Key features and details
- Mouse monoclonal [mAbcam 51774] to FOXG1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-FOXG1 antibody [mAbcam 51774]
See all FOXG1 primary antibodies
DescriptionMouse monoclonal [mAbcam 51774] to FOXG1
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Chicken, Cow, Zebrafish
- This antibody gave a positive signal in human brain tissue lysate (ab29466).
This antibody clone is manufactured by Abcam.
We can conjugate this antibody to FITC for you (please see ab150200 for details).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Clone numbermAbcam 51774
Our Abpromise guarantee covers the use of ab51774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionTranscription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
Tissue specificityExpression is restricted to the neurons of the developing telencephalon.
Involvement in diseaseDefects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
- Information by UniProt
- BF-1 antibody
- BF-2 antibody
- BF1 antibody
Anti-FOXG1 antibody [mAbcam 51774] (ab51774) at 5 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Overlay histogram showing SHSY-5Y cells stained with ab51774 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51774, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab51774 has not yet been referenced specifically in any publications.