• Product name

  • Description

    Rabbit polyclonal to FOXK1/MNF
  • Host species

  • Tested applications

    Suitable for: WB, ChIP, IP, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human FOXK1/MNF aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab19709, ab19710)

  • Positive control

    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell; HeLa Nuclear; HepG2 Nuclear; Jurkat Whole Cell; Jurkat Nuclear; K562 Whole Cell and SW480 Whole Cell
  • General notes

    Previously labelled as FOXK1. 


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab18196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 20053746
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.


  • Function

    Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli.
  • Tissue specificity

    Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node.
  • Sequence similarities

    Contains 1 FHA domain.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • A630048H08Rik antibody
    • AI463295 antibody
    • ENSMUSG00000075577 antibody
    • FHX antibody
    • Fork head antibody
    • Forkhead box protein K1 antibody
    • FOXJ2 forkhead factor antibody
    • FOXK1 antibody
    • FOXK1_HUMAN antibody
    • Gm10868 antibody
    • MNF antibody
    • Myocyte nuclear factor antibody
    see all


  • All lanes : Anti-FOXK1/MNF antibody (ab18196) at 1/5000 dilution

    Lane 1 : Human H1299 cell lysate
    Lane 2 : Mouse embryonic stem cell lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

    Exposure time: 1 minute

    See Abreview

  • Image courtesy of Human Protein Atlas

    ab18196 staining in human rectum with colo-rectal cancer, showing staining of the tumour cells (in brown). Paraffin embedded rectal tissue was incubated with ab18196 (1:1000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab18196 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.

  • All lanes : Anti-FOXK1/MNF antibody (ab18196) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : Jurkat nuclear extract lysate (ab14844)
    Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 7 : SW480 whole cell lysate (ab3957)

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 95 kDa why is the actual band size different from the predicted?

    Exposure time: 5 minutes
  • ICC/IF image of ab18196 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18196, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • FOXK1/MNF - ChIP Grade was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to FOXK1/MNF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18196.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 96kDa: FOXK1 / MNF.


This product has been referenced in:

  • Sakaguchi M  et al. FoxK1 and FoxK2 in insulin regulation of cellular and mitochondrial metabolism. Nat Commun 10:1582 (2019). Read more (PubMed: 30952843) »
  • Xu H  et al. FOXK1 promotes glioblastoma proliferation and metastasis through activation of Snail transcription. Exp Ther Med 15:3108-3116 (2018). Read more (PubMed: 29456714) »
See all 7 Publications for this product

Customer reviews and Q&As

Western blot
Mouse Cell lysate - whole cell (ES cell)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE gel)
Loading amount
20 µg
ES cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jul 02 2013

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