Key features and details
- Rabbit polyclonal to FOXH1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-FOXH1 antibody
See all FOXH1 primary antibodies
DescriptionRabbit polyclonal to FOXH1
SpecificityThis antibody recognizes Forkhead box protein H1 (FOXH1)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat
- SP2/0 lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab49133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionTranscriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).
Sequence similaritiesContains 1 fork-head DNA-binding domain.
DomainThe FM region is required for binding SMAD2/SMAD4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated SMAD2 that is in an activated SMAD complex.
- Information by UniProt
- FAST 1 antibody
- Fast 2 antibody
- Fast-1 antibody
Anti-FOXH1 antibody (ab49133) at 0.5 µg/ml + SP2/0 lysate at 10 µg with 5% skimmed milk in PBS
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 82 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
ab49133 has been referenced in 2 publications.
- Loizou E et al. A Gain-of-Function p53-Mutant Oncogene Promotes Cell Fate Plasticity and Myeloid Leukemia through the Pluripotency Factor FOXH1. Cancer Discov 9:962-979 (2019). PubMed: 31068365
- Halstead AM & Wright CV Disrupting Foxh1-Groucho interaction reveals robustness of nodal-based embryonic patterning. Mech Dev 136:155-65 (2015). PubMed: 25511461