Recombinant
RabMAb

Recombinant Anti-FOXG1 antibody [EPR18987] (ab196868)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18987] to FOXG1
  • Suitable for: WB, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FOXG1 antibody [EPR18987]
    See all FOXG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18987] to FOXG1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human FOXG1 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: P55316

  • Positive control

    • WB: Mouse E12.5 brain lysate; Rat E18 brain lysate; Human fetal brain lysate; Mouse brain lysate; Rat brain lysate. IHC-P: Human glioma tissue; Mouse and rat E14 tissues. IHC-Fr: Mouse and rat embryo E14.5 tissues. ICC/IF: C6 cells. Flow Cytometry: C6 cells. IP: Mouse brain cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab196868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
Flow Cyt 1/60.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For mouse and rat samples we recommend a dilution of 1/2000.
IHC-Fr 1/500.
ICC/IF 1/100.
IP 1/30.

Target

  • Function

    Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.

  • Tissue specificity

    Expression is restricted to the neurons of the developing telencephalon.

  • Involvement in disease

    Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.

  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BF-1 antibody
    • BF-2 antibody
    • BF1 antibody
    • BF2 antibody
    • Brain factor 1 antibody
    • Brain factor 2 antibody
    • FHKL antibody
    • FKH2 antibody
    • FKHL1 antibody
    • FKHL2 antibody
    • FKHL3 antibody
    • FKHL4 antibody
    • Forkhead box protein G1 antibody
    • Forkhead box protein G1A antibody
    • Forkhead box protein G1B antibody
    • Forkhead box protein G1C antibody
    • Forkhead like 1 antibody
    • Forkhead like 2 antibody
    • Forkhead like 3 antibody
    • Forkhead like 4 antibody
    • Forkhead-related protein FKHL1 antibody
    • Forkhead-related protein FKHL2 antibody
    • Forkhead-related protein FKHL3 antibody
    • FOXG1 antibody
    • FOXG1_HUMAN antibody
    • FOXG1A antibody
    • FOXG1B antibody
    • FOXG1C antibody
    • HBF 1 antibody
    • HBF G2 antibody
    • hBF-2 antibody
    • HBF2 antibody
    • HFK1 antibody
    • HFK2 antibody
    • HFK3 antibody
    • KHL2 antibody
    • Oncogene QIN antibody
    • QIN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] (ab196868)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor cell line) cells labeling FOXG1 with ab196868 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on C6 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunohistochemical analysis of paraffin-embedded mouse E14 tissue labeling FOXG1 with ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 mouse is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)
    Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen mouse embryo E14.5 tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

  • Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] (ab196868)
    Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] (ab196868)

    FOXG1 was immunoprecipitated from 0.35 mg mouse brain lysate 10 µg with ab196868 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab196868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: mouse brain lysate 10 µg

    Lane 2: ab196868 IP in mouse brain cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196868 in mouse brain lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32s

     

  • Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868)
    Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868)
    All lanes : Anti-FOXG1 antibody [EPR18987] (ab196868) at 1/1000 dilution

    Lane 1 : Rat E18 brain lysate
    Lane 2 : Mouse E12.5 brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with the literature (PMID: 26508630). The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Flow Cytometry - Anti-FOXG1 antibody [EPR18987] (ab196868)
    Flow Cytometry - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C6 (rat glial tumor cell line) cell line labeling FOXG1 with ab196868 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunohistochemical analysis of paraffin-embedded rat E14 tissue labeling FOXG1 with ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 rat is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in cells from a human glioma (PMID: 28465359) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)
    Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] (ab196868)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen rat embryo E14.5 tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

  • Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868)
    Western blot - Anti-FOXG1 antibody [EPR18987] (ab196868)
    All lanes : Anti-FOXG1 antibody [EPR18987] (ab196868) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Mouse heart lysate
    Lane 7 : Mouse kidney lysate
    Lane 8 : Mouse spleen lysate
    Lane 9 : Rat brain lysate
    Lane 10 : Rat heart lysate
    Lane 11 : Rat liver lysate
    Lane 12 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time : Lanes 1-4: 15 seconds; Lanes 5-12: 3 minutes.

    This target is expressed during brain development (PMID: 26896590, PMID: 7959731). The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab196868? Please let us know so that we can cite the reference in this datasheet.

ab196868 has not yet been referenced specifically in any publications.

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