Overview

  • Product name
  • Description
    Goat polyclonal to FOXL2
  • Host species
    Goat
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Goat, Cow
  • Immunogen

    Synthetic peptide:

    DSKTGALHSRLDL

    , corresponding to amino acids 364-376 of FOX L2.

  • Positive control
    • IHC-P: Human ovary tissue. WB: Human ovary, mouse ovary and human heart tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab5096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45-50 kDa (predicted molecular weight: 43 kDa).

Target

  • Function
    Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
  • Tissue specificity
    In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
  • Involvement in disease
    Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
    Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated by SUMO1; sumoylation is required for transcriptional repression activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blepharophimosis antibody
    • Blepharophimosis epicanthus inversus and ptosis 1 antibody
    • Blepharophimosis epicanthus inversus and ptosis antibody
    • BPES 1 antibody
    • BPES antibody
    • BPES1 antibody
    • Epicanthus inversus and ptosis 1 antibody
    • Forkhead box L2 antibody
    • Forkhead box protein L2 antibody
    • Forkhead transcription factor FOXL2 antibody
    • FOX L2 antibody
    • FOXL 2 antibody
    • FOXL2 antibody
    • FOXL2_HUMAN antibody
    • PFRK antibody
    • PINTO antibody
    • PITUITARY FORKHEAD FACTOR antibody
    • POF 3 antibody
    • POF3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human Ovary tissue labeling FOXL2 with ab5096 at 2 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.

  • All lanes : Anti-FOXL2 antibody (ab5096) at 1 µg/ml

    Lane 1 : Human ovary lysate (RIPA Buffer)
    Lane 2 : Mouse ovary lysate (RIPA Buffer)

    Lysates/proteins at 35 µg/ml per lane.

    Predicted band size: 43 kDa

  • ab5096 staining FOXL2 in female mice oocytes by Immunohistochemistry (PFA-fixed paraffin-embedded sections).
    Grafts were fixed overnight in 4% paraformaldehyde, embedded in paraffin, and sectioned in 5 to 8 µm intervals. In brief, sections on slides were de-paraffinized, re-hydrated, antigens unmasked by incubating in target retrieval solution at 95°C for 30 minutes, permeabilized in 0.1% Triton-X100 for 5 minutes, blocked with 10% chicken serum in TBST overnight, and incubated with primary antibody ab5096 at a 1/100, in TBST with 1% serum for 1 hour at room temperature. After washing in TBST, slides were incubated with secondary antibody at 1/1000, for 30 minutes at room temperature. Cover slips were mounted with Prolong Gold Antifade with DAPI.

    Ovarian cord structure was examined by TRA98 germ cell and FOXL2 granulosa somatic cell immunofluorescence over 12 days of e12.5 female genital ridge transplantation. Oocytes and granulosa cells clustered together in defi
  • Anti-FOXL2 antibody (ab5096) at 0.5 µg/ml + Human heart lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 43 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Yamamoto A  et al. The mechanisms underlying the effects of AMH on Müllerian duct regression in male mice. J Vet Med Sci 80:557-567 (2018). Read more (PubMed: 29526868) »
  • Nakamura T  et al. Ovarian mast cells migrate toward ovary-fimbria connection in neonatal MRL/MpJ mice. PLoS One 13:e0196364 (2018). Read more (PubMed: 29684078) »
See all 25 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (ovary)
Permeabilization
No
Specification
ovary
Blocking step
10% donkey serum, 0.2% Triton x100, PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Prof. Shyamal Roy

Verified customer

Submitted Mar 31 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (Gonad)
Gel Running Conditions
Reduced Denaturing (10% SDA-PAGE)
Loading amount
75 µg
Specification
Gonad
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 23 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Ovary)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Ovary
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 14 2015

Answer

Merci pour votre réponse.

J'ai comme convenu mis en place l'envoi d'une unité de ab5096. Le numéro de commande de remplacement gratuit de ce produit est *****.
Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Question
Answer

A ma connaissance, le test en ICC/IF des anticorps cités dans votre email n'est malheureusement pas prévu.

Personnellement j'essaierais à nouveau le ab5096. Si le problème persiste je conseillerai dans ce cas de tester le ab74144, spécifique de la forme phosphorylée (S263), testé en Western Blot et ELISA.

Read More

Answer

Merci pour votre réponse.

Malheureusement il ne nous est pas possible de fournir des échantillons de chaque référence car nous avons plus de 80.000 références anticorps et pour des raisons logistiques nous ne pouvons stocker différents volumes pour nos produits.

Le lot GR49557 qui a fonctionnait provient du même animal que le dernier lot testé, ce n'est que l'aliquotage qui diffère.

Plusieurs options sont possibles:
1) vous envoyer un nouveau tube de ab5096 d'un autre aliquotage (autre lot mais du même batch de production que GR49557 et GR86870).
2) vous envoyer un tube de ab74144, spécifique de la forme phosphorylée (S263), testé en Western Blot et ELISA, https://www.abcam.com/ab74144. Cet anticorps est en stock.
3) vous envoyer un tube de ab58622, monoclonal de souris testé en Western Blot et Dot Blot, https://www.abcam.com/ab58622. Cet anticorps est en stock.
4) vous envoyer un tube de ab68339, testé en Western Blot et ELISA, https://www.abcam.com/ab68339. Délai de livraison d'environ 1 semaine.
5) vous envoyer un tube de ab49293, testé en Western Blot et ELISA, https://www.abcam.com/ab49293. Délai de livraison d'environ 2 semaines.

Merci de m'indiquer la résolution qui vous conviendrait le mieux. Bien entendu, si le problème persiste nous pourrons envisager une autre solution.

Read More

Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le dernier tube de ab5096 que vous avez reçu ne fonctionne pas comme attendu.

Afin de mieux comprendre le problème et vous apporter la meilleure résolution, pourriez-vous me communiquer le numéro du lot précédent que vous avez utilisé et qui a bien fonctionné?

Nous n'avons malheureusement pour l'instant en stock une série de tubes de ab5096 qui proviennent du même batch de production. Le problème est peut-être lié au transport et non au lot.

Nous avons d'autres anti-FOXL2 mais ceux-ci n'ont pas encore été testés ni en IHC-P ni chez la souris:
ab74144, https://www.abcam.com/ab74144
ab49293, https://www.abcam.com/ab49293
ab68339, https://www.abcam.com/ab68339
ab58622, https://www.abcam.com/ab58662

Si un remplacement n'est pas la meilleure option nous pouvons toujours vous envoyer un avoir ou un remboursement.

Merci de m'indiquer le numéro du lot qui a bien fonctionné et, si votre décision est prise, la résolution qui vous conviendrait le mieux.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (DU145 (prostate cancer cell line)
Loading amount
10 µg
Specification
DU145 (prostate cancer cell line
Gel Running Conditions
Reduced Denaturing (7% tris-acetate)
Blocking step
Milk as blocking agent for 35 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jun 24 2008

Answer

Thank you for your enquiry. I am sorry but we do not currently have the synthetic immunogen peptide used to raise FOXL2 antibody (ab5096). The immunogen was a peptide of sequence DSKTGALHSRLDL. I am not aware of any modifications that were used in the synthesis of this product. I am sorry that I cannot be of more assistance.

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