Overview

  • Product name

    Anti-FoxJ1 antibody [EPR21874]
    See all FoxJ1 primary antibodies
  • Description

    Rabbit monoclonal [EPR21874] to FoxJ1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human FoxJ1 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q92949

  • Positive control

    • IHC-P: Human choroid plexus papilloma tissue. Mouse fallopian tube and rat lung tissue.
  • General notes

     This product was previously labelled as HFH4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    May play an important role in cell fate determination during lung development and in spermatogenesis.
  • Tissue specificity

    Testis, oviduct, lung and brain cortex.
  • Involvement in disease

    Genetic variations in FOXJ1 may be associated with susceptibility to allergic rhinitis (ALRH) [MIM:607154]. Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Developmental stage

    Expressed in developing lung, kidney and central nervous system.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FKHL13 antibody
    • Forkhead box J1 antibody
    • Forkhead box protein J1 antibody
    • Forkhead Homologue 4 antibody
    • Forkhead related protein FKHL13 antibody
    • Forkhead-related protein FKHL13 antibody
    • Fox J1 antibody
    • Foxj1 antibody
    • FOXJ1_HUMAN antibody
    • Hepatocyte nuclear factor 3 forkhead homolog 4 antibody
    • HFH 4 antibody
    • HFH-4 antibody
    • HFH4 antibody
    • HNF 3/forkhead homolog 4 antibody
    • MGC35202 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human choroid plexus papilloma tissue labeling FoxJ1 with ab235445 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human choroid plexus papilloma (PMID: 28719464; PMID: 26690880) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human pinealoma tissue labeling FoxJ1 with ab235445 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control: No staining in human pinealoma (PMID: 28719464) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue labeling FoxJ1 with ab235445 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse fallopian tube (PMID: 26690880; PMID: 27562488) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling FoxJ1 with ab235445 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in bronchiole of rat lung (PMID: 26690880; PMID: 19502490) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

References

ab235445 has not yet been referenced specifically in any publications.

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