Name: Anti-FOXG1 antibody [EPR18987]
Brand: RabMAb
SKU: ab196868
Rating: 4 (1 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] (ab196868)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18987] to FOXG1
Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Fr, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Alexa Fluor® 647 Carrier Free

Product name

Anti-FOXG1 antibody [EPR18987]
See all FOXG1 primary antibodies
Description

Rabbit monoclonal [EPR18987] to FOXG1
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Fr, ICC/IF, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse E12.5 brain lysate; Rat E18 brain lysate; Human fetal brain lysate; Mouse brain lysate; Rat brain lysate. IHC-P: Human glioma tissue; Mouse and rat E14 tissues. IHC-Fr: Mouse and rat embryo E14.5 tissues. ICC/IF: C6 cells. Flow Cyt (intra): C6 cells. IP: Mouse brain cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18987
Isotype

IgG
Research areas

Alternative Versions
- Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
- Alexa Fluor® 647 Anti-FOXG1 antibody [EPR18987] (ab270103)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab196868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/60.
WB		1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For mouse and rat samples we recommend a dilution of 1/2000.
IHC-Fr		1/500.
ICC/IF	(1)	1/100.
IP		1/30.

Notes
Flow Cyt (Intra) 1/60.
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For mouse and rat samples we recommend a dilution of 1/2000.
IHC-Fr 1/500.
ICC/IF 1/100.
IP 1/30.

Function

Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
Tissue specificity

Expression is restricted to the neurons of the developing telencephalon.
Involvement in disease

Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.
Sequence similarities

Contains 1 fork-head DNA-binding domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P55316 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2290 Human
- Entrez Gene: 15228 Mouse
- Entrez Gene: 24370 Rat
- Omim: 164874 Human
- SwissProt: P55316 Human
- SwissProt: Q60987 Mouse
- SwissProt: Q00939 Rat
- Unigene: 695962 Human
- Unigene: 708841 Human
- Unigene: 4704 Mouse
- Unigene: 9864 Rat
see all
Alternative names
- BF-1 antibody
- BF-2 antibody
- BF1 antibody
- BF2 antibody
- Brain factor 1 antibody
- Brain factor 2 antibody
- FHKL antibody
- FKH2 antibody
- FKHL1 antibody
- FKHL2 antibody
- FKHL3 antibody
- FKHL4 antibody
- Forkhead box protein G1 antibody
- Forkhead box protein G1A antibody
- Forkhead box protein G1B antibody
- Forkhead box protein G1C antibody
- Forkhead like 1 antibody
- Forkhead like 2 antibody
- Forkhead like 3 antibody
- Forkhead like 4 antibody
- Forkhead-related protein FKHL1 antibody
- Forkhead-related protein FKHL2 antibody
- Forkhead-related protein FKHL3 antibody
- FOXG1 antibody
- FOXG1_HUMAN antibody
- FOXG1A antibody
- FOXG1B antibody
- FOXG1C antibody
- HBF 1 antibody
- HBF G2 antibody
- hBF-2 antibody
- HBF2 antibody
- HFK1 antibody
- HFK2 antibody
- HFK3 antibody
- KHL2 antibody
- Oncogene QIN antibody
- QIN antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] (ab196868)

Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor cell line) cells labeling FOXG1 with ab196868 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on C6 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

Immunohistochemical analysis of paraffin-embedded mouse E14 tissue labeling FOXG1 with ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 mouse is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen mouse embryo E14.5 tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] (ab196868)

FOXG1 was immunoprecipitated from 0.35 mg mouse brain lysate 10 µg with ab196868 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab196868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: mouse brain lysate 10 µg

Lane 2: ab196868 IP in mouse brain cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196868 in mouse brain lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 32s
Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868)

All lanes : Anti-FOXG1 antibody [EPR18987] (ab196868) at 1/1000 dilution

Lane 1 : Rat E18 brain lysate
Lane 2 : Mouse E12.5 brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 52 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with the literature (PMID: 26508630). The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [EPR18987] (ab196868)

Intracellular flow cytometric analysis of4% paraformaldehyde fixed, 90% methanol permeabilized C6 (rat glial tumor cell line)cell line labeling FOXG1 with ab196868 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

Immunohistochemical analysis of paraffin-embedded rat E14 tissue labeling FOXG1 with ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 rat is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in cells from a human glioma (PMID: 28465359) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen rat embryo E14.5 tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868)

All lanes : Anti-FOXG1 antibody [EPR18987] (ab196868) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate
Lane 5 : Mouse brain lysate
Lane 6 : Mouse heart lysate
Lane 7 : Mouse kidney lysate
Lane 8 : Mouse spleen lysate
Lane 9 : Rat brain lysate
Lane 10 : Rat heart lysate
Lane 11 : Rat liver lysate
Lane 12 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 52 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time : Lanes 1-4: 15 seconds; Lanes 5-12: 3 minutes.

This target is expressed during brain development (PMID: 26896590, PMID: 7959731). The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
Anti-FOXG1 antibody [EPR18987] (ab196868)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab196868? Please let us know so that we can cite the reference in this datasheet.

ab196868 has been referenced in 6 publications.

Miyoshi G et al. FoxG1 regulates the formation of cortical GABAergic circuit during an early postnatal critical period resulting in autism spectrum disorder-like phenotypes. Nat Commun 12:3773 (2021). WB ; Mouse . PubMed: 34145239
Zhao X et al. GDF-5 promotes epidermal stem cells proliferation via Foxg1-cyclin D1 signaling. Stem Cell Res Ther 12:42 (2021). PubMed: 33413682
Li X et al. CircRNA circ_0067772 aggravates the malignant progression of cutaneous squamous cell carcinoma by regulating miR-1238-3p/FOXG1 axis. Genes Genomics 43:491-501 (2021). PubMed: 33709381
Xi H et al. FOXG1 improves mitochondrial function and promotes the progression of nasopharyngeal carcinoma. Mol Med Rep 24:N/A (2021). PubMed: 34278485
Zhang C & Wu S microRNA -378a-3p Restrains the Proliferation of Retinoblastoma Cells but Promotes Apoptosis of Retinoblastoma Cells via Inhibition of FOXG1. Invest Ophthalmol Vis Sci 61:31 (2020). PubMed: 32428232
Velasco S et al. Individual brain organoids reproducibly form cell diversity of the human cerebral cortex. Nature 570:523-527 (2019). PubMed: 31168097

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (organoid)

Permeabilization

Yes - 0.3%tritonX-100

Specification

organoid

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 26 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (6)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-FOXG1 antibody [EPR18987]