Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A region within synthetic peptide: TGAGTGGGAK NPLVKPPYSY IALITMAILQ SPKKRLTLSE ICEFISSRFP, corresponding to N terminal amino acids 116-165 of Mouse FOXD1

  • Positive control
    • SP2/0 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Transcription factor required for formation of positional identity in the developing retina, regionalization of the optic chiasm and morphogenesis of the kidney. Can neuralize ectodermal cells directly.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain Factor 2 antibody
    • FKH L8 antibody
    • FKHL 8 antibody
    • FKHL8 antibody
    • Forkhead (Drosophila) like 8 antibody
    • Forkhead box D1 antibody
    • Forkhead box protein D1 antibody
    • Forkhead drosophila homolog like 8 antibody
    • Forkhead like 8 antibody
    • Forkhead related activator 4 antibody
    • Forkhead related protein FKHL8 antibody
    • Forkhead related transcription factor 4 antibody
    • Forkhead-related protein FKHL8 antibody
    • Forkhead-related transcription factor 4 antibody
    • FOX D1 antibody
    • FOXD 1 antibody
    • foxd1 antibody
    • FOXD1_HUMAN antibody
    • FREAC 4 antibody
    • FREAC-4 antibody
    • FREAC4 antibody
    see all

Images

  • Anti-FOXD1 antibody (ab49156) at 1.25 µg/ml + SP2/0 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50,000 - 1/100,000

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (retinal ganglion cells)
Specification
retinal ganglion cells
Permeabilization
Yes - 0.1% Triton X
Fixative
Paraformaldehyde

Dr. Ryan Macdonald

Verified customer

Submitted Feb 24 2014

Answer

Thank you for submitting an Abreview of ab49156. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untestedapplication, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

-try to include an enzymatic antigen retrieval step

If you decide to follow these suggestions, please let me know if they are helpful. Should this not work, then that could indicate that the antibody might not be suitable for IHC-Fr.

I wish you good luck with your research.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse embryonic kidney)
Specification
Mouse embryonic kidney
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 06 2012

Question
Answer


I am very pleased to hear you would like to accept our offer and test ab49156 inIHC-P. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (LNCaP-1F5 Cell line)
Loading amount
30 µg
Specification
LNCaP-1F5 Cell line
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Biswajyoti Sahu

Verified customer

Submitted Jul 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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