Overview

  • Product name

  • Description

    Rabbit polyclonal to FOXF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFr, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human FOXF2 (C terminal).
    Database link: Q12947

  • Positive control

    • Human placenta and liver tissue lysates; Human cervical cancer tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab198283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 46 kDa.

Target

  • Relevance

    FOXF2 encodes forkhead box F2, one of many human homologues of the Drosophila melanogaster transcription factor forkhead. FOXF2 is expressed in lung and placenta, and has been shown to transcriptionally activate several lung-specific genes.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • FKH L6 antibody
    • FKHL 6 antibody
    • FKHL6 antibody
    • Forkhead box F2 antibody
    • Forkhead box protein F2 antibody
    • Forkhead like 6 antibody
    • Forkhead related activator 2 antibody
    • Forkhead related protein FKHL6 antibody
    • Forkhead related transcription factor 2 antibody
    • Forkhead, Drosophila, homolog-like 6 antibody
    • FOX F2 antibody
    • FOXF 2 antibody
    • FREAC 2 antibody
    • FREAC2 antibody
    • OTTHUMP00000017863 antibody
    see all

Images

  • ab198283 staining FOXF2 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/500 in 0.1M PBST with 10% donkey serum) for 24 hours at 4°C. An Alexa Flour® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

  • All lanes : Anti-FOXF2 antibody (ab198283) at 1/666 dilution

    Lane 1 : Human placenta tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 46 kDa


    Exposure time: 5 minutes


    8% SDS PAGE

  • Immunohistochemical analysis of paraffin embedded Human cervical cancer tissue labeling FOXF2 with ab198283 at 1/30 dilution.

References

ab198283 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1 M PBS with 3% Triton X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 12 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain lysate)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Specification
Brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jan 28 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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