Recombinant Anti-FOXM1 antibody [EPR17379] (ab207298)
![Western blot - Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-330938-anti-foxm1-antibody-epr17379-western-blot.jpg "Western blot - Anti-FOXM1 antibody [EPR17379] (ab207298)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17379] to FOXM1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-FOXM1 antibody [EPR17379]
See all FOXM1 primary antibodies -
Description
Rabbit monoclonal [EPR17379] to FOXM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human FOXM1 aa 1-250. The exact sequence is proprietary.
Database link: Q08050 -
Positive control
- IHC-P: Human colon and colon cancer tissues. ICC/IF: HeLa and HT-29 cells. WB: MEF cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17379 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab207298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 84 kDa. | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| ICC/IF | 1/250. |
Target
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Function
Transcriptional activatory factor. May play a role in the control of cell proliferation. -
Tissue specificity
Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Developmental stage
Embryonic expression pattern: liver, lung, intestine, kidney, urinary tract; adult expression pattern: intestine, colon, testis and thymus. -
Domain
Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4. -
Post-translational
modificationsPhosphorylated in M (mitotic) phase. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2305 Human
- Entrez Gene: 14235 Mouse
- Omim: 602341 Human
- SwissProt: Q08050 Human
- SwissProt: O08696 Mouse
- Unigene: 239 Human
- Unigene: 42148 Mouse
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Alternative names
- FKHL16 antibody
- Forkhead box M1 antibody
- Forkhead box protein M1 antibody
see all
Images
-
Western blot - Anti-FOXM1 antibody [EPR17379] (ab207298)All lanes : Anti-FOXM1 antibody [EPR17379] (ab207298) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : MEF (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-253248-anti-foxm1-antibody-epr17379-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on epithelial cells of Human colon is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-253244-anti-foxm1-antibody-epr17379-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on the Human colon cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-253243-anti-foxm1-antibody-epr17379-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR17379] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab207298 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-253242-anti-foxm1-antibody-epr17379-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HT-29 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR17379] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab207298 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
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![Anti-FOXM1 antibody [EPR17379] (ab207298)](https://www.abcam.com/ps/products/207/ab207298/Images/ab207298-7-benefits-of-recombinant-antibodies.png)
Anti-FOXM1 antibody [EPR17379] (ab207298)
Protocols
Datasheets and documents
Certificate of Compliance
References (10)
ab207298 has been referenced in 10 publications.
- Bu H et al. Overexpression of PRC1 indicates a poor prognosis in ovarian cancer. Int J Oncol 56:685-696 (2020). PubMed: 31922238
- Li R et al. Centromere protein F and Forkhead box M1 correlation with prognosis of non-small cell lung cancer. Oncol Lett 19:1368-1374 (2020). PubMed: 31966068
- Cheng L et al. FOXM 1 induces Vasculogenic mimicry in esophageal cancer through ß-catenin /Tcf4 signaling. Diagn Pathol 15:14 (2020). PubMed: 32035486
- Nie S et al. Expression, association with clinicopathological features and prognostic potential of CEP55, p-Akt, FoxM1 and MMP-2 in astrocytoma. Oncol Lett 20:1685-1694 (2020). PubMed: 32724411
- Luo YD et al. FOXM1 is a novel predictor of recurrence in patients with oral squamous cell carcinoma associated with an increase in epithelial-mesenchymal transition. Mol Med Rep 19:4101-4108 (2019). PubMed: 30942437
- Haque M et al. NPM-ALK Is a Key Regulator of the Oncoprotein FOXM1 in ALK-Positive Anaplastic Large Cell Lymphoma. Cancers (Basel) 11:N/A (2019). PubMed: 31390744
- Liang J et al. Expression of FSCN1 and FOXM1 are associated with poor prognosis of adrenocortical carcinoma patients. BMC Cancer 19:1165 (2019). PubMed: 31783819
- Khan I et al. FOXM1 contributes to treatment failure in acute myeloid leukemia. JCI Insight 3:N/A (2018). PubMed: 30089730
- Feng Y et al. FOXM1 as a prognostic biomarker promotes endometrial cancer progression via transactivation of SLC27A2 expression. Int J Clin Exp Pathol 11:3846-3857 (2018). PubMed: 31949772
- Ma J et al. Overexpression of forkhead box M1 and urokinase-type plasminogen activator in gastric cancer is associated with cancer progression and poor prognosis. Oncol Lett 14:7288-7296 (2017). PubMed: 29344165
