Recombinant
RabMAb

Recombinant Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

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Overview

  • Product name

    Anti-FOXG1 antibody [EPR18987] - BSA and Azide free
    See all FOXG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18987] to FOXG1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human FOXG1 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: P55316

  • Positive control

    • IHC-P: Mouse E14 brain tissue.

  • General notes

    Ab227888 is the carrier-free version of ab196868. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227888 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab227888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.

  • Tissue specificity

    Expression is restricted to the neurons of the developing telencephalon.

  • Involvement in disease

    Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.

  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BF-1 antibody
    • BF-2 antibody
    • BF1 antibody
    • BF2 antibody
    • Brain factor 1 antibody
    • Brain factor 2 antibody
    • FHKL antibody
    • FKH2 antibody
    • FKHL1 antibody
    • FKHL2 antibody
    • FKHL3 antibody
    • FKHL4 antibody
    • Forkhead box protein G1 antibody
    • Forkhead box protein G1A antibody
    • Forkhead box protein G1B antibody
    • Forkhead box protein G1C antibody
    • Forkhead like 1 antibody
    • Forkhead like 2 antibody
    • Forkhead like 3 antibody
    • Forkhead like 4 antibody
    • Forkhead-related protein FKHL1 antibody
    • Forkhead-related protein FKHL2 antibody
    • Forkhead-related protein FKHL3 antibody
    • FOXG1 antibody
    • FOXG1_HUMAN antibody
    • FOXG1A antibody
    • FOXG1B antibody
    • FOXG1C antibody
    • HBF 1 antibody
    • HBF G2 antibody
    • hBF-2 antibody
    • HBF2 antibody
    • HFK1 antibody
    • HFK2 antibody
    • HFK3 antibody
    • KHL2 antibody
    • Oncogene QIN antibody
    • QIN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in cells from a human glioma (PMID: 28465359) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of paraffin-embedded rat E14 tissue labeling FOXG1 with ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 rat is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen mouse embryo E14.5 tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

  • Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunohistochemistry (Frozen sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen rat embryo E14.5 tissue labeling FOXG1 with ab196868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining in the cortical plate of the telencephalon (LV: lateral ventricle; PMID: 14704420) is observed. Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor cell line) cells labeling FOXG1 with ab196868 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on C6 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

  • Flow Cytometry - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Flow Cytometry - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C6 (rat glial tumor cell line) cell line labeling FOXG1 with ab196868 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (ab227888)

    Immunohistochemical analysis of paraffin-embedded mouse E14 tissue labeling FOXG1 with ab196868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on cerebrum and olfactory epithelium of E14 mouse is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196868).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab227888 has not yet been referenced specifically in any publications.

Publishing research using ab227888? Please let us know so that we can cite the reference in this datasheet.

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