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  1. Link

    foxn3-antibody-ab129453.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box Other FOXes
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Anti-FOXN3 antibody (ab129453)

  • Datasheet
Submit a review Submit a question References (4)

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Western blot - Anti-FOXN3 antibody (ab129453)
  • Immunoprecipitation - Anti-FOXN3 antibody (ab129453)

Key features and details

  • Rabbit polyclonal to FOXN3
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human FOXN3 protein (ab158108)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-FOXN3 antibody
    See all FOXN3 primary antibodies
  • Description

    Rabbit polyclonal to FOXN3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human FOXN3 aa 440-490.
    Database link: NP_001078940.1

  • Positive control

    • WB: Jurkat, HEK-293T and HeLa whole cell lysates.
  • General notes

     This product was previously labelled as CHES1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS

    pH: 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Other FOXes
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human FOXN3 protein (ab158108)

Applications

Our Abpromise guarantee covers the use of ab129453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints).
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O00409 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1112 Human
    • Entrez Gene: 733584 Pig
    • Omim: 602628 Human
    • SwissProt: O00409 Human
    • SwissProt: Q33BP8 Pig
    • Unigene: 434286 Human
    • Unigene: 621371 Human
    • Alternative names

      • C14orf116 antibody
      • Checkpoint suppressor 1 antibody
      • CHES1 antibody
      • Forkhead box protein N3 antibody
      • Foxn3 antibody
      • FOXN3_HUMAN antibody
      • FXN3 antibody
      • PRO1635 antibody
      see all

    Images

    • Western blot - Anti-FOXN3 antibody (ab129453)
      Western blot - Anti-FOXN3 antibody (ab129453)
      All lanes : Anti-FOXN3 antibody (ab129453) at 0.1 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : HeLa whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 54 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-FOXN3 antibody (ab129453)
      Immunoprecipitation - Anti-FOXN3 antibody (ab129453)

      Detection of FOXN3 by Western Blot of Immunprecipitate.
      Lane 1: ab129453 at 1µg/ml staining FOXN3 in HeLa whole cell lysate immunoprecipitated using ab129453 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Lane 2: Control IgG
      Detection: Chemiluminescence with exposure time of 30 seconds.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab129453? Please let us know so that we can cite the reference in this datasheet.

    ab129453 has been referenced in 4 publications.

    • He H  et al. Novel tumor-suppressor FOXN3 is downregulated in adult acute myeloid leukemia. Oncol Lett 18:1521-1529 (2019). PubMed: 31423219
    • Xu Z  et al. Checkpoint suppressor 1 suppresses transcriptional activity of ERa and breast cancer cell proliferation via deacetylase SIRT1. Cell Death Dis 9:559 (2018). PubMed: 29752474
    • Sun M  et al. MicroRNA-378 regulates epithelial-mesenchymal transition and metastasis of melanoma by inhibiting FOXN3 expression through the Wnt/ß-catenin pathway. Cell Biol Int N/A:N/A (2018). PubMed: 29972255
    • Li W  et al. The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer. J Clin Invest 127:3421-3440 (2017). PubMed: 28805661

    Customer reviews and Q&As

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