RecombinantRabMAb

Recombinant Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

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Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
  • Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
  • Flow Cytometry - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
  • Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18987] to FOXG1 - BSA and Azide free
  • Suitable for: IP, WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FOXG1 antibody [EPR18987] - BSA and Azide free
    See all FOXG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18987] to FOXG1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human FOXG1 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: P55316

  • Positive control

    • IHC-P: Mouse E14 brain tissue. IP: Mouse brain lysate.

  • General notes

    Ab227888 is the carrier-free version of ab196868. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227888 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab227888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.

  • Tissue specificity

    Expression is restricted to the neurons of the developing telencephalon.

  • Involvement in disease

    Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.

  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BF-1 antibody
    • BF-2 antibody
    • BF1 antibody
    • BF2 antibody
    • Brain factor 1 antibody
    • Brain factor 2 antibody
    • FHKL antibody
    • FKH2 antibody
    • FKHL1 antibody
    • FKHL2 antibody
    • FKHL3 antibody
    • FKHL4 antibody
    • Forkhead box protein G1 antibody
    • Forkhead box protein G1A antibody
    • Forkhead box protein G1B antibody
    • Forkhead box protein G1C antibody
    • Forkhead like 1 antibody
    • Forkhead like 2 antibody
    • Forkhead like 3 antibody
    • Forkhead like 4 antibody
    • Forkhead-related protein FKHL1 antibody
    • Forkhead-related protein FKHL2 antibody
    • Forkhead-related protein FKHL3 antibody
    • FOXG1 antibody
    • FOXG1_HUMAN antibody
    • FOXG1A antibody
    • FOXG1B antibody
    • FOXG1C antibody
    • HBF 1 antibody
    • HBF G2 antibody
    • hBF-2 antibody
    • HBF2 antibody
    • HFK1 antibody
    • HFK2 antibody
    • HFK3 antibody
    • KHL2 antibody
    • Oncogene QIN antibody
    • QIN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor cell line) cells labeling FOXG1 with ab196868 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on C6 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of paraffin-embedded mouse E14 tissue labeling FOXG1 with ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 mouse is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen mouse embryo E14.5 tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

  • Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunoprecipitation - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    FOXG1 was immunoprecipitated from 0.35 mg mouse brain lysate 10 µg with ab196868 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab196868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: mouse brain lysate 10 µg

    Lane 2: ab196868 IP in mouse brain cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196868 in mouse brain lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32s

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

  • Flow Cytometry - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Flow Cytometry - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C6 (rat glial tumor cell line) cell line labeling FOXG1 with ab196868 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of paraffin-embedded rat E14 tissue labeling FOXG1 with ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 rat is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in cells from a human glioma (PMID: 28465359) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen rat embryo E14.5 tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

  • Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab227888? Please let us know so that we can cite the reference in this datasheet.

ab227888 has not yet been referenced specifically in any publications.

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