Key features and details
- Rabbit polyclonal to FOXD3
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FOXD3 antibody
See all FOXD3 primary antibodies
DescriptionRabbit polyclonal to FOXD3
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
A synthesized peptide derived from an internal region of human FOXD3.
- Human heart tissue and extracts from HUVEC and 293 cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
FunctionBinds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
Tissue specificityExpressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.
Involvement in diseaseDefects in FOXD3 are associated with susceptibility to autoimmune disease type 1 (AIS1) [MIM:607836]. AIS1 is a disorder characterized by the association of vitiligo with autoimmune thyroiditis (Hashimoto thyroiditis).
Sequence similaritiesContains 1 fork-head DNA-binding domain.
- Information by UniProt
- AIS1 antibody
- forkhead box D3 antibody
- Forkhead box protein D3 antibody
All lanes : Anti-FOXD3 antibody (ab64807) at 1/500 dilution
Lane 1 : Extracts of HUVEC cells
Lane 2 : Extracts of 293 cells
Lane 3 : Extracts of 293 cells, plus immunising peptide at 5-10µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64807 at a 1:50 dilution.
Left image untreated.Right image treated with immunising peptide.
ab64807 has been referenced in 1 publication.
- Douglas GC et al. Trophoblast stem cells: models for investigating trophectoderm differentiation and placental development. Endocr Rev 30:228-40 (2009). PubMed: 19299251