Anti-FOXH1 antibody - N-terminal (ab189960)
Key features and details
- Rabbit polyclonal to FOXH1 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FOXH1 antibody - N-terminal
See all FOXH1 primary antibodies -
Description
Rabbit polyclonal to FOXH1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human FOXH1 (N terminal). The peptide corresponds to 19 amino acids.
Database link: O75593 -
Positive control
- Human prostate and liver tissue. Human liver lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 39 kDa.
|
|
IHC-P |
Use a concentration of 5 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 39 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
-
Function
Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Domain
The FM region is required for binding SMAD2/SMAD4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated SMAD2 that is in an activated SMAD complex. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 8928 Human
- Omim: 603621 Human
- SwissProt: O75593 Human
- Unigene: 708365 Human
-
Alternative names
- FAST 1 antibody
- Fast 2 antibody
- Fast-1 antibody
see all
Images
-
Anti-FOXH1 antibody - N-terminal (ab189960) at 1 µg/ml + human liver tissue lysate
Predicted band size: 39 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling FOXH1 with ab189960 at 5 µg/ml.
-
Immunofluorescence analysis of human liver tissue labeling FOXH1 with ab189960 at 20 µg/ml.
Protocols
Datasheets and documents
-
Datasheet download
References (2)
ab189960 has been referenced in 2 publications.
- Ouyang X et al. A comprehensive analysis of FOX family in HCC and experimental evidence to support the oncogenic role of FOXH1. Aging (Albany NY) 14:2268-2286 (2022). PubMed: 35255005
- Tian T et al. FAST1 Predicts Poor Survival of Renal Carcinoma and Promotes Its Progression Through the TGF-ß/Smad Pathway. Onco Targets Ther 14:1487-1499 (2021). PubMed: 33679133