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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box
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RecombinantRabMAb

Recombinant Anti-FOXM1 antibody [EPR17379] (ab207298)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (10)

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Western blot - Anti-FOXM1 antibody [EPR17379] (ab207298)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)
  • Anti-FOXM1 antibody [EPR17379] (ab207298)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17379] to FOXM1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

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Recombinant Human FOXM1 protein (ab158447)
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Overview

  • Product name

    Anti-FOXM1 antibody [EPR17379]
    See all FOXM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR17379] to FOXM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human FOXM1 aa 1-250. The exact sequence is proprietary.
    Database link: Q08050

  • Positive control

    • IHC-P: Human colon and colon cancer tissues. ICC/IF: HeLa and HT-29 cells. WB: MEF cell lysate.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17379
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Other FOXes
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Alternative Versions

    • Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human FOXM1 protein (ab158447)

Applications

Our Abpromise guarantee covers the use of ab207298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 84 kDa.
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.

Target

  • Function

    Transcriptional activatory factor. May play a role in the control of cell proliferation.
  • Tissue specificity

    Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Developmental stage

    Embryonic expression pattern: liver, lung, intestine, kidney, urinary tract; adult expression pattern: intestine, colon, testis and thymus.
  • Domain

    Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4.
  • Post-translational
    modifications

    Phosphorylated in M (mitotic) phase.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q08050 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2305 Human
    • Entrez Gene: 14235 Mouse
    • Omim: 602341 Human
    • SwissProt: Q08050 Human
    • SwissProt: O08696 Mouse
    • Unigene: 239 Human
    • Unigene: 42148 Mouse
    • Alternative names

      • FKHL16 antibody
      • Forkhead box M1 antibody
      • Forkhead box protein M1 antibody
      • forkhead like 16 antibody
      • Forkhead-related protein FKHL16 antibody
      • FOX M1 antibody
      • Foxm1 antibody
      • FOXM1_HUMAN antibody
      • FOXM1B antibody
      • Hepatocyte nuclear factor 3 forkhead homolog 11 antibody
      • HFH-11 antibody
      • HFH11 antibody
      • HNF-3/fork-head homolog 11 antibody
      • HNF3 antibody
      • INS1 antibody
      • M phase phosphoprotein 2 antibody
      • M-phase phosphoprotein 2 antibody
      • MPHOSPH2 antibody
      • MPM-2 reactive phosphoprotein 2 antibody
      • MPP2 antibody
      • PIG29 antibody
      • TGT3 antibody
      • Transcription factor Trident antibody
      • Trident antibody
      • WIN antibody
      • Winged-helix factor from INS-1 cells antibody
      • Winged-helix factor from INS1 cells antibody
      see all

    Images

    • Western blot - Anti-FOXM1 antibody [EPR17379] (ab207298)
      Western blot - Anti-FOXM1 antibody [EPR17379] (ab207298)
      All lanes : Anti-FOXM1 antibody [EPR17379] (ab207298) at 1/1000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : MEF (Mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 84 kDa
      Observed band size: 90 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on epithelial cells of Human colon is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] (ab207298)

      Immunohistochemical analysis of paraffin-embedded Human colon cancer  tissue labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on the Human colon cancer is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)
      Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

       

      Confocal image showing nuclear staining on HeLa cell line. 

       

      The nuclear counterstain is DAPI (blue).

       

      Tubulin is detected with Anti-alpha Tubulin antibody [EPR17379] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

       

      The negative controls are as follows:-

      -ve control 1: ab207298 at 1/250 dilution followed by ab150120  at 1/1000 dilution.

      -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)
      Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] (ab207298)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

       

      Confocal image showing nuclear staining on HT-29 cell line.

       

      The nuclear counterstain is DAPI (blue).

       

      Tubulin is detected with Anti-alpha Tubulin antibody [EPR17379] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

       

      The negative controls are as follows:-

      -ve control 1: ab207298 at 1/250 dilution followed by ab150120 at 1/1000 dilution.

      -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    • Anti-FOXM1 antibody [EPR17379] (ab207298)
      Anti-FOXM1 antibody [EPR17379] (ab207298)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (10)

    Publishing research using ab207298? Please let us know so that we can cite the reference in this datasheet.

    ab207298 has been referenced in 10 publications.

    • Bu H  et al. Overexpression of PRC1 indicates a poor prognosis in ovarian cancer. Int J Oncol 56:685-696 (2020). PubMed: 31922238
    • Li R  et al. Centromere protein F and Forkhead box M1 correlation with prognosis of non-small cell lung cancer. Oncol Lett 19:1368-1374 (2020). PubMed: 31966068
    • Cheng L  et al. FOXM 1 induces Vasculogenic mimicry in esophageal cancer through ß-catenin /Tcf4 signaling. Diagn Pathol 15:14 (2020). PubMed: 32035486
    • Nie S  et al. Expression, association with clinicopathological features and prognostic potential of CEP55, p-Akt, FoxM1 and MMP-2 in astrocytoma. Oncol Lett 20:1685-1694 (2020). PubMed: 32724411
    • Luo YD  et al. FOXM1 is a novel predictor of recurrence in patients with oral squamous cell carcinoma associated with an increase in epithelial-mesenchymal transition. Mol Med Rep 19:4101-4108 (2019). PubMed: 30942437
    • Haque M  et al. NPM-ALK Is a Key Regulator of the Oncoprotein FOXM1 in ALK-Positive Anaplastic Large Cell Lymphoma. Cancers (Basel) 11:N/A (2019). PubMed: 31390744
    • Liang J  et al. Expression of FSCN1 and FOXM1 are associated with poor prognosis of adrenocortical carcinoma patients. BMC Cancer 19:1165 (2019). PubMed: 31783819
    • Khan I  et al. FOXM1 contributes to treatment failure in acute myeloid leukemia. JCI Insight 3:N/A (2018). PubMed: 30089730
    • Feng Y  et al. FOXM1 as a prognostic biomarker promotes endometrial cancer progression via transactivation of SLC27A2 expression. Int J Clin Exp Pathol 11:3846-3857 (2018). PubMed: 31949772
    • Ma J  et al. Overexpression of forkhead box M1 and urokinase-type plasminogen activator in gastric cancer is associated with cancer progression and poor prognosis. Oncol Lett 14:7288-7296 (2017). PubMed: 29344165

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunoprecipitation abreview for Anti-FOXM1 antibody [EPR17379]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Osteosarcoma)
    Total protein in input
    500 µg
    Treatment
    Exogenous expression of GFP-FOXM1 fusion protein. Protein crosslinking with 25 µM DSP for 45 minutes.
    Immuno-precipitation step
    Protein A
    Specification
    Osteosarcoma
    Read More

    Dr. Mikhail Chesnokov

    Verified customer

    Submitted Mar 31 2020

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