Overview

  • Product name
    Anti-FOXC1 antibody - ChIP Grade
    See all FOXC1 primary antibodies
  • Description
    Goat polyclonal to FOXC1 - ChIP Grade
  • Host species
    Goat
  • Tested applications
    Suitable for: ChIP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Zebrafish
    Predicted to work with: Xenopus laevis, Catfish
  • Immunogen

    Synthetic peptide:

    RTSGAFVYDCSKF

    , corresponding to amino acids 541-553 of Human FOXC 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab5079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution. PubMed: 17000708
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 57 kDa.Can be blocked with Human FOXC1 peptide (ab23069).
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees.
  • Tissue specificity
    Expressed in all tissues and cell lines examined.
  • Involvement in disease
    Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3) [MIM:602482]; also known as Axenfeld-Rieger syndrome (ARS) or Axenfeld syndrome or Axenfeld anomaly. It is characterized by posterior corneal embryotoxon, prominent Schwalbe line and iris adhesion to the Schwalbe line. Other features may be hypertelorism (wide spacing of the eyes), hypoplasia of the malar bones, congenital absence of some teeth and mental retardation. When associated with tooth anomalies, the disorder is known as Rieger syndrome. Glaucoma is a progressive blinding condition that occurs in approximately half of patients with Axenfeld-Rieger malformations.
    Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA) [MIM:601631]. IGDA is an autosomal dominant phenotype characterized by iris hypoplasia, goniodysgenesis, and juvenile glaucoma.
    Defects in FOXC1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARA antibody
    • FKH L7 antibody
    • FKHL 7 antibody
    • FKHL7 antibody
    • Forkhead (Drosophila) like 7 antibody
    • Forkhead box C1 antibody
    • Forkhead box protein C1 antibody
    • Forkhead drosophila homolog like 7 antibody
    • Forkhead like 7 antibody
    • Forkhead related activator 3 antibody
    • Forkhead related protein FKHL7 antibody
    • Forkhead related transcription factor 3 antibody
    • Forkhead-related protein FKHL7 antibody
    • Forkhead-related transcription factor 3 antibody
    • FOX C1 antibody
    • FOXC 1 antibody
    • Foxc1 antibody
    • FOXC1_HUMAN antibody
    • FREAC 3 antibody
    • FREAC-3 antibody
    • FREAC3 antibody
    • IGDA antibody
    • IHG 1 antibody
    • IHG1 antibody
    • IRID 1 antibody
    • IRID1 antibody
    • Iridogoniodysgenesis type 1 antibody
    • Myeloid factor delta antibody
    see all

Images

  • ab5079 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. 

  • Lane 1 : Anti-FOXC1 antibody - ChIP Grade (ab5079) at 0.5 µg/ml
    Lane 2 : Anti-FOXC1 antibody - ChIP Grade (ab5079) at 0.5 mg/ml
    Lane 3 : anti- DYKDDDDK Tag at 1/3000 dilution

    Lanes 1 & 3 : HEK293 lysate (in RIPA buffer)
    Lane 2 : Mock-transfected HEK293 lysate (in RIPA buffer)

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 57 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Primary incubations were for 1 hour.

    Detected by chemiluminescence.

  • ab5079 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • Mouse tissue sections (periocular mesenchyme, cornea) were incubated with ab5079, anti-FOXC1 antibody. Paraformaldehyde was used for fixation, and a heat mediated antigen retrieval step was used. The antibody was incubated for 8 hours at a dilution of 1/200.
  • ab5079 at 3ug/ml staining FOXC1 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, staining FOXC1 with ab5079 at 4 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).

References

This product has been referenced in:
  • Wu J  et al. CircIRAK3 sponges miR-3607 to facilitate breast cancer metastasis. Cancer Lett 430:179-192 (2018). WB . Read more (PubMed: 29803789) »
  • Pan H  et al. Forkhead box C1 boosts triple-negative breast cancer metastasis through activating the transcription of chemokine receptor-4. Cancer Sci 109:3794-3804 (2018). Read more (PubMed: 30290049) »
See all 22 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (MV411 transfected with FOXC1 plasmid)
Total protein in input
20 cells
Immuno-precipitation step
Protein G
Specification
MV411 transfected with FOXC1 plasmid

Fabrizio Simeoni

Verified customer

Submitted Mar 28 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (T47D)
Loading amount
100 µg
Specification
T47D
Treatment
10 nM si RNA 48 h
Blocking step
Serum, BSA, milk sequential incubations as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%

Dr. F Stanley

Verified customer

Submitted Mar 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (periocular mesenchyme, cornea)
Specification
periocular mesenchyme, cornea
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

Ms. Amanda Evans-Zacharias

Verified customer

Submitted Feb 08 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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