Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXJ2
  • Host species
    Rabbit
  • Specificity
    ab22857 reacts with Forkhead box protein J2 (FOXJ2).
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    A region within synthetic peptide: AGASGRESAE GPPPLYNTNH DFKFSYSEIN FQDLSWSFRN LYKSMLEKSS, corresponding to internal sequence amino acids 218-267 of Human FOXJ2

  • Positive control
    • HepG2 cell lysate and human stomach

Properties

Applications

Our Abpromise guarantee covers the use of ab22857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Transcriptional activator. Able to bind to two different type of DNA binding sites. Isoform FOXJ2.L behaves as a more potent transactivator than FOXJ2.S.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FHX antibody
    • Fork head homologous X antibody
    • Forkhead box J2 antibody
    • Forkhead box protein J2 antibody
    • FOXJ2 antibody
    • FOXJ2_HUMAN antibody
    see all

Images

  • Anti-FOXJ2 antibody (ab22857) at 2 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • ab22857, at 4 µg/ml, staining human FOXJ2 in human Stomach by Immunohistochemistry, Paraffin embedded tissue. Positive stained cells, indicated with arrows, are epithelial cells of fundic gland. Magnification 400 x.

References

ab22857 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry.

We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

I have conducted a search for you and found the following antibodies:

FOXJ2 - 2 antibodies but not tested yet in Rat (suitable for Western blot)

ab22857, ab49160

HAND1 - 4 antibodies but not tested yet in Rat (suitable for Western blot)

ab46822, ab52767, ab86009, ab104320



NFAT5

ab3446 - reacts with Rat

ab105886 - predicted to react with Rat





Nkx2-3

ab49162 - predicted to react with Rat



ab66366- predicted to react with Rat





Nkx2-4

ab48331 - predicted to react with Rat



ab49162 - predicted to react with Rat



ab66366 - predicted to react with Rat


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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