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    foxl2-antibody-ab5096.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box
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Anti-FOXL2 antibody (ab5096)

  • Datasheet
  • SDS
Reviews (6)Q&A (5)References (40)

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Western blot - Anti-FOXL2 antibody (ab5096)

    Key features and details

    • Goat polyclonal to FOXL2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-FOXL2 antibody [EPR23523-68] (ab246511)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-FOXL2 antibody
      See all FOXL2 primary antibodies
    • Description

      Goat polyclonal to FOXL2
    • Host species

      Goat
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to FOXL2 aa 364-376 (C terminal).
      Sequence:

      DSKTGALHSRLDL

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: K562 and HeLa cell lysates.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Forkhead Box
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Forkhead Box
      • Other FOXes

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab5096 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Cow
    Application Abreviews Notes
    WB (2)
    Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45-50 kDa (predicted molecular weight: 43 kDa).

    Primary incubation 1 hour at room temperature.

    Notes
    WB
    Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45-50 kDa (predicted molecular weight: 43 kDa).

    Primary incubation 1 hour at room temperature.

    Target

    • Function

      Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
    • Tissue specificity

      In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
    • Involvement in disease

      Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
      Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
    • Sequence similarities

      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications

      Sumoylated by SUMO1; sumoylation is required for transcriptional repression activity.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P58012 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 281770 Cow
      • Entrez Gene: 668 Human
      • Entrez Gene: 26927 Mouse
      • Entrez Gene: 367152 Rat
      • Omim: 605597 Human
      • SwissProt: Q6VFT7 Cow
      • SwissProt: P58012 Human
      • SwissProt: O88470 Mouse
      • Unigene: 289292 Human
      • Unigene: 151239 Mouse
      see all
    • Alternative names

      • Blepharophimosis antibody
      • Blepharophimosis epicanthus inversus and ptosis 1 antibody
      • Blepharophimosis epicanthus inversus and ptosis antibody
      • BPES 1 antibody
      • BPES antibody
      • BPES1 antibody
      • Epicanthus inversus and ptosis 1 antibody
      • Forkhead box L2 antibody
      • Forkhead box protein L2 antibody
      • Forkhead transcription factor FOXL2 antibody
      • FOX L2 antibody
      • FOXL 2 antibody
      • FOXL2 antibody
      • FOXL2_HUMAN antibody
      • PFRK antibody
      • PINTO antibody
      • PITUITARY FORKHEAD FACTOR antibody
      • POF 3 antibody
      • POF3 antibody
      see all

    Images

    • Western blot - Anti-FOXL2 antibody (ab5096)
      Western blot - Anti-FOXL2 antibody (ab5096)
      All lanes : Anti-FOXL2 antibody (ab5096) at 2 µg/ml

      Lane 1 : K562 cell lysate, 35µg protein in RIPA buffer
      Lanes 2 & 4 : K562 cell lysate incubated with immunizing peptide, 35µg protein in RIPA buffer
      Lane 3 : HeLa cell lysate, 35µg protein in RIPA buffer

      Developed using the ECL technique.

      Predicted band size: 43 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?



      Primary incubation 1 hour at room temperature.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (40)

    Publishing research using ab5096? Please let us know so that we can cite the reference in this datasheet.

    ab5096 has been referenced in 40 publications.

    • Guioli S  et al. Oestrogen in the chick embryo can induce chromosomally male ZZ left gonad epithelial cells to form an ovarian cortex that can support oogenesis. Development 147:N/A (2020). PubMed: 32001442
    • Hasegawa C  et al. Establishment of an organ culture system to induce Sertoli cell differentiation from undifferentiated mouse gonads. J Vet Med Sci 82:414-421 (2020). PubMed: 32092744
    • Wen J  et al. GSK-3ß protects fetal oocytes from premature death via modulating TAp63 expression in mice. BMC Biol 17:23 (2019). PubMed: 30866939
    • Yokoyama T  et al. Genetic differences between C57BL/6 substrains affect the process of testis differentiation in YPOS mice. J Vet Med Sci 81:608-611 (2019). PubMed: 30828038
    • Wu J  et al. Estrogen regulates forkhead transcription factor 2 to promote apoptosis of human ovarian granulosa-like tumor cells. J Steroid Biochem Mol Biol 194:105418 (2019). PubMed: 31376461
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 11 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXL2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (uterus tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH6 citrate/citric acid
    Permeabilization
    No
    Specification
    uterus tumor
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 01 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-FOXL2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin/Hair Follicle)
    Permeabilization
    No
    Specification
    Skin/Hair Follicle
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted May 10 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FOXL2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Hamster Cell (ovary)
    Permeabilization
    No
    Specification
    ovary
    Blocking step
    10% donkey serum, 0.2% Triton x100, PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Prof. Shyamal Roy

    Verified customer

    Submitted Mar 31 2016

    Western blot abreview for Anti-FOXL2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Gonad)
    Gel Running Conditions
    Reduced Denaturing (10% SDA-PAGE)
    Loading amount
    75 µg
    Specification
    Gonad
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 23 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXL2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Ovary)
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Specification
    Ovary
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 14 2015

    Question

    Bonjour,
    Effectivement, après concertation, nous aimerions retester le ab5096. Serait-il possible de nous envoyer un nouveau produit?
    Avec tous nos remerciements.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2012

    Answer

    Merci pour votre réponse.

