Anti-FOXN3 antibody (ab129453)

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Overview

  • Product name

    Anti-FOXN3 antibody
    See all FOXN3 primary antibodies

  • Description

    Rabbit polyclonal to FOXN3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus

  • Immunogen

    Synthetic peptide corresponding to Human FOXN3 aa 440-490.
    Database link: NP_001078940.1

  • Positive control

    • WB: Jurkat, HEK-293T and HeLa whole cell lysates.

  • General notes

     This product was previously labelled as CHES1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab129453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • Western blot - Anti-FOXN3 antibody (ab129453)
    Western blot - Anti-FOXN3 antibody (ab129453)
    All lanes : Anti-FOXN3 antibody (ab129453) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 54 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-FOXN3 antibody (ab129453)
    Immunoprecipitation - Anti-FOXN3 antibody (ab129453)

    Detection of FOXN3 by Western Blot of Immunprecipitate.
    Lane 1: ab129453 at 1µg/ml staining FOXN3 in HeLa whole cell lysate immunoprecipitated using ab129453 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:

  • Xu Z  et al. Checkpoint suppressor 1 suppresses transcriptional activity of ERa and breast cancer cell proliferation via deacetylase SIRT1. Cell Death Dis 9:559 (2018). Read more (PubMed: 29752474) »
  • Sun M  et al. MicroRNA-378 regulates epithelial-mesenchymal transition and metastasis of melanoma by inhibiting FOXN3 expression through the Wnt/ß-catenin pathway. Cell Biol Int N/A:N/A (2018). Read more (PubMed: 29972255) »
See all 3 Publications for this product

Publishing research using ab129453? Please let us know so that we can cite the reference in this datasheet.

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