RecombinantRabMAb

Recombinant Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
  • Immunoprecipitation - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
  • Flow Cytometry - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
  • Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20678] to FOXC1 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FOXC1 antibody [EPR20678] - BSA and Azide free
    See all FOXC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20678] to FOXC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, IHC-P, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human FOXC1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q12948

  • Positive control

    • IHC-P: Human gastric cancer tissue.

  • General notes

    Ab230157 is the carrier-free version of ab223850. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab230157 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab230157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC not suitable for mouse & rat tissues.
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees.

    • Tissue specificity

      Expressed in all tissues and cell lines examined.

    • Involvement in disease

      Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3) [MIM:602482]; also known as Axenfeld-Rieger syndrome (ARS) or Axenfeld syndrome or Axenfeld anomaly. It is characterized by posterior corneal embryotoxon, prominent Schwalbe line and iris adhesion to the Schwalbe line. Other features may be hypertelorism (wide spacing of the eyes), hypoplasia of the malar bones, congenital absence of some teeth and mental retardation. When associated with tooth anomalies, the disorder is known as Rieger syndrome. Glaucoma is a progressive blinding condition that occurs in approximately half of patients with Axenfeld-Rieger malformations.
      Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA) [MIM:601631]. IGDA is an autosomal dominant phenotype characterized by iris hypoplasia, goniodysgenesis, and juvenile glaucoma.
      Defects in FOXC1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.

    • Sequence similarities

      Contains 1 fork-head DNA-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ARA antibody
      • FKH L7 antibody
      • FKHL 7 antibody
      • FKHL7 antibody
      • Forkhead (Drosophila) like 7 antibody
      • Forkhead box C1 antibody
      • Forkhead box protein C1 antibody
      • Forkhead drosophila homolog like 7 antibody
      • Forkhead like 7 antibody
      • Forkhead related activator 3 antibody
      • Forkhead related protein FKHL7 antibody
      • Forkhead related transcription factor 3 antibody
      • Forkhead-related protein FKHL7 antibody
      • Forkhead-related transcription factor 3 antibody
      • FOX C1 antibody
      • FOXC 1 antibody
      • Foxc1 antibody
      • FOXC1_HUMAN antibody
      • FREAC 3 antibody
      • FREAC-3 antibody
      • FREAC3 antibody
      • IGDA antibody
      • IHG 1 antibody
      • IHG1 antibody
      • IRID 1 antibody
      • IRID1 antibody
      • Iridogoniodysgenesis type 1 antibody
      • Myeloid factor delta antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

      Immunohistochemical analysis of paraffin-embedded human basal-like breast cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human basal-like breast cancer (PMID:27708239; PMID: 20406990). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
      Immunoprecipitation - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

      FOXC1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab223850 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223850 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: HeLa whole cell lysate 10 µg (Input). 

      Lane 2: ab223850 IP in HeLa whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223850 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      The molecular mass is consistent with what has been described in the literature (PMID: 22493429).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

    • Flow Cytometry - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
      Flow Cytometry - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line labeling FOXC1 with ab223850 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

      Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human gastric cancer is observed(PMID:24329718). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

      Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human pancreatic cancer (PMID:23242609). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
      Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab230157? Please let us know so that we can cite the reference in this datasheet.

    ab230157 has not yet been referenced specifically in any publications.

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