Anti-FOXM1 antibody (ab180710)
Key features and details
- Rabbit polyclonal to FOXM1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-FOXM1 antibody
See all FOXM1 primary antibodies -
Description
Rabbit polyclonal to FOXM1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human FOXM1 aa 1-350 (N terminal).
Database link: Q08050 -
Positive control
- WB: SW620, SKOV3, A-549, A-431 and HeLa cell lysates; Mouse heart, spleen and thymus tissue lysates; Rat thymus tissue lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 84 kDa.
|
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 84 kDa. |
Target
-
Function
Transcriptional activatory factor. May play a role in the control of cell proliferation. -
Tissue specificity
Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Developmental stage
Embryonic expression pattern: liver, lung, intestine, kidney, urinary tract; adult expression pattern: intestine, colon, testis and thymus. -
Domain
Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4. -
Post-translational
modificationsPhosphorylated in M (mitotic) phase. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 2305 Human
- Entrez Gene: 14235 Mouse
- Entrez Gene: 58921 Rat
- Omim: 602341 Human
- SwissProt: Q08050 Human
- SwissProt: O08696 Mouse
- SwissProt: P97691 Rat
- Unigene: 239 Human
see all -
Alternative names
- FKHL16 antibody
- Forkhead box M1 antibody
- Forkhead box protein M1 antibody
see all
Images
-
All lanes : Anti-FOXM1 antibody (ab180710) at 1/1000 dilution
Lane 1 : SW620 cell lysate
Lane 2 : SKOV3 cell lysate
Lane 3 : A-549 cell lysate
Lane 4 : A-431 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : Mouse heart tissue lysate
Lane 7 : Mouse spleen tissue lysate
Lane 8 : Mouse thymus tissue lysate
Lane 9 : Rat thymus tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 84 kDa
Exposure time: 90 seconds
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (30)
ab180710 has been referenced in 30 publications.
- Guo E et al. WEE1 inhibition induces anti-tumor immunity by activating ERV and the dsRNA pathway. J Exp Med 219:N/A (2022). PubMed: 34825915
- Hou Y et al. FOXM1 Promotes Drug Resistance in Cervical Cancer Cells by Regulating ABCC5 Gene Transcription. Biomed Res Int 2022:3032590 (2022). PubMed: 35141332
- Liu W et al. Suppression of CCT3 inhibits melanoma cell proliferation by downregulating CDK1 expression. J Cancer 13:1958-1971 (2022). PubMed: 35399722
- Lan C et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBioMedicine 82:104139 (2022). WB . PubMed: 35810562
- Zeng R et al. FOXM1 activates JAK1/STAT3 pathway in human osteoarthritis cartilage cell inflammatory reaction. Exp Biol Med (Maywood) 246:644-653 (2021). PubMed: 33297736