Overview

  • Product name

    Anti-FOXL2 antibody [EPR4837]
    See all FOXL2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4837] to FOXL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FOXL2 aa 1-100. The exact sequence is proprietary.
    Database link: P58012

  • Positive control

    • Jurkat, K562 and Human testis lysates; A549 cell extract.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).

Target

  • Function

    Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
  • Tissue specificity

    In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
  • Involvement in disease

    Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
    Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications

    Sumoylated by SUMO1; sumoylation is required for transcriptional repression activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Blepharophimosis antibody
    • Blepharophimosis epicanthus inversus and ptosis 1 antibody
    • Blepharophimosis epicanthus inversus and ptosis antibody
    • BPES 1 antibody
    • BPES antibody
    • BPES1 antibody
    • Epicanthus inversus and ptosis 1 antibody
    • Forkhead box L2 antibody
    • Forkhead box protein L2 antibody
    • Forkhead transcription factor FOXL2 antibody
    • FOX L2 antibody
    • FOXL 2 antibody
    • FOXL2 antibody
    • FOXL2_HUMAN antibody
    • PFRK antibody
    • PINTO antibody
    • PITUITARY FORKHEAD FACTOR antibody
    • POF 3 antibody
    • POF3 antibody
    see all

Images

  • Anti-FOXL2 antibody [EPR4837] (ab188584) at 1/1000 dilution + Jurkat cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-FOXL2 antibody [EPR4837] (ab188584) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Human testis cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • All lanes : Anti-FOXL2 antibody [EPR4837] (ab188584) at 1/10000 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with immunizing peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Chai P  et al. Functional Analysis of a Novel FOXL2 Indel Mutation in Chinese Families with Blepharophimosis-Ptosis-Epicanthus Inversus Syndrome Type I. Int J Biol Sci 13:1019-1028 (2017). Read more (PubMed: 28924383) »
  • Li Q  et al. FOXL2 down-regulates vitellogenin expression at mature stage in Eriocheir sinensis. Biosci Rep 35:N/A (2015). Read more (PubMed: 26430246) »
See all 2 Publications for this product

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