Product nameAnti-FOXC2 antibody
See all FOXC2 primary antibodies
DescriptionRabbit polyclonal to FOXC2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human FOXC2 aa 451-501. The exact sequence is proprietary. NP_005242.1
Database link: Q99958
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245510 was affinity purified using an epitope specific to FOXC2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000.|
FunctionTranscriptional activator. Might be involved in the formation of special mesenchymal tissues.
Involvement in diseaseDefects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2) [MIM:153200]; also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment.
Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN) [MIM:153300]. LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities.
Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD) [MIM:153400]. LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).
Sequence similaritiesContains 1 fork-head DNA-binding domain.
- Information by UniProt
- Drosphilia Forkhead Homolog Like 14 antibody
- FKHL 14 antibody
- FKHL14 antibody
FOXC2 was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20% of IP loaded) using ab245510 at 10 µg/mg lysate. Western blot was perfromed on the immunoprecipitate using ab245510 at 1 µg/ml.
Lane 1: ab245510 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes : Anti-FOXC2 antibody (ab245510) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Exposure time: 3 minutes
ab245510 has not yet been referenced specifically in any publications.