Overview

  • Product name

  • Description

    Rabbit polyclonal to FOXH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human FOXH1 (internal sequence). The exact sequence is proprietary.
    Database link: O75593

  • Positive control

    • WB: Raji, NCI-H929, HCT 116, NCI-H1299 and A549 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab229241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.

Target

  • Function

    Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Domain

    The FM region is required for binding SMAD2/SMAD4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated SMAD2 that is in an activated SMAD complex.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FAST 1 antibody
    • Fast 2 antibody
    • Fast-1 antibody
    • Fast-2 antibody
    • FAST1 antibody
    • Fast2 antibody
    • Forkhead activin signal transducer 1 antibody
    • Forkhead activin signal transducer 2 antibody
    • Forkhead box H1 antibody
    • Forkhead box protein H1 antibody
    • FOX H1 antibody
    • FOXH 1 antibody
    • FOXH1 antibody
    • FOXH1_HUMAN antibody
    • hFAST 1 antibody
    • hFAST-1 antibody
    • hFAST1 antibody
    • Human homolog of Xenopus forkhead activin signal transducer 1 antibody
    • TGF beta/Activin signal transducer FAST1 antibody
    see all

Images

  • All lanes : Anti-FOXH1 antibody (ab229241) at 1/500 dilution

    Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : NCI-H929 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 39 kDa



    10% SDS-PAGE

  • All lanes : Anti-FOXH1 antibody (ab229241) at 1/1000 dilution

    Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 2 : NCI-H1299 whole cell lysate
    Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 39 kDa



    10% SDS-PAGE

References

ab229241 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229241.
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