Product nameAnti-FOXO1A antibody
See all FOXO1A primary antibodies
DescriptionRabbit polyclonal to FOXO1A
Tested applicationsSuitable for: ICC/IF, ICC, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken, Pig
- ICC: mouse E11 cortical cells cultured for 6 days WB: HEK293 cell lysate, HeLa whole cell lysate (ab150035)
General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab12161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|ICC||1/200 - 1/1000.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/2500. Detects a band of approximately 70-75 kDa (predicted molecular weight: 70 kDa).|
FunctionTranscription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
Involvement in diseaseDefects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
modificationsPhosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
- Information by UniProt
- FKH 1 antibody
- FKH1 antibody
- FKHR antibody
All lanes : Anti-FOXO1A antibody (ab12161) at 1 µg/ml
Lane 1 :
HEK293 whole cell lysate (ab7902)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa (possible cross reactivity)
ab12161 staining FOXO1A (1/200) in mouse E11 cortical cells cultured for 6 days. Secondary antibody Alexa 546 goat anti rabbit IgG 1/1000 was incubated for 2 hours at room temperature. Images were taken using a Zeiss camera under 40X objectives.
ab12161 staining FOXOA1 (1/200) in the cortical plate (CP) and ventricular zone (VZ=CNS germinal zone) of mouse E13 forebrain tissue. E13 mouse embryos were fixed in 4% paraformaldehyde in PB overnight at 4°C then transferred to 30% sucrose in PBS. Brains were frozen in TissueTek and stored at -80°C. Cryostat coronal sections were cut at 12
µm. Sections were blocked in 0.1% triton in PBS containing 10% normal serum. Primary antibodies were incubated on the sections at 4°C overnight. Secondary antibody Alexa 546 goat anti rabbit IgG 1/1000 was incubated for 2 hours at room temperature. Images were taken using a Zeiss camera under 40X objectives.
This product has been referenced in:
- Savikj M et al. Retained differentiation capacity of human skeletal muscle satellite cells from spinal cord-injured individuals. Physiol Rep 6:e13739 (2018). Read more (PubMed: 29906337) »
- Picard M et al. Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional reprogramming. Proc Natl Acad Sci U S A 111:E4033-42 (2014). WB . Read more (PubMed: 25192935) »