Anti-FOXK1/MNF antibody (ab18196)
Key features and details
- Rabbit polyclonal to FOXK1/MNF
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FOXK1/MNF antibody
See all FOXK1/MNF primary antibodies -
Description
Rabbit polyclonal to FOXK1/MNF -
Host species
Rabbit -
Specificity
Replenishment batches of our polyclonal antibody, ab18196 are tested in WB. Previous batches were additionally validated in ICC/IF and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab309510.
-
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000.
|
IP |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use a concentration of 1 µg/ml.
|
Notes |
---|
WB
1/1000. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
-
Function
Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli. -
Tissue specificity
Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node. -
Sequence similarities
Contains 1 FHA domain.
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 221937 Human
- Entrez Gene: 17425 Mouse
- SwissProt: P85037 Human
- SwissProt: P42128 Mouse
- Unigene: 487393 Human
- Unigene: 24214 Mouse
-
Alternative names
- A630048H08Rik antibody
- AI463295 antibody
- ENSMUSG00000075577 antibody
see all
Images
-
All lanes : Anti-FOXK1/MNF antibody (ab18196) at 1/5000 dilution
Lane 1 : Human H1299 cell lysate
Lane 2 : Mouse embryonic stem cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
-
All lanes : Anti-FOXK1/MNF antibody (ab18196) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : Jurkat nuclear extract lysate (ab14844)
Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 7 :SW480 whole cell lysate (ab3957)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes -
ICC/IF image of ab18196 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18196, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
FOXK1/MNF - ChIP Grade was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to FOXK1/MNF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18196.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 96kDa: FOXK1 / MNF.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (24)
ab18196 has been referenced in 24 publications.
- Zhang F et al. Circular RNA Eps15-homology domain containing protein 2 motivates proliferation, glycolysis but refrains autophagy in non-small cell lung cancer via crosstalk with microRNA-3186-3p and forkhead box K1. Bioengineered 13:6464-6475 (2022). PubMed: 35220908
- Chong YK et al. Cyclin J-CDK complexes limit innate immune responses by reducing proinflammatory changes in macrophage metabolism. Sci Signal 15:eabm5011 (2022). PubMed: 35412849
- Huang X et al. microRNA-16-5p suppresses cell proliferation and angiogenesis in colorectal cancer by negatively regulating forkhead box K1 to block the PI3K/Akt/mTOR pathway. Eur J Histochem 66:N/A (2022). PubMed: 35536149
- Li C et al. Natural antisense RNA Foxk1-AS promotes myogenic differentiation by inhibiting Foxk1 activity. Cell Commun Signal 20:77 (2022). PubMed: 35642035
- Chen W et al. circ-PRKCI targets miR-1294 and miR-186-5p by downregulating FOXK1 expression to suppress glycolysis in hepatocellular carcinoma. Mol Med Rep 23:N/A (2021). PubMed: 33880589