Overview

  • Product name

  • Description

    Rabbit polyclonal to FOXH1
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes Forkhead box protein H1 (FOXH1)
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    A region within synthetic peptide: GPSSSSETPL WPLCSLPGPT IIEGESSQGE VIRPSPVTPD QGSWPLHLLE, corresponding to amino acids 241-290 of Mouse FOXH1

  • Positive control

    • SP2/0 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Domain

    The FM region is required for binding SMAD2/SMAD4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated SMAD2 that is in an activated SMAD complex.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FAST 1 antibody
    • Fast 2 antibody
    • Fast-1 antibody
    • Fast-2 antibody
    • FAST1 antibody
    • Fast2 antibody
    • Forkhead activin signal transducer 1 antibody
    • Forkhead activin signal transducer 2 antibody
    • Forkhead box H1 antibody
    • Forkhead box protein H1 antibody
    • FOX H1 antibody
    • FOXH 1 antibody
    • FOXH1 antibody
    • FOXH1_HUMAN antibody
    • hFAST 1 antibody
    • hFAST-1 antibody
    • hFAST1 antibody
    • Human homolog of Xenopus forkhead activin signal transducer 1 antibody
    • TGF beta/Activin signal transducer FAST1 antibody
    see all

Images

  • Anti-FOXH1 antibody (ab49133) at 0.5 µg/ml + SP2/0 lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 44 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 82 kDa, 90 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%

References

This product has been referenced in:

  • Halstead AM & Wright CV Disrupting Foxh1-Groucho interaction reveals robustness of nodal-based embryonic patterning. Mech Dev 136:155-65 (2015). Read more (PubMed: 25511461) »
See 1 Publication for this product

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