Overview

  • Product name

  • Description

    Rabbit polyclonal to FOXI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 76-262 of Human FOXI1 (Uniprot ID: Q12951).

  • Positive control

    • Human Huh7 xenograft tissue; HeLa cells; A549, HeLa, HepG2 and HCT116 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Transcriptional activator required for the development of normal hearing, sense of balance and kidney function. Required for the expression of SLC26A4/PDS, JAG1 and COCH in a subset of epithelial cells and the development of the endolymphatic system in the inner ear. Also required for the expression of SLC4A1/AE1, SLC4A9/AE4, ATP6V1B1 and the differentiation of intercalated cells in the epithelium of distal renal tubules.
  • Tissue specificity

    Expressed in kidney.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FKH10 antibody
    • FKHL10 antibody
    • Forkhead (Drosophila) like 10 antibody
    • Forkhead box I1 antibody
    • Forkhead box protein I1 antibody
    • Forkhead like 10 antibody
    • Forkhead related activator 6 antibody
    • Forkhead related transcription factor 6 antibody
    • Forkhead-related protein FKHL10 antibody
    • Forkhead-related transcription factor 6 antibody
    • FOXI1 antibody
    • FOXI1_HUMAN antibody
    • FREAC 6 antibody
    • FREAC-6 antibody
    • FREAC6 antibody
    • Hepatocyte nuclear factor 3 forkhead homolog 3 antibody
    • HFH 3 antibody
    • HFH-3 antibody
    • HFH3 antibody
    • HNF 3/fork head homolog 3 antibody
    • HNF-3 fork-head homolog 3 antibody
    • HNF-3/fork-head homolog 3 antibody
    • Human HNF-3 fork-head homolog-3 HFH-3 mRNA complete cds antibody
    • MGC34197 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human Huh7 xenograft tissue, labeling FOXI1 with ab153935 at 1/500 dilution.
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling FOXI1 with ab153935 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Anti-FOXI1 antibody (ab153935) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 31 kDa



    12% SDS PAGE

References

ab153935 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Kidney sections)
Permeabilization
Yes - Triton x-100
Specification
Kidney sections
Blocking step
Donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde

Weam Shahin

Verified customer

Submitted May 01 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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