Overview

  • Product name
    Anti-FOXG1 antibody [mAbcam 51774]
    See all FOXG1 primary antibodies
  • Description
    Mouse monoclonal [mAbcam 51774] to FOXG1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Chicken, Cow, Zebrafish
  • Immunogen

    Synthetic peptide within Human FOXG1 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. Peptide available as ab19644.
    Database link: P55316
    (Peptide available as ab19644)

  • Positive control
    • This antibody gave a positive signal in human brain tissue lysate (ab29466).
  • General notes

    This antibody clone is manufactured by Abcam.

    We can conjugate this antibody to FITC for you (please see ab150200 for details).

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab51774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Flow Cyt Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
  • Tissue specificity
    Expression is restricted to the neurons of the developing telencephalon.
  • Involvement in disease
    Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BF-1 antibody
    • BF-2 antibody
    • BF1 antibody
    • BF2 antibody
    • Brain factor 1 antibody
    • Brain factor 2 antibody
    • FHKL antibody
    • FKH2 antibody
    • FKHL1 antibody
    • FKHL2 antibody
    • FKHL3 antibody
    • FKHL4 antibody
    • Forkhead box protein G1 antibody
    • Forkhead box protein G1A antibody
    • Forkhead box protein G1B antibody
    • Forkhead box protein G1C antibody
    • Forkhead like 1 antibody
    • Forkhead like 2 antibody
    • Forkhead like 3 antibody
    • Forkhead like 4 antibody
    • Forkhead-related protein FKHL1 antibody
    • Forkhead-related protein FKHL2 antibody
    • Forkhead-related protein FKHL3 antibody
    • FOXG1 antibody
    • FOXG1_HUMAN antibody
    • FOXG1A antibody
    • FOXG1B antibody
    • FOXG1C antibody
    • HBF 1 antibody
    • HBF G2 antibody
    • hBF-2 antibody
    • HBF2 antibody
    • HFK1 antibody
    • HFK2 antibody
    • HFK3 antibody
    • KHL2 antibody
    • Oncogene QIN antibody
    • QIN antibody
    see all

Images

  • Anti-FOXG1 antibody [mAbcam 51774] (ab51774) at 5 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/2000 dilution

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

  • Overlay histogram showing SHSY-5Y cells stained with ab51774 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51774, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

ab51774 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

Unfortunately ab51774 has not been tested either with Chicken, nor IHC-P. It doesn’t mean it won’t work in this species / application, but we don’t have data to support it.

However, due to sequence homology, the antibody is very likely to cross react with Chicken. If you are interested in participating in our antibodies evaluation system (Abreview), and test ab51774 with IH –P and / or chicken species (by WB or Flow Cytometry) I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.

Therefore, I can offer a discount off a future purchase if you buy an antibody and test it in a new species or with a new application and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab51774 in IHC-P and / or chicken species. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

3. Test it in the new species / application.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).

To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: http://ops.adminsite.com/admin/crm2/www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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