Key features and details
- Goat polyclonal to FOXN1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab26069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 20 - 30 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionTranscriptional regulator involved in development.
Tissue specificityExpressed in thymus.
Involvement in diseaseDefects in FOXN1 are the cause of T-cell immunodeficiency congenital alopecia and nail dystrophy (TIDAND) [MIM:601705]. A disorder characterized by the association of congenital alopecia, severe T-cell immunodeficiency, and ridging and pitting of all nails.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
- Information by UniProt
- FKHL20 antibody
- Forkhead box N1 antibody
- Forkhead box protein N1 antibody
Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human skin tissue sections, labelling FOXN1 with ab26069 at a dilution of 1/25 incubated for 18 hours at 4°C. Permeablization was with 0.05% Tween. Blocking was with 5% serum incubated for 20 minutes at room temperature. The secondary used was a swine polyclonal biotin-conjugate at 1/1000.
Ab26069 (20ug/ml) staining human FOXN1 in human thymus medulla tissue by immunohistochemistry using paraffin embedded tissue.
Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
In paraffin embedded Human Thymus Medulla shows speculate nuclear staining in some of the Medullar cells.
ab26069 has been referenced in 2 publications.
- Suzuki S et al. Transcriptional and histological analyses of the thymic developmental process in the fetal pig. Exp Anim 63:215-25 (2014). IHC ; Pig . PubMed: 24770647
- Weissferdt A & Moran CA Thymic carcinoma, part 1: a clinicopathologic and immunohistochemical study of 65 cases. Am J Clin Pathol 138:103-14 (2012). IHC-P ; Human . PubMed: 22706865