Anti-FOXL2 antibody (ab5096)
- Datasheet
- References (27)
- Protocols
Overview
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Product nameAnti-FOXL2 antibody
See all FOXL2 primary antibodies -
DescriptionGoat polyclonal to FOXL2
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Host speciesGoat
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Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Goat, Cow
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Immunogen
Synthetic peptide:
DSKTGALHSRLDL
, corresponding to amino acids 364-376 of FOX L2. -
Positive control
- IHC-P: Human ovary tissue. WB: Human ovary, mouse ovary and human heart tissue lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab5096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45-50 kDa (predicted molecular weight: 43 kDa). |
Target
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FunctionTranscriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
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Tissue specificityIn addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
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Involvement in diseaseDefects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
Sequence similaritiesContains 1 fork-head DNA-binding domain.
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Post-translational
modificationsSumoylated by SUMO1; sumoylation is required for transcriptional repression activity. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 281770 Cow
- Entrez Gene: 668 Human
- Entrez Gene: 26927 Mouse
- Entrez Gene: 367152 Rat
- Omim: 605597 Human
- SwissProt: Q6VFT7 Cow
- SwissProt: P58012 Human
- SwissProt: O88470 Mouse
see all -
Alternative names
- Blepharophimosis antibody
- Blepharophimosis epicanthus inversus and ptosis 1 antibody
- Blepharophimosis epicanthus inversus and ptosis antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody (ab5096)Immunohistochemical analysis of paraffin embedded Human Ovary tissue labeling FOXL2 with ab5096 at 2 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.
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Western blot - Anti-FOXL2 antibody (ab5096)All lanes : Anti-FOXL2 antibody (ab5096) at 1 µg/ml
Lane 1 : Human ovary lysate (RIPA Buffer)
Lane 2 : Mouse ovary lysate (RIPA Buffer)
Lysates/proteins at 35 µg/ml per lane.
Predicted band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody (ab5096)Image from Dr CR Nicholas et al, BMC Dev Biol. 2010 Jan 8;10:2, Fig 4.ab5096 staining FOXL2 in female mice oocytes by Immunohistochemistry (PFA-fixed paraffin-embedded sections).
Grafts were fixed overnight in 4% paraformaldehyde, embedded in paraffin, and sectioned in 5 to 8 µm intervals. In brief, sections on slides were de-paraffinized, re-hydrated, antigens unmasked by incubating in target retrieval solution at 95°C for 30 minutes, permeabilized in 0.1% Triton-X100 for 5 minutes, blocked with 10% chicken serum in TBST overnight, and incubated with primary antibody ab5096 at a 1/100, in TBST with 1% serum for 1 hour at room temperature. After washing in TBST, slides were incubated with secondary antibody at 1/1000, for 30 minutes at room temperature. Cover slips were mounted with Prolong Gold Antifade with DAPI.
Ovarian cord structure was examined by TRA98 germ cell and FOXL2 granulosa somatic cell immunofluorescence over 12 days of e12.5 female genital ridge transplantation. Oocytes and granulosa cells clustered together in defi -
Western blot - Anti-FOXL2 antibody (ab5096)Anti-FOXL2 antibody (ab5096) at 0.5 µg/ml + Human heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Protocols
References
This product has been referenced in:
- Yamamoto A et al. The mechanisms underlying the effects of AMH on Müllerian duct regression in male mice. J Vet Med Sci 80:557-567 (2018). Read more (PubMed: 29526868) »
- Nakamura T et al. Ovarian mast cells migrate toward ovary-fimbria connection in neonatal MRL/MpJ mice. PLoS One 13:e0196364 (2018). Read more (PubMed: 29684078) »
