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Our Abpromise guarantee covers the use of ab5060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).Can be blocked with Human FOXC2 peptide (ab23061).|
|ChIP||Use at an assay dependent concentration. PubMed: 18579532|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab5060 (2µg/ml) staining of paraffin embedded Human Breast cancer tissue shows nuclear staning with some weak cytoplasm staining. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
The primary antibody incubated for 1 hour.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"