• Product name
  • Description
    Rabbit polyclonal to FOXO1A
  • Host species
  • Tested applications
    Suitable for: ICC/IF, ICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Pig
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 636-651 of Human FOXO1A.

  • Positive control
    • ICC: mouse E11 cortical cells cultured for 6 days WB: HEK293 cell lysate, HeLa whole cell lysate (ab150035)
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.



Our Abpromise guarantee covers the use of ab12161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ICC 1/200 - 1/1000.
IHC-Fr Use at an assay dependent concentration.
WB 1/2500. Detects a band of approximately 70-75 kDa (predicted molecular weight: 70 kDa).


  • Function
    Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • Tissue specificity
  • Involvement in disease
    Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all


  • All lanes : Anti-FOXO1A antibody (ab12161) at 1 µg/ml

    Lane 1 : HEK293 whole cell lysate (ab7902)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 105 kDa (possible cross reactivity)

  • ab12161 staining FOXO1A (1/200) in mouse E11 cortical cells cultured for 6 days. Secondary antibody Alexa 546 goat anti rabbit IgG 1/1000 was incubated for 2 hours at room temperature. Images were taken using a Zeiss camera under 40X objectives.
  • ab12161 staining FOXOA1 (1/200) in the cortical plate (CP) and ventricular zone (VZ=CNS germinal zone) of mouse E13 forebrain tissue. E13 mouse embryos were fixed in 4% paraformaldehyde in PB overnight at 4°C then transferred to 30% sucrose in PBS.  Brains were frozen in TissueTek and stored at -80°C. Cryostat coronal sections were cut at 12µm. Sections were blocked in 0.1% triton in PBS containing 10% normal serum. Primary antibodies were incubated on the sections at 4°C overnight.  Secondary antibody Alexa 546 goat anti rabbit IgG 1/1000 was incubated for 2 hours at room temperature. Images were taken using a Zeiss camera under 40X objectives.


This product has been referenced in:
  • Savikj M  et al. Retained differentiation capacity of human skeletal muscle satellite cells from spinal cord-injured individuals. Physiol Rep 6:e13739 (2018). Read more (PubMed: 29906337) »
  • Picard M  et al. Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional reprogramming. Proc Natl Acad Sci U S A 111:E4033-42 (2014). WB . Read more (PubMed: 25192935) »
See all 9 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Thank you for your email. I have instructed our accounting department to issue you a refund for your order for ab12161. As the order was placed using a purchase order number, this process does take a little while. If you have any questions regarding the refund, please contact accounts@abcam.com Thank you, and have a nice weekend.

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Thank you for your email. I can send you a free of charge replacement vial of ab12161, or I can issue you a credit note or refund. Please let me know which you would prefer. Thank you, and I look forward to hearing from you.

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Thank you for your email and the image that you sent. There is a definite improvement with regards to background and non-specific banding. Did you filter the TBST buffer that you are using? Failure to filter can lead to “spotting” where tiny dark grains will contaminate the blot during development. If you have not filtered this, we strongly suggest that you try this. If you already have, or it does not improve your results, we will gladly offer to send you a replacement vial free of charge or will issue you a refund or credit note. Thank you again, and we look forward to hearing from you.

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Thank you for your email and for sending images of your blots. I can definitely see that you are experiencing difficulty with high background and non-specific bands. With this antibody you should see a band at approximately 70-75 kDa. It would be helpful if you could provide molecular weight markers on your images which would give me a clearer view if you are actually seeing a band at this size or not. Also, you mentioned that you were using cytosolic fractions of mice prostate tumor. I would suggest using nuclear extracts as the cellular localization of FOXO1A is nuclear. Using nuclear extracts should help clean up your blot. For me to investigate further, I really do need additional protocol details from you. What dilutions of ab12161 have you tried? How long were the incubation periods? What is your secondary antibody - what dilutions were used and how long did you incubate for? What are you blocking with and for how long? Thank you, and I look forward to hearing from you.

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Thank you for your enquiry. All the information that we have regarding these two antibodies is located on the online datasheets. We currently do not have information about specificity to O1A/o1a and other FOXO/foxo family members. If you have any more questions, please contact us again.

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