170897, 1/1000 dilution
Sample: Immunoprecipitation pellet from HeLa cell lysate
Secondary: HRP-conjugated anti-rabbit IgGLane 2 Primary: ab170897, 1/1000 dilution
Sample: 1X PBS (negative control)
Secondary: HRP-conjugated anti-rabbit IgG -
![Western blot - Anti-FOXB2 antibody [EPR11476] (ab170897)](https://www.abcam.com/ps/products/170/ab170897/Images/ab170897-170831-anti-foxb2-antibody-epr11476-western-blot.jpg)
Western blot - Anti-FOXB2 antibody [EPR11476] (ab170897)All lanes : Anti-FOXB2 antibody [EPR11476] (ab170897) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LoVo cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa -
![Anti-FOXB2 antibody [EPR11476] (ab170897)](https://www.abcam.com/ps/products/170/ab170897/Images/ab170897-3-benefits-of-recombinant-antibodies.png)
Anti-FOXB2 antibody [EPR11476] (ab170897)
Protocols
Datasheets and documents
References (0)
ab170897 has not yet been referenced specifically in any publications.
