Recombinant
RabMAb

Recombinant Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

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Overview

  • Product name

    Anti-FOXC1 antibody [EPR20678] - BSA and Azide free
    See all FOXC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20678] to FOXC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human FOXC1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q12948

  • Positive control

    • IHC-P: Human gastric cancer tissue.

  • General notes

    Ab230157 is the carrier-free version of ab223850. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab230157 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab230157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC not suitable for mouse & rat tissues.
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees.

  • Tissue specificity

    Expressed in all tissues and cell lines examined.

  • Involvement in disease

    Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3) [MIM:602482]; also known as Axenfeld-Rieger syndrome (ARS) or Axenfeld syndrome or Axenfeld anomaly. It is characterized by posterior corneal embryotoxon, prominent Schwalbe line and iris adhesion to the Schwalbe line. Other features may be hypertelorism (wide spacing of the eyes), hypoplasia of the malar bones, congenital absence of some teeth and mental retardation. When associated with tooth anomalies, the disorder is known as Rieger syndrome. Glaucoma is a progressive blinding condition that occurs in approximately half of patients with Axenfeld-Rieger malformations.
    Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA) [MIM:601631]. IGDA is an autosomal dominant phenotype characterized by iris hypoplasia, goniodysgenesis, and juvenile glaucoma.
    Defects in FOXC1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.

  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ARA antibody
    • FKH L7 antibody
    • FKHL 7 antibody
    • FKHL7 antibody
    • Forkhead (Drosophila) like 7 antibody
    • Forkhead box C1 antibody
    • Forkhead box protein C1 antibody
    • Forkhead drosophila homolog like 7 antibody
    • Forkhead like 7 antibody
    • Forkhead related activator 3 antibody
    • Forkhead related protein FKHL7 antibody
    • Forkhead related transcription factor 3 antibody
    • Forkhead-related protein FKHL7 antibody
    • Forkhead-related transcription factor 3 antibody
    • FOX C1 antibody
    • FOXC 1 antibody
    • Foxc1 antibody
    • FOXC1_HUMAN antibody
    • FREAC 3 antibody
    • FREAC-3 antibody
    • FREAC3 antibody
    • IGDA antibody
    • IHG 1 antibody
    • IHG1 antibody
    • IRID 1 antibody
    • IRID1 antibody
    • Iridogoniodysgenesis type 1 antibody
    • Myeloid factor delta antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

    Immunohistochemical analysis of paraffin-embedded human basal-like breast cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human basal-like breast cancer (PMID:27708239; PMID: 20406990). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
    Immunoprecipitation - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

    FOXC1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab223850 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223850 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). 

    Lane 2: ab223850 IP in HeLa whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223850 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The molecular mass is consistent with what has been described in the literature (PMID: 22493429).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

  • Flow Cytometry - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
    Flow Cytometry - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line labeling FOXC1 with ab223850 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling FOXC1 with ab223850 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HEK-293T cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling FOXC1 with ab223850 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HEK-293T cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human gastric cancer is observed(PMID:24329718). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] - BSA and Azide free (ab230157)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human pancreatic cancer (PMID:23242609). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223850).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab230157 has not yet been referenced specifically in any publications.

Publishing research using ab230157? Please let us know so that we can cite the reference in this datasheet.

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