Anti-FOXL2 antibody (ab5096)
Key features and details
- Goat polyclonal to FOXL2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-FOXL2 antibody
See all FOXL2 primary antibodies -
Description
Goat polyclonal to FOXL2 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide corresponding to FOXL2 aa 364-376 (C terminal).
Sequence:DSKTGALHSRLDL
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Positive control
- WB: K562 and HeLa cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45-50 kDa (predicted molecular weight: 43 kDa).
Primary incubation 1 hour at room temperature. |
| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45-50 kDa (predicted molecular weight: 43 kDa). Primary incubation 1 hour at room temperature. |
Target
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Function
Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. -
Tissue specificity
In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary. -
Involvement in disease
Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Post-translational
modificationsSumoylated by SUMO1; sumoylation is required for transcriptional repression activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 281770 Cow
- Entrez Gene: 668 Human
- Entrez Gene: 26927 Mouse
- Entrez Gene: 367152 Rat
- Omim: 605597 Human
- SwissProt: Q6VFT7 Cow
- SwissProt: P58012 Human
- SwissProt: O88470 Mouse
see all -
Alternative names
- Blepharophimosis antibody
- Blepharophimosis epicanthus inversus and ptosis 1 antibody
- Blepharophimosis epicanthus inversus and ptosis antibody
see all
Images
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Western blot - Anti-FOXL2 antibody (ab5096)All lanes : Anti-FOXL2 antibody (ab5096) at 2 µg/ml
Lane 1 : K562 cell lysate, 35µg protein in RIPA buffer
Lanes 2 & 4 : K562 cell lysate incubated with immunizing peptide, 35µg protein in RIPA buffer
Lane 3 : HeLa cell lysate, 35µg protein in RIPA buffer
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Primary incubation 1 hour at room temperature.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (49)
ab5096 has been referenced in 49 publications.
- Heng D et al. Mtor inhibition by INK128 extends functions of the ovary reconstituted from germline stem cells in aging and premature aging mice. Aging Cell 20:e13304 (2021). PubMed: 33448083
- Frost ER et al. Two alternative methods for the retrieval of somatic cell populations from the mouse ovary. Mol Hum Reprod 27:N/A (2021). PubMed: 33973015
- Habara O et al. WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility. Development 148:N/A (2021). PubMed: 33914868
- Li R et al. Increased FOXL2 expression alters uterine structures and functions†. Biol Reprod 103:951-965 (2020). PubMed: 32948877
- Pierini S et al. Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target. JCI Insight 5:N/A (2020). PubMed: 32814714