• Product name

  • Description

    Goat polyclonal to FOXG1
  • Host species

  • Specificity

    This antibody will recognise both human FOXG1A and FOXG1B, which are almost completely identical.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human, Xenopus laevis
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human FOXG1 aa 457-469 (C terminal).


    Database link: P55316
    (Peptide available as ab22945)

  • Positive control

    • Human Brain lysate



Our Abpromise guarantee covers the use of ab3394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500. PubMed: 17435750
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 54 kDa). The additonal unknown band at 70kDa was successfully blocked with the immunising peptide.


  • Function

    Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
  • Tissue specificity

    Expression is restricted to the neurons of the developing telencephalon.
  • Involvement in disease

    Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • BF-1 antibody
    • BF-2 antibody
    • BF1 antibody
    • BF2 antibody
    • Brain factor 1 antibody
    • Brain factor 2 antibody
    • FHKL antibody
    • FKH2 antibody
    • FKHL1 antibody
    • FKHL2 antibody
    • FKHL3 antibody
    • FKHL4 antibody
    • Forkhead box protein G1 antibody
    • Forkhead box protein G1A antibody
    • Forkhead box protein G1B antibody
    • Forkhead box protein G1C antibody
    • Forkhead like 1 antibody
    • Forkhead like 2 antibody
    • Forkhead like 3 antibody
    • Forkhead like 4 antibody
    • Forkhead-related protein FKHL1 antibody
    • Forkhead-related protein FKHL2 antibody
    • Forkhead-related protein FKHL3 antibody
    • FOXG1 antibody
    • FOXG1_HUMAN antibody
    • FOXG1A antibody
    • FOXG1B antibody
    • FOXG1C antibody
    • HBF 1 antibody
    • HBF G2 antibody
    • hBF-2 antibody
    • HBF2 antibody
    • HFK1 antibody
    • HFK2 antibody
    • HFK3 antibody
    • KHL2 antibody
    • Oncogene QIN antibody
    • QIN antibody
    see all


  • ab3394 staining (2µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab3394 (0.3 µg/ml) staining of Human Brain lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.


This product has been referenced in:

  • Li JV  et al. Transcriptional repression of AIB1 by FoxG1 leads to apoptosis in breast cancer cells. Mol Endocrinol 27:1113-27 (2013). Read more (PubMed: 23660594) »
  • Regad T  et al. The neural progenitor-specifying activity of FoxG1 is antagonistically regulated by CKI and FGF. Nat Cell Biol 9:531-40 (2007). ICC/IF ; Mouse . Read more (PubMed: 17435750) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


I have issued a free of charge replacement for ab18259, with the order number *****. The replacement is ab3394. To check the status of the order please contact our Customer Service team and reference this number. I suggested in my conversation with your colleague that an alternative to PFA fixation may give a better result this time: ice-cold acetone or methanol, or a 50:50 mixture of the two, for 10-20 minutes, followed by rinsing in buffer and blocking. No permeabilization is necessary. If you have any questions, please contact me.

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Thanks for contacting us and letting us know about the problems with ab3394. I am sending a free of charge vial of ab18259 on the order ***, which should arrive shortly. Please keep me updated on the results using this replacement, and let me know if you need anything further.

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Immunohistochemistry (Frozen sections)
Mouse Tissue sections (brain)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Oct 11 2005


Thank you very much for your enquiry. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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