Key features and details
- Rabbit polyclonal to FOXK1/MNF
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FOXK1/MNF antibody
See all FOXK1/MNF primary antibodies
DescriptionRabbit polyclonal to FOXK1/MNF
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide corresponding to Human FOXK1/MNF aa 683-733.
Database link: NP_001032242.1
- Whole cell lysate from HeLa cells
Previously labelled as FOXK1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85999 was affinity purified using an epitope specific to FOXK1 / MNF immobilized on solid support.
Our Abpromise guarantee covers the use of ab85999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 75 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli.
Tissue specificityExpressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node.
Sequence similaritiesContains 1 FHA domain.
Contains 1 fork-head DNA-binding domain.
- Information by UniProt
- A630048H08Rik antibody
- AI463295 antibody
- ENSMUSG00000075577 antibody
All lanes : Anti-FOXK1/MNF antibody (ab85999) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling FOXK1/MNF with ab85999 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
Detection of FOXK1/MNF by Western blot of Immunprecipitate.
ab85999, at 1 µg/ml, staining FOXK1 / MNF in HeLa whole cell lysate immunoprecipitated using ab85999 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 seconds.
All lanes : Anti-FOXK1/MNF antibody (ab85999) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : Control IgG
ab85999 has not yet been referenced specifically in any publications.