Overview

  • Product name

  • Description

    Rabbit polyclonal to FOXD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthesized peptide derived from an internal region of human FOXD3.

  • Positive control

    • Human heart tissue and extracts from HUVEC and 293 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab64807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ELISA 1/10000.

Target

  • Function

    Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
  • Tissue specificity

    Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.
  • Involvement in disease

    Defects in FOXD3 are associated with susceptibility to autoimmune disease type 1 (AIS1) [MIM:607836]. AIS1 is a disorder characterized by the association of vitiligo with autoimmune thyroiditis (Hashimoto thyroiditis).
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AIS1 antibody
    • forkhead box D3 antibody
    • Forkhead box protein D3 antibody
    • foxd3 antibody
    • FOXD3_HUMAN antibody
    • Genesis antibody
    • HFH2 antibody
    • HNF3/FH transcription factor genesis antibody
    • VAMAS2 antibody
    see all

Images

  • All lanes : Anti-FOXD3 antibody (ab64807) at 1/500 dilution

    Lane 1 : Extracts of HUVEC cells
    Lane 2 : Extracts of 293 cells
    Lane 3 : Extracts of 293 cells, plus immunising peptide at 5-10µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64807 at a 1:50 dilution.
    Left image untreated.
    Right image treated with immunising peptide.

References

This product has been referenced in:

  • Douglas GC  et al. Trophoblast stem cells: models for investigating trophectoderm differentiation and placental development. Endocr Rev 30:228-40 (2009). Read more (PubMed: 19299251) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry.

I contacted the producing labs of these two antibodies. Although the immunogen sequences are confidential, they did an alignment with the sequence you provided.

Unfortunately, both immunogen sequences did not show any significantly homology with the sequence you provided and I therefore do not recommend these antibodies for your purposes.

I am sorry I did not have a positive answer to your question on this occasion and wish you the best of luck with your research.

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