Recombinant Anti-FOXM1 antibody [EPR17379] (ab207298)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-FOXM1 antibody [EPR17379]
See all FOXM1 primary antibodies -
Description
Rabbit monoclonal [EPR17379] to FOXM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human FOXM1 aa 1-250. The exact sequence is proprietary.
Database link: Q08050 -
Positive control
- IHC-P: Human colon and colon cancer tissues. ICC/IF: HeLa and HT-29 cells. WB: MEF cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17379 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab207298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 84 kDa. | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| ICC/IF | 1/250. |
Target
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Function
Transcriptional activatory factor. May play a role in the control of cell proliferation. -
Tissue specificity
Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Developmental stage
Embryonic expression pattern: liver, lung, intestine, kidney, urinary tract; adult expression pattern: intestine, colon, testis and thymus. -
Domain
Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4. -
Post-translational
modificationsPhosphorylated in M (mitotic) phase. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2305 Human
- Omim: 602341 Human
- SwissProt: Q08050 Human
- Unigene: 239 Human
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Alternative names
- FKHL16 antibody
- Forkhead box M1 antibody
- Forkhead box protein M1 antibody
see all
Images
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![Western blot - Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-330938-anti-foxm1-antibody-epr17379-western-blot.jpg)
Western blot - Anti-FOXM1 antibody [EPR17379] (ab207298)All lanes : Anti-FOXM1 antibody [EPR17379] (ab207298) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : MEF (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-253248-anti-foxm1-antibody-epr17379-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on epithelial cells of Human colon is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-253244-anti-foxm1-antibody-epr17379-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on the Human colon cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-253243-anti-foxm1-antibody-epr17379-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR17379] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab207298 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-253242-anti-foxm1-antibody-epr17379-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HT-29 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR17379] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab207298 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Datasheets and documents
References
This product has been referenced in:
- Luo YD et al. FOXM1 is a novel predictor of recurrence in patients with oral squamous cell carcinoma associated with an increase in epithelial-mesenchymal transition. Mol Med Rep 19:4101-4108 (2019). Read more (PubMed: 30942437) »
- Haque M et al. NPM-ALK Is a Key Regulator of the Oncoprotein FOXM1 in ALK-Positive Anaplastic Large Cell Lymphoma. Cancers (Basel) 11:N/A (2019). Read more (PubMed: 31390744) »
