Recombinant Anti-FOXF1 antibody [EPR7971] (ab168383)
![Western blot - Anti-FOXF1 antibody [EPR7971] (ab168383)](https://www.abcam.com/ps/products/168/ab168383/Images/ab168383-351368-anti-foxf1-antibody-epr7971-westernblot-human.jpg "Western blot - Anti-FOXF1 antibody [EPR7971] (ab168383)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7971] to FOXF1
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-FOXF1 antibody [EPR7971]
See all FOXF1 primary antibodies -
Description
Rabbit monoclonal [EPR7971] to FOXF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human FOXF1. The exact sequence is proprietary.
Database link: Q12946 -
Positive control
- Flow Cyt: HeLa cells; ICC/IF: HeLa cells; WB: HeLa, and Human lung lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7971 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
|
|
| ICC/IF |
1/250 - 1/500.
|
|
| Flow Cyt |
1/70 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 40 kDa. |
|
ICC/IF
1/250 - 1/500. |
|
Flow Cyt
1/70 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Probable transcription activator for a number of lung-specific genes. -
Tissue specificity
Lung and placenta. -
Involvement in disease
Defects in FOXF1 are the cause of alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) [MIM:265380]. ACDMPV is a rare malformation due to abnormal development of the capillary vascular system in the lungs. Histologically, it is characterized by failure of formation and ingrowth of alveolar capillaries, medial muscular thickening of small pulmonary arterioles with muscularization of the intraacinar arterioles, thickened alveolar walls, and anomalously situated pulmonary veins running alongside pulmonary arterioles and sharing the same adventitial sheath. Less common features include a reduced number of alveoli and a patchy distribution of the histopathologic changes. Affected infants present with respiratory distress and the disease is fatal within the newborn period. Additional features include multiple congenital anomalies affecting the cardiovascular, gastrointestinal, genitourinary, and musculoskeletal systems, as well as disruption of the normal right-left asymmetry of intrathoracic or intraabdominal organs. ACDMPV is a rare cause of persistent pulmonary hypertension of the newborn, an abnormal physiologic state caused by failure of transition of the pulmonary circulation from the high pulmonary vascular resistance of the fetus to the low pulmonary vascular resistance of the newborn. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Domain
Activation domains C-terminal of (and distinct from) the forkhead domains are necessary for transcriptional activation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2294 Human
- Omim: 601089 Human
- SwissProt: Q12946 Human
- Unigene: 155591 Human
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Alternative names
- AI450827 antibody
- FKH L5 antibody
- FKHL 5 antibody
see all
Images
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Western blot - Anti-FOXF1 antibody [EPR7971] (ab168383)All lanes : Anti-FOXF1 antibody [EPR7971] (ab168383) at 1/5000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human lung lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDaThis antibody detects high background
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![Immunocytochemistry/ Immunofluorescence - Anti-FOXF1 antibody [EPR7971] (ab168383)](https://www.abcam.com/ps/products/168/ab168383/Images/ab168383-4-anti-foxf1-antibody-epr7971-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FOXF1 antibody [EPR7971] (ab168383)Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FOXF1 with purified ab168383 at 1:250 dilution (2.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry - Anti-FOXF1 antibody [EPR7971] (ab168383)](https://www.abcam.com/ps/products/168/ab168383/Images/ab168383-5-anti-foxf1-antibody-epr7971-flowcytometry-hela-human.jpg)
Flow Cytometry - Anti-FOXF1 antibody [EPR7971] (ab168383)Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FOXF1 with purified ab168383 at 1/70 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). -
![Anti-FOXF1 antibody [EPR7971] (ab168383)](https://www.abcam.com/ps/products/168/ab168383/Images/ab168383-7-benefits-of-recombinant-antibodies.png)
Anti-FOXF1 antibody [EPR7971] (ab168383)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab168383 has been referenced in 3 publications.
- Ran L et al. FOXF1 Defines the Core-Regulatory Circuitry in Gastrointestinal Stromal Tumor. Cancer Discov 8:234-251 (2018). PubMed: 29162563
- Su S et al. A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome. Cell Rep 25:3215-3228.e9 (2018). PubMed: 30540952
- Han R et al. Functional CRISPR screen identifies AP1-associated enhancer regulating FOXF1 to modulate oncogene-induced senescence. Genome Biol 19:118 (2018). PubMed: 30119690