Key features and details
- Goat polyclonal to FOXC2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FOXC2 antibody
See all FOXC2 primary antibodies
DescriptionGoat polyclonal to FOXC2
SpecificityThe antibody labels FOXC2 in mouse lymphatic primordia, jugular lymph sacs, lymphatic collectors and capillaries. In western blotting the antibody has been tested on FOXC2 overexpressing cell lines but not on endogenous protein.
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-Fr
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog
- WB: FOXC2 overexpressing cell lines; HEK293 nuclear lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ChIP Related Products
Our Abpromise guarantee covers the use of ab5060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 2 µg/ml. Detects a band of approximately 60-70 kDa (predicted molecular weight: 53 kDa).
A 1 hour primary incubation is recommended for this product.
FunctionTranscriptional activator. Might be involved in the formation of special mesenchymal tissues.
Involvement in diseaseDefects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2) [MIM:153200]; also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment.
Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN) [MIM:153300]. LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities.
Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD) [MIM:153400]. LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).
Sequence similaritiesContains 1 fork-head DNA-binding domain.
- Information by UniProt
- Drosphilia Forkhead Homolog Like 14 antibody
- FKHL 14 antibody
- FKHL14 antibody
Lane 1 : Anti-FOXC2 antibody (ab5060) at 2 µg/ml
Lane 2 : Anti-FOXC2 antibody (ab5060) at 1 µg/ml
Lane 1 : nuclear HEK293 lysate in RIPA buffer
Lane 2 : human pancreas whole cell lysate (negative control) in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 60-70 kDa why is the actual band size different from the predicted?
Primary incubation: 1 hour at room temperature.
ab5060 has been referenced in 22 publications.
- Zhou LJ et al. Microglia Are Indispensable for Synaptic Plasticity in the Spinal Dorsal Horn and Chronic Pain. Cell Rep 27:3844-3859.e6 (2019). PubMed: 31242418
- Trost A et al. Lymphatic markers in the human optic nerve. Exp Eye Res 173:113-120 (2018). IHC ; Human . PubMed: 29746818
- Munger SJ et al. Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice. Dev Biol 412:173-90 (2016). PubMed: 26953188
- Fatima A et al. Foxc1 and Foxc2 deletion causes abnormal lymphangiogenesis and correlates with ERK hyperactivation. J Clin Invest 126:2437-51 (2016). ChIP ; Mouse . PubMed: 27214551
- Kanady JD et al. Combining Foxc2 and Connexin37 deletions in mice leads to severe defects in lymphatic vascular growth and remodeling. Dev Biol 405:33-46 (2015). PubMed: 26079578