Anti-FOXC2 antibody (ab5060)
Key features and details
- Goat polyclonal to FOXC2
- Suitable for: WB, ChIP
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-FOXC2 antibody
See all FOXC2 primary antibodies -
Description
Goat polyclonal to FOXC2 -
Host species
Goat -
Tested applications
Suitable for: WB, ChIPmore details
Unsuitable for: IHC-Fr -
Species reactivity
Reacts with: Mouse, Human, African green monkey
Predicted to work with: Rat, Cow, Dog -
Immunogen
Synthetic peptide:
RHAAPYSYDCTKY
, corresponding to C terminal amino acids 489-501 of Human FOXC2. -
Positive control
- WB: HEK-293 nuclear lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 2 µg/ml. Detects a band of approximately 60-70 kDa (predicted molecular weight: 53 kDa).
A 1 hour primary incubation is recommended for this product. |
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ChIP |
Use at an assay dependent concentration. PubMed: 18579532
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Notes |
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WB
Use a concentration of 0.1 - 2 µg/ml. Detects a band of approximately 60-70 kDa (predicted molecular weight: 53 kDa). A 1 hour primary incubation is recommended for this product. |
ChIP
Use at an assay dependent concentration. PubMed: 18579532 |
Target
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Function
Transcriptional activator. Might be involved in the formation of special mesenchymal tissues. -
Involvement in disease
Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2) [MIM:153200]; also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment.
Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN) [MIM:153300]. LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities.
Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD) [MIM:153400]. LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices). -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2303 Human
- Entrez Gene: 14234 Mouse
- Entrez Gene: 171356 Rat
- Omim: 602402 Human
- SwissProt: Q99958 Human
- SwissProt: Q61850 Mouse
- SwissProt: Q63246 Rat
- Unigene: 436448 Human
see all -
Alternative names
- Drosphilia Forkhead Homolog Like 14 antibody
- FKHL 14 antibody
- FKHL14 antibody
see all
Images
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Lane 1 : Anti-FOXC2 antibody (ab5060) at 2 µg/ml
Lane 2 : Anti-FOXC2 antibody (ab5060) at 1 µg/ml
Lane 1 : nuclear HEK293 lysate in RIPA buffer
Lane 2 : human pancreas whole cell lysate (negative control) in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 60-70 kDa why is the actual band size different from the predicted?Primary incubation: 1 hour at room temperature.
Negative Control: Human Pancreas lysate.
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FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector.
Datasheets and documents
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Datasheet download
References (27)
ab5060 has been referenced in 27 publications.
- Lin F et al. Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer. J Exp Clin Cancer Res 41:129 (2022). PubMed: 35392966
- Liu H et al. The FENDRR/FOXC2 Axis Contributes to Multidrug Resistance in Gastric Cancer and Correlates With Poor Prognosis. Front Oncol 11:634579 (2021). PubMed: 33869020
- Norden PR et al. Shear stimulation of FOXC1 and FOXC2 differentially regulates cytoskeletal activity during lymphatic valve maturation. Elife 9:N/A (2020). PubMed: 32510325
- Tavian D et al. FOXC2 Disease Mutations Identified in Lymphedema Distichiasis Patients Impair Transcriptional Activity and Cell Proliferation. Int J Mol Sci 21:N/A (2020). PubMed: 32698337
- Zhang Y et al. Activation of mitogen-activated protein kinases in satellite glial cells of the trigeminal ganglion contributes to substance P-mediated inflammatory pain. Int J Oral Sci 11:24 (2019). PubMed: 31501412