Product nameAnti-FOXN3 antibody
See all FOXN3 primary antibodies
DescriptionRabbit polyclonal to FOXN3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to Human FOXN3 aa 440-490.
Database link: NP_001078940.1
- WB: Jurkat, HEK-293T and HeLa whole cell lysates.
This product was previously labelled as CHES1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab129453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 54 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints).
Sequence similaritiesContains 1 fork-head DNA-binding domain.
- Information by UniProt
- C14orf116 antibody
- Checkpoint suppressor 1 antibody
- CHES1 antibody
All lanes : Anti-FOXN3 antibody (ab129453) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3 minutes
Detection of FOXN3 by Western Blot of Immunprecipitate.
Lane 1: ab129453 at 1µg/ml staining FOXN3 in HeLa whole cell lysate immunoprecipitated using ab129453 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
This product has been referenced in:
- Xu Z et al. Checkpoint suppressor 1 suppresses transcriptional activity of ERa and breast cancer cell proliferation via deacetylase SIRT1. Cell Death Dis 9:559 (2018). Read more (PubMed: 29752474) »
- Sun M et al. MicroRNA-378 regulates epithelial-mesenchymal transition and metastasis of melanoma by inhibiting FOXN3 expression through the Wnt/ß-catenin pathway. Cell Biol Int N/A:N/A (2018). Read more (PubMed: 29972255) »