Overview

  • Product name

  • Description

    Rabbit polyclonal to FOXI3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 396 - 445 (EASADTLQLS NSTSNSTGQR SSYYSPFPAS TSGGQSSPFS SPFHNFSMVN) of human FOXI3 (XP_001126001).

  • Positive control

    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

Target

Images

  • Anti-FOXI3 antibody (ab81824) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 43 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?

References

ab81824 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for contacting Abcam regarding our FOXI3 antibodies. As we discussed on the phone, we do not have any antibodies against this target that have been validated for ICC.  Our catalog is constantly being updated, so I recommend checking back with us. Unfortunately, we do not have sample aliquots available, but I would like to encourage you to take advantage of our testing discount program.  - Simply reply to this email and let me know that you would like to test ab81824 in ICC and I will send you a discount code. - Purchase the antibody at full price and test it in ICC. - Submit your results in the form of an Abreview. - Once the Abreview is submitted, the discount code becomes activated and can be redeemed for one free primary antibody of your choice. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.  

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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