    J'ai comme convenu mis en place l'envoi d'une unité de ab5096. Le numéro de commande de remplacement gratuit de ce produit est *****.
    Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

    N'hésitez pas à nous contacter lors d'une prochaine occasion.

    Read More

    Abcam Scientific Support

    Answered on Aug 08 2012

    Question

    Allez-vous tester les références ab58622, ab68339 et ab49293 en IF?
    Cordialement

    Read More

    Abcam community

    Verified customer

    Asked on Aug 03 2012

    Answer

    A ma connaissance, le test en ICC/IF des anticorps cités dans votre email n'est malheureusement pas prévu.

    Personnellement j'essaierais à nouveau le ab5096. Si le problème persiste je conseillerai dans ce cas de tester le ab74144, spécifique de la forme phosphorylée (S263), testé en Western Blot et ELISA.

    Read More

    Abcam Scientific Support

    Answered on Aug 03 2012

    Question

    Bonjour,
    Merci pour votre réponse.
    Nous avons commandé plusieurs fois cet anticorps et le lot du dernier en date (qui fonctionnait) est le GR49557.
    Serait-il possible d'avoir un échantillon des nouveaux anticorps non tester afin de les tester sur coupe paraffine et d'en choisir un à commander?
    Avec tous mes remerciements.
    Cordialement.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 03 2012

    Answer

    Merci pour votre réponse.

    Malheureusement il ne nous est pas possible de fournir des échantillons de chaque référence car nous avons plus de 80.000 références anticorps et pour des raisons logistiques nous ne pouvons stocker différents volumes pour nos produits.

    Le lot GR49557 qui a fonctionnait provient du même animal que le dernier lot testé, ce n'est que l'aliquotage qui diffère.

    Plusieurs options sont possibles:
    1) vous envoyer un nouveau tube de ab5096 d'un autre aliquotage (autre lot mais du même batch de production que GR49557 et GR86870).
    2) vous envoyer un tube de ab74144, spécifique de la forme phosphorylée (S263), testé en Western Blot et ELISA, https://www.abcam.com/ab74144. Cet anticorps est en stock.
    3) vous envoyer un tube de ab58622, monoclonal de souris testé en Western Blot et Dot Blot, https://www.abcam.com/ab58622. Cet anticorps est en stock.
    4) vous envoyer un tube de ab68339, testé en Western Blot et ELISA, https://www.abcam.com/ab68339. Délai de livraison d'environ 1 semaine.
    5) vous envoyer un tube de ab49293, testé en Western Blot et ELISA, https://www.abcam.com/ab49293. Délai de livraison d'environ 2 semaines.

    Merci de m'indiquer la résolution qui vous conviendrait le mieux. Bien entendu, si le problème persiste nous pourrons envisager une autre solution.

    Read More

    Abcam Scientific Support

    Answered on Aug 03 2012

    Question

    Product code: 5096
    Lot number: GR86870-1

    Inquiry: Bonjour, Lundi 16 juillet, nous avons reçu l'anticorps FoxL2. Mais il n’a pas fonctionné comme nous avons déjà pu le faire précédemment. Vous trouverez en fichier joint ce que j'ai obtenu (photo "FoxL2" qui est donc surexposée). Un contrôle positif a été utilisé pour valider l’expérience. Echantillon: tissus de souris Protocole utilisé: 2x10min Xylène hydratation ethanol epitope umasking Serum Blocking 1 heure Serum/BSA/Tween/PBS Anticorps FoxL2 over night 4°C dilution 1/50° et 1/100° Lavage PBS/Tween Anticorps secondaire fluorescent 1/150° température ambiante Lavage PBS/Tween Dapi Serait-il possible de nous envoyer un Anticorps Foxl2 d'un autre lot? Avec tous mes remerciements.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 02 2012

    Answer

    Merci de nous avoir contactés.

    Nous sommes désolés d'apprendre que le dernier tube de ab5096 que vous avez reçu ne fonctionne pas comme attendu.

    Afin de mieux comprendre le problème et vous apporter la meilleure résolution, pourriez-vous me communiquer le numéro du lot précédent que vous avez utilisé et qui a bien fonctionné?

    Nous n'avons malheureusement pour l'instant en stock une série de tubes de ab5096 qui proviennent du même batch de production. Le problème est peut-être lié au transport et non au lot.

    Nous avons d'autres anti-FOXL2 mais ceux-ci n'ont pas encore été testés ni en IHC-P ni chez la souris:
    ab74144, https://www.abcam.com/ab74144
    ab49293, https://www.abcam.com/ab49293
    ab68339, https://www.abcam.com/ab68339
    ab58622, https://www.abcam.com/ab58662

    Si un remplacement n'est pas la meilleure option nous pouvons toujours vous envoyer un avoir ou un remboursement.

    Merci de m'indiquer le numéro du lot qui a bien fonctionné et, si votre décision est prise, la résolution qui vous conviendrait le mieux.

    Read More

    Abcam Scientific Support

    Answered on Aug 02 2012

    Western blot abreview for Anti-FOXL2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (DU145 (prostate cancer cell line)
    Loading amount
    10 µg
    Specification
    DU145 (prostate cancer cell line
    Gel Running Conditions
    Reduced Denaturing (7% tris-acetate)
    Blocking step
    Milk as blocking agent for 35 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 24 2008

    1-10 of 11 Abreviews or Q&A

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