Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide within FOXF1 aa 292-341 (C terminal). The exact sequence is proprietary.
    Sequence:

    PCNPAANPLSGSLSTHSLEQPYLHQNSHNAPAELQGIPRYHSQSPSMCDR


    Database link: Q12946

  • Positive control
    • Fetal placental lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab23194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21341990
IHC-P Use at an assay dependent concentration. PubMed: 20722018
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Probable transcription activator for a number of lung-specific genes.
  • Tissue specificity
    Lung and placenta.
  • Involvement in disease
    Defects in FOXF1 are the cause of alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) [MIM:265380]. ACDMPV is a rare malformation due to abnormal development of the capillary vascular system in the lungs. Histologically, it is characterized by failure of formation and ingrowth of alveolar capillaries, medial muscular thickening of small pulmonary arterioles with muscularization of the intraacinar arterioles, thickened alveolar walls, and anomalously situated pulmonary veins running alongside pulmonary arterioles and sharing the same adventitial sheath. Less common features include a reduced number of alveoli and a patchy distribution of the histopathologic changes. Affected infants present with respiratory distress and the disease is fatal within the newborn period. Additional features include multiple congenital anomalies affecting the cardiovascular, gastrointestinal, genitourinary, and musculoskeletal systems, as well as disruption of the normal right-left asymmetry of intrathoracic or intraabdominal organs. ACDMPV is a rare cause of persistent pulmonary hypertension of the newborn, an abnormal physiologic state caused by failure of transition of the pulmonary circulation from the high pulmonary vascular resistance of the fetus to the low pulmonary vascular resistance of the newborn.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Domain
    Activation domains C-terminal of (and distinct from) the forkhead domains are necessary for transcriptional activation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI450827 antibody
    • FKH L5 antibody
    • FKHL 5 antibody
    • FKHL5 antibody
    • Forkhead (Drosophila) like 5 antibody
    • Forkhead box F1 antibody
    • Forkhead box protein F1 antibody
    • Forkhead drosophila homolog like 5 antibody
    • Forkhead like 5 antibody
    • Forkhead related activator 1 antibody
    • Forkhead related protein FKHL5 antibody
    • Forkhead related transcription factor 1 antibody
    • Forkhead-related activator 1 antibody
    • Forkhead-related protein FKHL5 antibody
    • Forkhead-related transcription factor 1 antibody
    • FOX F1 antibody
    • FOXF 1 antibody
    • foxf1 antibody
    • FOXF1_HUMAN antibody
    • FREAC 1 antibody
    • FREAC-1 antibody
    • FREAC1 antibody
    • HFH 8 antibody
    • MGC105125 antibody
    see all

Images

References

This product has been referenced in:
  • Wei HJ  et al. FOXF1 mediates mesenchymal stem cell fusion-induced reprogramming of lung cancer cells. Oncotarget 5:9514-29 (2014). WB ; Human . Read more (PubMed: 25237908) »
  • Bozyk PD  et al. Mesenchymal stromal cells from neonatal tracheal aspirates demonstrate a pattern of lung-specific gene expression. Stem Cells Dev 20:1995-2007 (2011). ICC/IF ; Human . Read more (PubMed: 21341990) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

DISCOUNT CODE: xxxx
Expiration date: 27th of February 2013

I am very pleased to hear you would like to accept our offer and test ab23194 with your mouse samples.This code will give you the value of ab23194 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site:

www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

www.abcam.com/abtrial

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Answer

Merci de nous avoir contactés.

Je suis trèsdésolée que l'utilisation de la BSA n'a pas pu améliorer les résultats. J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN xx

Voici les manières dont vous pouvez utiliser cette référence CN:
(1) la faire valoir contre la facture originale si celle-ci n'as pas encore été payée
(2) la faire valoir contre une prochaine facture
(3) obtenir un remboursement de la somme entière si vous n'avez pas de factures actuellement en cours avec Abcam

Si vous souhaitez recevoir un remboursement au lieu d'un avoir, veuillez demander à votre service de gestion de prendre contact avec notre département comptabilité afin que nous puissions recueillir les informations nécessaires pour cette restitution. Notre service comptabilité peut être contacté par courriel à l'adresse creditcontrol@abcam.com ou par téléphone en utilisant le lien « Contactez-nous » dans le coin en haut à droite de notre site internet. Veuillez communiquer votre numéro d'avoir lorsque vous contactez notre service comptabilité.

Vous recevrez également la référence complète de la note de crédit par courriel ou par voie postale; cette référence commençant par les lettres CGB.

J'espère que cette expérience ne vous empêchera pas de commander à nouveau l'un de nos produits.

L'équipe technique reste à votre disposition pour plus de conseils.

Bonne continuation dans votre recherche.

Read More

Answer

Thank you for your phone call.

The free of charge replacement information for ab52356 can be found below. I am also sending youthe testing discount code for trying ab23194 in mouse - at the bottom of the email.


I am sorry to hear that our product ab52356 did not perform as before and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx.

To check the status of the order please contact our Customer Service team and reference this number.

Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

====================================================

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab23194 in mouse. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for mouseand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I wish you the best of luck with your research.

Read More

Answer

Merci de nous avoir contacté. Je suis désolée d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab23194.

Notre politique est d'offrir tout d'abord la meilleure assistance technique. Si nous concluons que le produit que vous avez reçu ne fonctionne pas comme mentionné sur sa fiche technique et que ce produit a été commandé sous 180 jours, nous nous ferons un plaisir de vous offrir un remplacement gratuit ou un avoir en compensation.

Comme discuté au téléphone, je suggèred'utiliser aussi un bloquage avec de la BSA. Ceci peux singificativement améliorer le résultat

J'espère que ceci va resoudre le problème.Veuillez s'il vous plaît nous tenir au courant.

En attendant de vos nouvelles, je vous souhaite une très agréable fin de semaine.

Read More

Answer

Thank you for contacting us and your interest in our products.

I can confirm that the antibodies ab5081 and ab23194 have been raised against different immunogens. The antibody ab5081 was raised against a synthetic peptide taken from the residues 2-14 of FOX F1 whilst the immunogen used to raise ab23194 was raised against a peptide corresponding to the amino acids 273-327 of FOX F1.

Although we have as yet not used ab23194 with mouse samples, as the immunogen used to raise it shares 96% homology with the mouse protein we would expect it to cross react. However as we have not tested this ourselves we would not be able to guarantee it. If you would like to test ab23194 with your mouse samples you would be eligible for the Abtrial testing scheme.

This offer means that if you purchase ab23194 as normal, test the antibody with your mouse samples and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab23194 off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab23194.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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